Development and Validation of Simultaneous UV Spectrophotometric Method for the Determination of Levofloxacin and Ambroxol in Tablets
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
An accurate, specific and precise UV spectrophotometric method was developed for the simultaneous determination of levofloxacin (LFX) and ambroxol (ABX) in pharmaceutical dosage forms. The method involves formation of Q-absorbance equation at 219 (isoabsorptive point) and at 287 nm, using distilled water as a solvent. The linearity for both levofloxacin and ambroxol was in the range of 2-20 μg/ml and 5-50 μg/ml respectively. The % recovery was found to be 100-101% and 99-102% for levofloxacin and ambroxol respectively indicating proposed method is accurate and precise for simultaneous estimation of levofloxacin and ambroxol in tablets.
Abstract
An accurate, specific and precise UV spectrophotometric method was developed for the simultaneous determination of levofloxacin (LFX) and ambroxol (ABX) in pharmaceutical dosage forms. The method involves formation of Q-absorbance equation at 219 (isoabsorptive point) and at 287 nm, using distilled water as a solvent. The linearity for both levofloxacin and ambroxol was in the range of 2-20 μg/ml and 5-50 μg/ml respectively. The % recovery was found to be 100-101% and 99-102% for levofloxacin and ambroxol respectively indicating proposed method is accurate and precise for simultaneous estimation of levofloxacin and ambroxol in tablets.
참고문헌 (35)
- Sweetman, S. C, "Martindale The Complete Drug Reference; 33rd ed", pp.219~ (2002), Pharmaceutical Press
- Bertino, J, , Clin. Ther, 22, pp.798~ (2000)
- Lorian, V, "Antibiotics in Laboratory Medicine; 4th ed", pp.591~ (1996)
- Hardman, J, "The Pharmacological Basis of Therapeutics; 9th ed", pp.1065~ (1996), McGraw-Hill
- Budavari, S, "The Merck Index; 12th ed", pp.404~ (1996), Merck and Co. Inc
- Belal, F, , Talanta, 50, pp.765~ (1999)
- Ines, M, , J. Pharm. Biomed. Anal, 40, pp.179~ (2006)
- Neckle, U, , Anal. Chim. Acta, 463, pp.199~ (2002)
- Wong, F. A, , J. Pharm. Biomed. Anal, 15, pp.765~ (1997)
- Siewert, S, , J. Pharm. Biomed. Anal, 41, pp.1360~ (2006)
- Chepurwar, S. B, , J. Chromatogr. Sci, 45, pp.531~ (2007)
- Altiokka, G, , J. Pharm. Biomed. Anal, 30, pp.881~ (2002)
- Pospíilová, M, , J. Pharm. Biomed. Anal, 24, pp.421~ (2001)
- Perez-Ruiz, T, , J. Chromatogr. B, 742, pp.205~ (2000)
- Pérez-Ruiz, T, , J. Chromatogr. B, 692, pp.199~ (1997)
- Reddy, N. M, , Indian J. Pharm. Sci, 60, pp.249~ (1998)
- Colombo, L, , J. Chromatogr. B, 530, pp.141~ (1990)
- Schmid, J. J, , Chromatogr, 414, pp.65~ (1987)
- Bazylak, G, , J. Pharm. Biomed. Anal, 32, pp.887~ (2003)
- Kim, H, , J. Pharm. Biomed. Anal, 32, pp.209~ (2003)
- Heinänen, M, , J. Pharm. Biomed. Anal, 24, pp.1005~ (2001)
- Koundourellis, J. E, , J. Pharm. Biomed. Anal, 23, pp.469~ (2000)
- Nobilis, M, , J. Chromatogr, 581, pp.251~ (1992)
- Brizzi, V, , J. Pharm. Biomed. Anal, 8, pp.107~ (1990)
- Indrayanto, G, , J. Pharm. Biomed. Anal, 11, pp.781~ (1993)
- Hwang, M. S, , J. Pharm. Biomed. Anal, 8, pp.210~ (2005)
- Qi, M, , J. Pharm. Biomed. Anal, 35, pp.1287~ (2004)
- Dinçer, Z, , J. Pharm. Biomed. Anal, 5, pp.867~ (2003)
- Kothekar, K. M, , Eurasian J. Anal. Chem, 2, pp.21~ (2007)
- ICH, "International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use", ICH Harmonised Tripartite Guideline (1996)
- Ermer, J, , J. Pharm. Biomed. Anal, 37, pp.859~ (2005)
- Fabre, H, , LC-GC, 14, pp.302~ (2001)
- Braggio, S, , J. Pharm. Biomed. Anal, 14, pp.375~ (1996)
- Beckett, A. H, "Practical Pharmaceutical Chemistry; 4th ed", pp.275~ (1997), New Delhi
- Busaranon, K, , J. Pharm. Biomed. Anal, 41, pp.158~ (2006)