Comparative antiplasmodial activity, cytotoxicity, and phytochemical contents of Warburgia ugandensis stem bark against Aspilia africana wild and in vitro regenerated tissues
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Malaria remains to be one of the most severe global public health concerns. Traditionally, Aspilia africana and Warburgia ugandensis have been used to treat malaria in several African countries for millennia. In the current study, A. africana calli (AaC), A. africana in vitro roots (AaIR), A. africana wild leaf (AaWL), and W. ugandensis stem bark (WuSB) were dried and pulverized. Fourier transform near-infrared spectroscopy was used to analyze the powdered samples, while 80% ethanolic extracts of each sample were assayed for antiplasmodial activity (against Plasmodium falciparum strains DD2 (chloroquine-resistant) and 3D7 (chloroquine-sensitive)) and cytotoxicity. WuSB showed the highest antiplasmodial activity (IC50 = 1.57 ± 0.210 µg/ml and 8.92 ± 0.365 µg/ml against P. falciparum 3D7 and DD2, respectively) and selectivity indices (43.90 ± 7.914 and 7.543 ± 0.051 for P. falciparum 3D7 and DD2, respectively). The highest total polyphenolic contents (total phenolic and flavonoid contents of 367.9 ± 3.55 mg GAE/g and 203.9 ± 1.43 mg RUE/g, respectively) were recorded for WuSB and the lowest were recorded for AaC. The anti- plasmodial activities of the tested plant tissues correlated positively with total polyphenolic content. The high selectivity indices of WuSB justify its traditional applications in treating malaria and present it as a good candidate for discovering new antimalarial compounds. We recommend elicitation treatment for AaIR, which showed moderate antiplasmodial activity against P. falciparum DD2, to increase its secondary metabolite production for optimal antimalarial activity.
Abstract
Malaria remains to be one of the most severe global public health concerns. Traditionally, Aspilia africana and Warburgia ugandensis have been used to treat malaria in several African countries for millennia. In the current study, A. africana calli (AaC), A. africana in vitro roots (AaIR), A. africana wild leaf (AaWL), and W. ugandensis stem bark (WuSB) were dried and pulverized. Fourier transform near-infrared spectroscopy was used to analyze the powdered samples, while 80% ethanolic extracts of each sample were assayed for antiplasmodial activity (against Plasmodium falciparum strains DD2 (chloroquine-resistant) and 3D7 (chloroquine-sensitive)) and cytotoxicity. WuSB showed the highest antiplasmodial activity (IC50 = 1.57 ± 0.210 µg/ml and 8.92 ± 0.365 µg/ml against P. falciparum 3D7 and DD2, respectively) and selectivity indices (43.90 ± 7.914 and 7.543 ± 0.051 for P. falciparum 3D7 and DD2, respectively). The highest total polyphenolic contents (total phenolic and flavonoid contents of 367.9 ± 3.55 mg GAE/g and 203.9 ± 1.43 mg RUE/g, respectively) were recorded for WuSB and the lowest were recorded for AaC. The anti- plasmodial activities of the tested plant tissues correlated positively with total polyphenolic content. The high selectivity indices of WuSB justify its traditional applications in treating malaria and present it as a good candidate for discovering new antimalarial compounds. We recommend elicitation treatment for AaIR, which showed moderate antiplasmodial activity against P. falciparum DD2, to increase its secondary metabolite production for optimal antimalarial activity.
참고문헌 (59)
- Afagnigni AD, "LC-MS analysis, 15-lipoxygenase inhibition, cytotoxicity, and genotoxicity of dissotis multiflora(Sm)triana(melastomataceae)and Paullinia pinnata Linn(Sapindaceae)", J Trop Med, 2020 (2020)
- Ahuchaogu AA, "GC-MS analysis of bioactive compounds from whole plant chloroform extract of Ageratum conyzoides", IJMPNP, 4, pp.13~24 (2018)
- Andrade-Neto VF, "Antimalarial activity of Bidens pilosa L. (Asteraceae) ethanol extracts from wild plants collected in various localities or plants cultivated in humus soil", Phytother Res, 18(8), pp.634~639 (2004)
- Bantie L, "In vivo antimalarial activity of the crude leaf extract and solvent fractions of Croton macrostachyus Hocsht.(Euphorbiaceae) against Plasmodium berghei in mice", BMC Complement Altern Med, 14, pp.79~ (2014)
- Barber BE, "The treatment of Plasmodium knowlesi malaria", Trends Parasitol, 33(3), pp.242~253 (2017)
