Properties of Extremely Low Frequency Electromagnetic Fields and their Effects on Mouse Testicular Germ Cells
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초록
To evaluate the biohazard properties of an extremely low frequency electromagnetic field (ELF-EMF), we explored the physical properties of the ELF-EMF that generates the electric current induction in the secondary coil from the chamber of a primary solenoid coil. We subsequently explored the biological effects of a strong alternating electromagnetic field (EMF), ranging from 730-960 Gauss, on the mouse testis. Mice were exposed to an alternating EMF field induced by a rectangular electric current at 1, 7, 20, 40, and 80 Hertz, for 1, 3, 5, and 7 hours. The mouse testes were examined for proliferative activity and apoptosis using the in situ terminal deoxynucleotidyl transferase (TdT) method and by immunostaining of proliferating cell nuclear antigen (PCNA), respectively. We found that the electric current induction increased in the 6-8 Hertz range, and that exposure to an ELF-EMF induced the apoptosis of mouse spermatocytes. In situ TdT staining was found to be most prominent in 7 Hertz group, and gradually reduced in the 20,40, and 80 Hertz groups. These data suggest that a strong EMF can induce reproductive cell death within a short time,and the harmful effects of the EMF are maximal at low frequency alternating EMFs.
Abstract
To evaluate the biohazard properties of an extremely low frequency electromagnetic field (ELF-EMF), we explored the physical properties of the ELF-EMF that generates the electric current induction in the secondary coil from the chamber of a primary solenoid coil. We subsequently explored the biological effects of a strong alternating electromagnetic field (EMF), ranging from 730-960 Gauss, on the mouse testis. Mice were exposed to an alternating EMF field induced by a rectangular electric current at 1, 7, 20, 40, and 80 Hertz, for 1, 3, 5, and 7 hours. The mouse testes were examined for proliferative activity and apoptosis using the in situ terminal deoxynucleotidyl transferase (TdT) method and by immunostaining of proliferating cell nuclear antigen (PCNA), respectively. We found that the electric current induction increased in the 6-8 Hertz range, and that exposure to an ELF-EMF induced the apoptosis of mouse spermatocytes. In situ TdT staining was found to be most prominent in 7 Hertz group, and gradually reduced in the 20,40, and 80 Hertz groups. These data suggest that a strong EMF can induce reproductive cell death within a short time,and the harmful effects of the EMF are maximal at low frequency alternating EMFs.
참고문헌 (49)
- Adey WR, "Frequency and power windowing in tissue interactions with weak electromagnetic fields", Proceedings of the Institute of Electrical and Electronics Engineers, 68, pp.119~125 (1980)
- Ayrapetyan SN, "Magnetic fields alter electrical properties of solutions and their physiological effects", Bioelectromagnetics, 15, pp.133~142 (1994)
- Bassett CA, "The development and application of pulsed electromagnetic fields (PEMFs) for ununited fractures and arthrodeses", Orthop Clin North Am, 15, pp.61~87 (1984)
- Bawin SM, "Ionic factors in release of 45Ca2+ from chicken cerebral tissue by electromagnetic fields", Proc Natl Acad Sci USA, 75, pp.6314~6318 (1978)
- Blackman CF, "Induction of calcium-ion efflux from brain tissue by radio-frequency radiation: effects of modulation frequency and field strength", Radio Science, 14, pp.93~98 (1979)
- Blackman CF, "Effects of ELF (1-120 Hz) and modulated (50 Hz) RF fields on the efflux of calcium ions from brain tissue in vitro", Bioelectromagnetics, 6, pp.1~11 (1985)
- Buemi M, "Cell proliferation", cell death balance in renal cell cultures after exposure to a static magnetic field, 2001, pp.87, 269~273
- Carratore RD, "Effect of magnetic fields on rodent monooxygenase enzymes", Bioelectromagnetics, 16, pp.324~329 (1995)
- Cavol AV, "Measurement and analysis of static magnetic fields that block action potentials in cultures neurons", Bioelectomagnetics, 16, pp.197~206 (1995)
- Chiang H, "Pulsed magnetic field from video display terminals enhances teratogenic effects of cytosine arabinoside in mice", Bioelectromagnetics, 16, pp.70~74 (1995)
- Clermont Y, "Kinetics of spermatogenesis in mammals: Seminiferous epithelium cycle and spermatogonial renewal", Physiol Rev, 52, pp.198~236 (1972)
- De Kretser DM, "The cytology of testis" (1988), Raven Press
- De Vita R, "Effect of 50 Hz magnetic fields on mouse spermatogenesis monitored by flow cytometric analysis", Bioelectromagnetics, 16, pp.330~334 (1995)
- Diniz P, "Effects of pulsed electromagnetic field (PEMF) stimulation on bone tissue like formation are dependent on the maturation stages of the osteoblasts", Bioelectromagnetics, 23, pp.398~405 (2002)
- Engstroem S, "Dynamic properties of Lednev's parametric resonance mechanism", Bioelectromagnetics, 17, pp.58~70 (1996)
- Gluck B, "Inhibition of proliferation of human lymphoma cells U937 by a 50 Hz electromagnetic field", Cell Mol Biol, 47, pp.OL115~117 (2001)
