Effects of 835-MHz Radiofrequency Radiation on the Chromosomal DNA of Mouse Thymic Lymphoma L5178Y Tk+/- Cells
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Abstract
This study was focused on the risk assessment of whether radiofrequency electromagnetic fields generated by mobile phone is cytogenetically toxic or not. We conducted the effects of 835-MHz electromagnetic field (EMF) on DNA strand breaks in mouse thymic lymphoma L5178Y Tk±/- cells using alkaline comet assay. EMF frequency 835-MHz we chosen is one of the most popular communication frequency bands in Korean code-division multiple-access (CDMA) mobile phone system. The cells were exposed to 835-MHz EMF alone or 835-MHz EMF combined with cyclophosamide (CPA) or 4-nitroquinoline-1-oxide (4NQO) at specific absorption rate (SAR) of 4.0W kg-1 for 24 and 48 hrs. DNA damage expressed as tail moment was increased more than two-fold after exposure to 835-MHz EMF for 24 and 48 hr. In particular, CPA for 48 hr and 4NQO for 24 hr enhanced notably the tail moment to 9-fold and 16-fold in the presence of 835-MHz EMF, respectively, compared to each single treatment. From these results, it appears that exposure to CDMA-mobile phone radiation at 835-MHz frequency may potentiate DNA strand breaks of mouse thymic lymphoma L5178Y Tk±/- cells under the defined conditions of this study.
참고문헌 (17)
- Chang SK, "Genotoxicityevaluation of electromagnetic fields generated by835-MHz mobile phone frequency band" (2004), in press
- Gandhi OP, "Some present problems anda proposed experimental phantom for SAR compliancetesting of cellular telephones at 835 and 1900 MHz", 47, pp.1501~1508 (2002)
- Goswami PC, "ProtooncogenemRNA levels and activities of multiple transcriptionfactors in C3H 10T1", 2 murine embryonic fibroblastsexposed to 835, 1999, pp.151, 200-~309
- Hengstler JG, "Induction of DNA crosslinks and DNAstrand lesions by cyclophosphamide after activation bycytochrome P450 2B1", 373, pp.215~223 (1997)
- Hone P, "Possible associations between ELF electromagneticfieldsDNA damage response processes and childhoodleukemia", pp.1939-~1941 (2003)
- Ivancsits S,, "Induction of DNA strand breaks by intermittentexposure to extremely-low-frequency electromagneticfields in human diploid fibroblasts", 519:, pp.1~13 (2002)
- Lai H, "Single- and double-strandDNA breaks in rat brain cells after acute exposure toradiofrequency electromagnetic radiation", 69, pp.513~521 (1996)
- Lai H, "Melatonin and N-tert-butyl-α-phenylnitrone block 60-Hz magnetic field-inducedDNA single and double strand breaks in rat brain cells", 22, pp.152~162 (1997)
- Malyapa RS,, "Measurement of DNAdamage after exposure to electromagnetic radiation inthe cellular phone communication frequency band", 148, pp.618~627 (1997)
- Miyakoshi J,, "Exposure to strong magnetic fields at power frequencypotentiates X-ray-induced DNA strand breaks", 41, pp.293~302 (2000)
- Narita K,6 K Hanakawa,, "Induction of apoptotic cell death in humanleukemic cell line analysis of the possiblemechanisms in vitro", 11, pp.329~335 (1997)
- Roti Roti JL,, "Neoplastic transformationin C3H 10T1", 2 cells after exposure to 835, 2001, pp.155, 239~247
- Salford LG,, "Nerve cell damage in mammalian brainafter exposure to microwaves from GSM mobile phones", 111, pp.881~883 (2003)
- Tian F, "Exposure to 2 45 GHz electromagnetic fields induceshsp70 at a high SAR of more than 20Wkg-1 but notat 5Wkg-1 in human glioma MO54 cells", 78, pp.433~440 (2002)
- Tice R, "guidelinesfor in vito and in vivo genetic toxicology testing", 35, pp.206~221 (2000)
- Valentin-Severin I, "Use of HepG2 cell line fordirect or indirect mutagens screening comparativeinvestigation between comet and micronucleus assays", 536(t), pp.79~90 (2003)
- Zeni O, "Lack of genotoxic effects in human lymphocytes exposedin vitro to 900 MHz electromagnetic fields", 160:, pp.152~158 (2003)