- Bero J, "In vitro antiplasmodial activity of plants used in Benin in traditional medicine to treat malaria", J Ethnopharmacol, 122(3), pp.439~444 (2009)
- Bucchini A, "Total polyphenol content, in vitro antifungal and antioxidant activities of callus cultures from Inula crithmoides", Nat Prod Commun, 8(11), pp.1934578~ (2013)[DOI]
- Carbas B, "Prediction of phytochemical composition, in vitro antioxidant activity and individual phenolic compounds of common beans using MIR and NIR spectroscopy", Food Bioproc Tech, 13, pp.962~977 (2020)
- Chono A, "Clinical and parasitological effects of Aspilia africana(Pers. )CD Adams in fifteen patients with uncomplicated malaria", East and Central African Journal of Pharmaceutical Sciences, 12(2) (2009)
- Christian AG, "Antimalarial potency of the leaf extract of Aspilia africana (Pers.) CD Adams", Asian Pac J Trop Med, 5, pp.126~129 (2012)
- Cimanga R, "In vitro antiplasmodial activity of callus culture extracts and fractions from fresh apical stems of Phyllanthus niruri L. (Euphorbiaceae) : part 2", J Ethnopharmacol, 95, pp.399~404 (2004)
- Cudjoe E, "The in vitro antiplasmodial activities of aqueous extracts of selected Ghanaian herbal plants", J Parasitol Res, 2020 (2020)
- Derakhshan Z, "Antioxidant activity and total phenolic content of ethanolic extract of pomegranate peels, juice and seeds", FTC, 114, pp.108~111 (2018)
- Dolabela MF, "In vitro antiplasmodial activity of extract and constituents from Esenbeckia febrifuga, a plant traditionally used to treat malaria in the Brazilian Amazon", Phytomedicine, 15, pp.367~372 (2008)
- Faraz R, "Callus extracts of Oroxylum indicum (L.) vent containing baicalein have in vitro antioxidant and antibacterial activities", Biotecnología Vegetal, 20, pp.51~62 (2020)
- Roggers Gang, "Botanical features and ethnopharmacological potential of Leonotis nepetifolia (L.) R. Br: a review", Journal of Plant Biotechnology, 49(1), pp.3~14 (2022), 한국식물생명공학회[DOI]
- Grąbkowska R, "Callus cultures of Harpagophytum procumbens (Burch.) DC. ex Meisn.; production of secondary metabolites and antioxidant activity", S Afr J Bot, 103, pp.41~48 (2016)
- Grace MH, "Phytochemical changes in phenolics, anthocyanins, ascorbic acid, and carotenoids associated with sweetpotato storage and impacts on bioactive properties", Food Chem, 145, pp.717~724 (2014)
- Ichino C, "Screening of Thai medicinal plant extracts and their active constituents for in vitro antimalarial activity", Phytother Res, 20, pp.307~309 (2006)
- Inbaneson SJ, "In vitro antiplasmodial effect of ethanolic extracts of traditional medicinal plant Ocimum species against Plasmodium falciparum", Asian Pac J Trop Med, 5, pp.103~106 (2012)
- Irungu BN, "In vitro antiplasmodial and cytotoxicity activities of 14 medicinal plants from Kenya", S Afr J Bot, 73, pp.204~207 (2007)
- Karama I, "Antiplasmodial activities of flavonoids from leaves of Securidaca longepedunculata Fresen(Polygalaceae)", AJBIO, 8, pp.1~5 (2020)
- Katuura E, "Traditional treatment of malaria in Mbarara District, western Uganda", Afr J Ecol, 45, pp.48~51 (2007)
- Makler M, "Parasite lactate dehydrogenase as an assay for Plasmodium falciparum drug sensitivity", Am J Trop Med Hyg, 48, pp.739~741 (1993)
- Mallik J, "Phytochemical screening and in-vitro evaluation of reducing power, cytotoxicity and anti-fungal activities of ethanol extracts of Cucumis sativus", Int J Pharm Biol Arch, 3, pp.555~560 (2012)
- Martinez-Correa HA, "Composition and antimalarial activity of extracts of Curcuma longa L. obtained by a combination of extraction processes using supercritical CO2, ethanol and water as solvents", J Supercrit Fluids, 119, pp.122~129 (2017)
- Mediani A, "Effects of different drying methods and storage time on free radical scavenging activity and total phenolic content of Cosmos caudatus", Antioxidants, 3, pp.358~370 (2014)
- Monroe A, "Reflections on the 2021 World Malaria Report and the future of malaria control", Malar J, 21(154), pp.1~6 (2022)
- Muller PY, "The determination and interpretation of the therapeutic index in drug development", Nat Rev Drug Discov, 11, pp.751~761 (2012)
- Mwitari PG, "Antimicrobial activity and probable mechanisms of action of medicinal plants of Kenya : Withania somnifera, Warbugia ugandensis, Prunus africana and Plectrunthus barbatus", PloS one, 8, pp.e65619~ (2013)
- Njeru SN, "In vitro cytotoxicity of Aspilia pluriseta Schweinf. extract fractions", BMC Research Notes, 14, pp.1~4 (2021)
