Effect of caspases and RANKL induced by heavy force in orthodontic root resorption
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objective: Orthodontic root resorption (ORR) due to orthodontic tooth movement is a difficult treatment-related adverse event. Caspases are important effector molecules for apoptosis. At present, little is known about the mechanisms underlying ORR and apoptosis in the cementum. The aim of the present in vivo study was to investigate the expression of tartrate-resistant acid phosphatase (TRAP), caspase 3, caspase 8, and receptor activator of nuclear factor kappa-B ligand (RANKL) in the cementum in response to a heavy or an optimum orthodontic force. Methods: The maxillary molars of male Wistar rats were subjected to an orthodontic force of 10 g or 50 g using a closed coil spring. The rats were sacrificed each experimental period on days 1, 3, 5, and 7 after orthodontic force application. And the rats were subjected to histopathological and immunohistochemical analyses. Results: On day 7 for the 50-g group, hematoxylin and eosin staining revealed numerous root resorption lacunae with odontoclasts on the root, while immunohistochemistry showed increased TRAP- and RANKL-positive cells. Caspase 3- and caspase 8-positive cells were increased on the cementum surfaces in the 50-g group on days 3 and 5. Moreover, the number of caspase 3- and caspase 8-positive cells and RANKL-positive cells was significantly higher in the 50-g group than in the 10-g group. Conclusions: In our rat model, ORR occurred after apoptosis was induced in the cementum by a heavy orthodontic force. These findings suggest that apoptosis of cementoblasts is involved in ORR.[Korean J Orthod 2018;48(4):253-261]
Abstract
Objective: Orthodontic root resorption (ORR) due to orthodontic tooth movement is a difficult treatment-related adverse event. Caspases are important effector molecules for apoptosis. At present, little is known about the mechanisms underlying ORR and apoptosis in the cementum. The aim of the present in vivo study was to investigate the expression of tartrate-resistant acid phosphatase (TRAP), caspase 3, caspase 8, and receptor activator of nuclear factor kappa-B ligand (RANKL) in the cementum in response to a heavy or an optimum orthodontic force. Methods: The maxillary molars of male Wistar rats were subjected to an orthodontic force of 10 g or 50 g using a closed coil spring. The rats were sacrificed each experimental period on days 1, 3, 5, and 7 after orthodontic force application. And the rats were subjected to histopathological and immunohistochemical analyses. Results: On day 7 for the 50-g group, hematoxylin and eosin staining revealed numerous root resorption lacunae with odontoclasts on the root, while immunohistochemistry showed increased TRAP- and RANKL-positive cells. Caspase 3- and caspase 8-positive cells were increased on the cementum surfaces in the 50-g group on days 3 and 5. Moreover, the number of caspase 3- and caspase 8-positive cells and RANKL-positive cells was significantly higher in the 50-g group than in the 10-g group. Conclusions: In our rat model, ORR occurred after apoptosis was induced in the cementum by a heavy orthodontic force. These findings suggest that apoptosis of cementoblasts is involved in ORR.[Korean J Orthod 2018;48(4):253-261]
참고문헌 (28)
- Kaley J, "Factors related to root resorption in edgewise practice", Angle Orthod, 61, pp.125~132 (1991)
- Linge BO, "Apical root resorption in upper anterior teeth", Eur J Orthod, 5, pp.173~183 (1983)[DOI]
- Owman-Moll P, "The effects of a four-fold increased orthodontic force magnitudeon tooth movement and root resorptions. An intraindividual study in adolescents", Eur J Orthod, 18, pp.287~294 (1996)[DOI]
