수종 치과 교정용 밴드 시멘트의 세포독성에관한 실험적 연구
In vitro cytotoxicity of four kinds orthodontic band cements
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초록
고정식 교정 치료시 교정용 밴드가 장착된 치아의 치주조직 손상은 접착된 치아에서 보다 자주 발생한다. 일반 치과치료에 사용되는 시멘트는 세포독성을 지닌 것으로 알려져 있으므로 치주조직과 직접 접촉하거나 구강 내에서 밴드 시멘트의 성분들이 용출되는 교정용 밴드 시멘트는 치주조직의 손상을 일으키는 원인이 될 수 있다. 그러므로 교정용 밴드 시멘트의 생체 친화성은 재료의 물성과 함께 중요하게 고려되어야 할 사항이다. 이 연구의 목적은 교정용 밴드 시멘트의 세포독성을 평가하는 것으로 현재 임상에서 널리 사용되고 있는 zinc phosphate cement (ZPC), glass ionomer (GI), resin modified glass ionomer (RMGI), compomer의 생체 친화성을 평가하기 위해 human gingiva fibroblast를 배양하고 이 세포에 대한 세포수 산정 및 세포 형태관찰, MTT(3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, 한천중층시험 등의 세포독성 검사를 시행하여 다음과 같은 결과를 얻었다. 1. 세포수 산정 및 형태관찰 시험에서 GI > ZPC, RMGI, RMGI24, GI24 > compomer24, ZPC24, compomer 순으로 세포독성이 높게 나타났다. 2. MTT assay 에서는 GI > ZPC, RMGI > GI24 > ZPC24, compomer, RMGI24, compomer24 순으로 세포독성이 높게 나타났다. 3. 한천중층시험에서는 GI > GI24, ZPC, ZPC24, RMGI > RMGI24, compomer, compomer24 순으로 세포독성이 높게 나타났다.
Abstract
Orthodontic band cements are widely used in the fields of orthodontics, but they are commonly known as cytotoxic material. Within an oral cavity several ions and components are released from orthodontic band cements, thus causing inflammation or injury to the periodontal tissue. Therefore, it is very important to estimate the biocompatibility of orthodontic band cements. The purpose of this study was to assess the cytotoxic effect of orthodontic band cements to HGF cells. A zinc phosphate cement, a glass ionomer, a resin modified glass ionomer, and compomer were used to evaluate three cytotoxicity assays: cell proliferation assay, MTT assay, and agar ovelay assay. The results were as follows: 1. In the cell proliferation assay, GI > ZPC, RMGI, RMGI24, GI24 > compomer24, ZPC24, compomer> metal ring lined up in order of cytotoxicity. 2. In the MTT assay, GI > ZPC, RMGI > GI24> ZPC24, compomer, metal ring, RMGI24, compomer24 lined up in order of cytotoxicity. 3. In the agar overlay test, GI > GI24, ZPC, ZPC24, RMGI > RMGI24, compomer, compomer24, metal ring lined up in order of cytotoxicity.
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