- Grant G, "Protection against focal cerebral ischemia following exposure to a pulsed electromagnetic field", Bioelectromagnetics, 15, pp.205~216 (1994)
- Grota LJ, "Electric field exposure alters serum melatonin but not pineal melatonin synthesis in male rats", Bioelectromagnetics, 15, pp.4237~ (1995)
- Guzelsu N, "Effect of electromagnetic stimulation with different waveforms on cultured chick tendon fibroblasts", Bioelectromagnetics, 15, pp.115~131 (1994)
- Inoue N, "Effect of pulsed electromagnetic fields (PEMF) on late-phase osteotomy gap healing in a canine tibial model", J Orthop Res, 20, pp.1106~1114 (2002)
- Johnson L, "Efficiency of spermatogenesis", Microscopy Research and Technique, 32, pp.385~422 (1995)
- Johnson MT, "Electromagnetic fields used clinically to improve bone healing also impact lymphocyte proliferation in vitro", Biomed Sci Instrum, 37, pp.215~220 (2001)
- Kirschvink JL, "Magnetite in human tissues: a mechanism for the biological effects of weak ELF magnetic fields", Bioelectromagnetics, 1(Suppl), pp.101~113 (1992)
- 고성희, "The Effects of Mechanical Strain on Bone Cell Proliferation and Recruitment Induced by Osteocytes", International Journal of Oral Biology, 33(4), pp.179~186 (2008), 대한구강생물학회
- Kolosova LI, "Effect of low-intensity millimeter wave electromagnetic radiation on regeneration of the sciatic nerve in rats", Bioelectromagnetics, 17, pp.44~47 (1996)
- Lee JM, "Melatonin and puberty in female lambs exposed to EMF: A replicate study", Bioelectromagnetics, 16, pp.119~123 (1995)
- Lee SK, "An effective role of pulsed magnetic field for bony decalcification", Kor J Pathol, 27, pp.125~133 (1993)
- Li DK, "Magnetic fields and miscarriage", Epidemiology, 13, pp.237~238 (2002)
- Lindstroem E, "Intracellular calcium oscillation in a T-cell line after exposure to extremely-low-frequency magnetic fields with variable frequencies and flux densities", Bioelectromagnetics, 16, pp.41~47 (1995)
- Malko JA, "Search for influence of 1.5 Tesla magnetic field on growth of yeast cells", Bioelectromagnetics, 15, pp.495~501 (1994)
- Margonato V, "Biologic effects of prolonged exposure to ELF electromagnetic fields in rats: II. 50 Hz magnetic fields", Bioelectromagnetics, 16, pp.343~355 (1995)
- Orr JL, "Exposure of baboons to combined 60 Hz electric and magnetic fields does not produce work stoppage or affect operant performance on a match-tosample task", Bioelectromagnetics, 3, pp.61~70 (1995)
- Panagopoulos D, "Mechanism for action of electromagnetic fields on cells", Biochem Biophys Res Commun, 298, pp.95~ (2002)
- Panagopoulos DJ, "A mechanism for action of oscillating electric fields on cells", Biochem Biophys Res Commun, 272, pp.634~640 (2000)
- Parafiniuk M, "Effect of constant magnetic field on the liver of guinea pig. Electron microscopic studies", Folia Histochem Cytobiol, 30, pp.119~123 (1992)
- Portet RT, "Development of young rats and rabbits exposed to strong electric field", Bioelectromagnetics, 9, pp.95~104 (1988)
- Robison JG, "Decreased DNA repair rates and protection from heat induced apoptosis mediated by electromagnetic field exposure", Bioelectromagnetics, 23, pp.106~112 (2002)
- Rogers WR, "Nonhuman primates will not respond to turn off strong 60 Hz electric fields", Bioelectromagnetics, 3, pp.48~60 (1995)
- Rogers WR, "Regularly scheduled, day-time, slow onset 60 Hz electric and magnetic field exposure does not depress serum melatonin concentration in nonhuman primates", Bioelectromagnetics, 3(Supplement), pp.111~118 (1995)
- Ruiz Gomez MJ, "25 Hz electromagnetic field exposure has no effect on cell cycle distribution and apoptosis in U-937 and HCA-2", 1cch cells, 2001, pp.53, 137~140
- Saunders RD, "Weak electric field interactions in the central nervous system", Health Phys, 83, pp.366~375 (2002)
- Savitz DA, "Residential magnetic fields, wire codes,and pregnancy outcome", Bioelectromagnetics, 15, pp.271~273 (1994)
- Sienkiewicz Z, "Effects of prenatal exposure to 50 Hz magnetic fields on development in mice: II. Postnatal development and behavior", Bioelectromagnetics, 15, pp.363~375 (1994)
- Smith A, "Seasonal changes in testicular structure and function in the blue fox (Alopex lagopus), as quantified by morphometric analysis and measurement of adenylate cyclase activity", Int J Androl, 9, pp.53~66 (1986)
- Smith AJ, "Seasonal changes in spermatogenesis in the blue fox (Alopex lagopus), quantified by DNA flow cytometry and measurement of soluble Mn2 -dependent adenylate cyclase activity", J Reprod Fertil, 72, pp.453~461 (1984)
- Spadinger I, "3T3 cell motility and morphology before, during, and after exposure to extremely-lowfrequency magnetic fields", Bioelectromagnetics, 16, pp.178~187 (1995)
- Svedenstael B-M, "Fetal loss in mice exposed to magnetic fields during early pregnancy", Bioelectromagnetics, 16, pp.284~289 (1995)
- Takano-Yamamoto T, "Effect of a pulsing electromagnetic field on demineralized bone-matrixinduced bone formation in a bony defect in the premaxilla of rats", J Dent Res, 71, pp.1920~1925 (1992)
- Tofani S, "Static and ELF magnetic fields induce tumor growth inhibition and apoptosis", Bioelectromagnetics, 22, pp.419~428 (2001)
