흑염소 간-조직과 Ferrocene 매개체를 사용한 과산화수소정량 전류법 바이오센서

Abstract

The response characteristics of the bioelectrode developed by the co-immobilization of black goat livertisue and ferocene in a carbon paste matrix for the amperometric determination of hydrogen peroxide were evaluated.In the range of electrode potential examined ( 0.3~+0.0 V vs. Ag/AgCl), the response time was relatively short (t95%=12s) and it responded in the wide range of pH. The detection limit was 2.25 106 M (S/N=3) and a relative standard devi-ation of the measurements which were repeated 15 times using 1.0 102 M hydrogen peroxide was 1.87%. The bio-electrode sensitivity decreased to 50% of the original value in 19 days of continuous use.