- Njeru SN, "Antimicrobial and cytotoxicity properties of the crude extracts and fractions of Premna resinosa (Hochst.) Schauer (Compositae): Kenyan traditional medicinal plant", BMC Complement Altern Med, 15, pp.1~9 (2015)
- Ntie-Kang F, "The potential of anti-malarial compounds derived from African medicinal plants, part II : a pharmacological evaluation of non-alkaloids and non-terpenoids", Malar J, 13, pp.1~20 (2014)
- Okello D, "Exploring antimalarial herbal plants across communities in Uganda based on electronic data", eCAM, 2019 (2019)
- Okello D, "Ethnopharmacological potentials of Warburgia ugandensis on antimicrobial activities", Chin J Integr Med, 27, pp.633~640 (2021)
- Okello D, "Antioxidant activity, polyphenolic content, and FT-NIR analysis of different Aspilia africana medicinal plant tissues", eCAM, 2021 (2021)
- Okello D, "A review on the botanical aspects, phytochemical contents and pharmacological activities of Warburgia ugandensis", J Med Plant Res, 12, pp.448~455 (2018)
- Oliveira F, "New evidences of antimalarial activity of Bidens pilosa roots extract correlated with polyacetylene and flavonoids", J Ethnopharmacol, 93, pp.39~42 (2004)
- Pandey V, "Efficient micropropagation protocol of Spilanthes acmella L. possessing strong antimalarial activity", In Vitro Cell Dev Biol, 45, pp.491~499 (2009)
- Páscoa RN, "Antioxidant capacity of Camellia japonica cultivars assessed by near-and mid-infrared spectroscopy", Planta, 249, pp.1053~1062 (2019)
- Putri NS, "Callus culture as the method in providing antimalarial compounds of Piper genus", J Environ Sci Sustain Soc, 10(3), pp.03~11 (2021)
- Rajendran R, "Isolation and quantification of antimalarial N-alkylamides from flower-head derived in vitro callus cultures of Spilanthes paniculata", J Biosci Bioeng, 124, pp.99~107 (2017)
- Rasool A, "Medicinal plants : Role, distribution and future", J pharmacogn phytochem, 9, pp.2111~2114 (2020)
- Schultz F, "Pharmacological assessment of the antiprotozoal activity, cytotoxicity and genotoxicity of medicinal plants used in the treatment of Malaria in the greater Mpigi Region in Uganda", Front Pharmacol, 12, pp.678535~ (2021)
- Snow RW, "The prevalence of Plasmodium falciparum in sub-Saharan Africa since 1900", Nature, 550, pp.515~518 (2017)
- Srivastava A, "New look to phytomedicine", pp.625~655 (2019), Academic Press
- Taha A, "Brine shrimp bioassay of ethanol extracts of Sesuvium verrucosum, Salsola baryosma and Zygophyllum quatarense medicinal plants from Bahrain", Phytother Res, 14, pp.48~50 (2000)
- Touret F, "In vitro screening of a FDA approved chemical library reveals potential inhibitors of SARS-CoV-2 replication", Sci Rep, 10, pp.13093~ (2020)
- Valenzuela-Atondo DA, "Antioxidant activity of in vitro plantlets and callus cultures of Randia echinocarpa, a medicinal plant from northwestern Mexico", In Vitro Cell Dev Biol, 56, pp.440~446 (2020)
- Vats S, "Evaluation of phytochemicals, antioxidant and antimicrobial activity of in vitro culture of Vigna unguiculata (L.) walp", Researcher, 4, pp.70~74 (2012)
- Waako P J, "In vitro interactions of Aspilia africana (Pers.) CD Adams, a traditional antimalarial medicinal plant, with artemisinin against Plasmodium falciparum", Afr J Ecol, 102, pp.262~268 (2005)
- Waako P, "East African medicinal plants as a source of lead compounds for the development of new antimalarial drugs", J Ethnopharmacol, 45, pp.102~106 (2007)
- treatment, "Plos one", 2021, e0254804~
- Were P, "Prophylactic and curative activities of extracts from Warburgia ugandensis Sprague (Canellaceae) and Zanthoxylum usambarense (Engl.) Kokwaro (Rutaceae) against Plasmodium knowlesi and Plasmodium berghei", J Ethnopharmacol, 130, pp.158~162 (2010)
- Were PS, "Warburgia ugandensis : A potent in vivo phytomedicine against Plasmodium knowlesi", J Pharmacogn Phytochem, 9, pp.01~05 (2020)
- Wiedemair V, "Investigations into the total antioxidant capacities of cultivars of gluten-free grains using near-infrared spectroscopy", Food Control, 95, pp.189~195 (2019)
- WHO expert consultation on rabies : third report, "WHO(World Health Organization)", World Health Organization, ~ (2018)
- World malaria report 2022, "WHO(World Health Organization)", World Health Organization, ~ (2022)
- Zhang Z, "Selectivity of dietary phenolics for inhibition of human monoamine oxidases A and B", Biomed Res Int, 2019 (2019)