- Owman-Moll P, "Effects of a doubled orthodontic force magnitude on tooth movement and root resorptions. An inter-individual study in adolescents", Eur J Orthod, 18, pp.141~150 (1996)[DOI]
- Chan E, "Physical properties of root cementum: Part 5. Volumetric analysis of root resorption craters after application of light and heavy orthodontic forces", Am J Orthod Dentofacial Orthop, 127, pp.186~195 (2005)[DOI]
- Takuji Hikida, "Comparisons of orthodontic root resorption under heavy and jiggling reciprocating forces during experimental tooth movement in a rat model", 대한치과교정학회지, 46(4), pp.228~241 (2016), 대한치과교정학회
- Baud V, "Signal transduction by tumor necrosis factor and its relatives", Trends Cell Biol, 11, pp.372~377 (2001)[DOI]
- Hamaya M, "Cell death of osteocytes occurs in rat alveolar bone during experimental tooth movement", Calcif Tissue Int, 70, pp.117~126 (2002)[DOI]
- Rana MW, "Detection of apoptosis during orthodontic tooth movement in rats", Am J Orthod Dentofacial Orthop, 119, pp.516~521 (2001)[DOI]
- Wu CC, "Capsaicin induced cell cycle arrest and apoptosis in human esophagus epidermoid carcinoma CE 81T", VGH cells through the elevation of intracellular reactive oxygen species and Ca2+ productions and caspase-3 activation, 2006, pp.601, 71~82
- Diercke K, "Gene expression profile of compressed primary human cementoblasts before and after IL-<TEX>$1{\beta}$<", TEX> stimulation, 2014, pp.18, 1925~1939
- Nakano Y, "Expressions of RANKL", RANK and M-CSF, Eur J Orthod, pp.2011, 33[DOI]
- Hatai T, "Apoptosis of periodontal ligament cells induced by mechanical stress during tooth movement", Oral Dis, 7, pp.287~290 (2001)[DOI]
- Asano M, "IL-8 and MCP- 1 induced by excessive orthodontic force mediates odontoclastogenesis in periodontal tissues", Oral Dis, 17, pp.489~498 (2011)[DOI]
- Boatright KM, "A unified model for apical caspase activation", Mol Cell, 11, pp.529~541 (2003)[DOI]
- Mabuchi R, "Cell proliferation and cell death in periodontal ligaments during orthodontic tooth movement", J Periodontal Res, 37, pp.118~124 (2002)[DOI]
- Zheng L, "Role of autophagy in tumor necrosis factor-<TEX>${\alpha}$<", TEX>- induced apoptosis of osteoblast cells, 2017, pp.65, 1014~1020
- Mitsuhashi M, "Effects of HSP70 on the compression force- induced TNF-<TEX>${\alpha}$<", TEX> and RANKL expression in human periodontal ligament cells, 2011, pp.60, 187~194
- Matsumoto Y, "Proinflammatory mediators related to orthodonticallyinduced periapical root resorption in rat mandibular molars", Eur J Orthod, 39, pp.686~691 (2017)[DOI]
- Szymczyk KH, "Active caspase-3 is required for osteoclast differentiation", J Cell Physiol, 209, pp.836~844 (2006)[DOI]
- Kagayama M, "Confocal microscopy of cementocytes and their lacunae and canaliculi in rat molars", Anat Embryol (Berl), 195, pp.491~496 (1997)[DOI]
- Ayasaka N, "Differences in the transport systems bet- ween cementocytes and osteocytes in rats using microperoxidase as a tracer", Arch Oral Biol, 37, pp.363~369 (1992)[DOI]
- Sasano Y, "Distinctive expression of extra- cellular matrix molecules at mRNA and protein levels during formation of cellular and acellular cementum in the rat", Histochem J, 33, pp.91~99 (2001)[DOI]
- Matsuzawa H, "Cementocyte cell death occurs in rat cellular cementum during orthodontic tooth movement", Angle Orthod, 87, pp.416~422 (2017)[DOI]
- Brudvik P, "Root resorption beneath the main hyalinized zone", Eur J Orthod, 16, pp.249~263 (1994)[DOI]
- Kurol J, "Hyalinization and root resorption during early orthodontic tooth movement in adolescents", Angle Orthod, 68, pp.161~165 (1998)
- Kvam E, "Scanning electron microscopy of tissue changes on the pressure surface of human premolars following tooth movement", Scand J Dent Res, 80, pp.357~368 (1972)
- Rygh P, "Ultrastructural cellular reactions in pressure zones of rat molar periodontium incident to orthodontic tooth movement", Acta Odontol Scand, 30, pp.575~593 (1972)[DOI]