Intracisternal Administration of Voltage Dependent Calcium Channel Blockers Attenuates Orofacial Inflammatory Nociceptive Behavior in Rats
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Voltage dependent calcium channel (VDCC), one of the most important regulator of Ca 2+concentration in neuron,play an essential role in the central processing of nociceptive information. The present study investigated the antinociceptive effects of L, T or N type VDCC blockers on the formalin-induced orofacial inflammatory pain. Experiments were carried out on adult male Sprague-Dawley rats weighing 220-280 g. Anesthetized rats were individually fixed on a stereotaxic frame and a polyethylene (PE) tube was implanted for intracisternal injection. After 72 hours, 5%formalin (50 μL) was applied subcutaneously to the vibrissa pad and nociceptive scratching behavior was recorded for nine successive 5 min intervals. VDCC blockers were administered intracisternally 20 minutes prior to subcutaneous injection of formalin into the orofacial area. The intracisternal administration of 350 or 700 μg of verapamil, a blocker of L type VDCC, significantly decreased the number of scratches and duration in the behavioral responses produced by formalin injection. Intracisternal administration of 75 or 150 μg of mibefradil, a T type VDCC blocker, or 11 or 22 μg of cilnidipine, a N type VDCC blocker, also produced significant suppression of the number of scratches and duration of scratching in the first and second phase. Neither intracisternal administration of all VDCC blockers nor vehicle did not affect in motor dysfunction. The present results suggest that central VDCCs play an important role in orofacial nociceptive transmission and a targeted inhibition of the VDCCs is a potentially important treatment approach for inflammatory pain originating in the orofacial area.
Abstract
Voltage dependent calcium channel (VDCC), one of the most important regulator of Ca 2+concentration in neuron,play an essential role in the central processing of nociceptive information. The present study investigated the antinociceptive effects of L, T or N type VDCC blockers on the formalin-induced orofacial inflammatory pain. Experiments were carried out on adult male Sprague-Dawley rats weighing 220-280 g. Anesthetized rats were individually fixed on a stereotaxic frame and a polyethylene (PE) tube was implanted for intracisternal injection. After 72 hours, 5%formalin (50 μL) was applied subcutaneously to the vibrissa pad and nociceptive scratching behavior was recorded for nine successive 5 min intervals. VDCC blockers were administered intracisternally 20 minutes prior to subcutaneous injection of formalin into the orofacial area. The intracisternal administration of 350 or 700 μg of verapamil, a blocker of L type VDCC, significantly decreased the number of scratches and duration in the behavioral responses produced by formalin injection. Intracisternal administration of 75 or 150 μg of mibefradil, a T type VDCC blocker, or 11 or 22 μg of cilnidipine, a N type VDCC blocker, also produced significant suppression of the number of scratches and duration of scratching in the first and second phase. Neither intracisternal administration of all VDCC blockers nor vehicle did not affect in motor dysfunction. The present results suggest that central VDCCs play an important role in orofacial nociceptive transmission and a targeted inhibition of the VDCCs is a potentially important treatment approach for inflammatory pain originating in the orofacial area.
참고문헌 (56)
- Abbott FV, "The formalin test:scoring properties of the first and second phases of the pain response in rats", Pain, 60, pp.91~102 (1995)
- Ahlijanian MK, "Subunit structure and localization of dihydropyridine-sensitive calcium channels in mammalian brain, spinal cord and retina", Neuron, 4, pp.819~832 (1990)
- Ahn DK, "Intracisternal administration of chemokines facilitated formalin-induced behavioral responses in the orofacial area of freely moving rats", Brain Res Bull, 66, pp.50~58 (2005)
- Ahn DK, "Central NO is involved in the antinociceptive action of intracisternal antidepressants in freely moving rats", Neurosci Lett, 243, pp.105~108 (1998)
- Altier C, "Targeting Ca2+ channels to treat pain: T-type versus N-type", Trends Pharmacol Sci, 25, pp.465~470 (2004)
- Barclay JW, "Calcium-dependent regulation of exocytosis", Cell Calcium, 38, pp.343~353 (2005)
- Barton ME, "The antihyperalgesic effects of the T-type calcium channel blockers ethosuximide, trimethadione, and mibefradil", Eur J Pharmacol, 521, pp.79~85 (2005)
- Carbone E, "A low voltage-activated, fully inactivating Ca channel in vertebrate sensory neurones", Nature, 310, pp.501~502 (1984)
- Chen JQ, "Analgesic effect of huwentoxin-I, a new N-type voltagesensitive calcium channel blocker, on acute visceral pain in rats", Di Yi Jun Yi Da Xue Xue Bao, 25, pp.10~14 (2005)
- Cheng JK, "Effects of intrathecal injection of T-type calcium channel blockers in the rat formalin test", Behav Pharmacol, 18, pp.1~8 (2007)
- Choi HS, "Effects of TNF-alpha injected intracisternally on the nociceptive jaw-opening reflex and orofacial formalin test in freely moving rats", Prog Neuropsychopharmacol Biol Psychiatry, 27, pp.613~618 (2003)
- Choi HS, "Effects of intracisternal injection of interleukin-6 on nociceptive jaw opening reflex and orofacial formalin test in freely moving rats", Brain Res Bull, 59, pp.365~370 (2003)
- Choi S, "Attenuated pain responses in mice lacking Ca(V)3.2 T-type channels", Genes Brain Behav, 6, pp.425~431 (2007)
- Christensen D, "Combined systemic administration of the glycine", NMDA receptor antagonist, (+)-HA966 and morphine attenuates pain-related behaviour in a rat model of trigeminal neuropathic pain, 1999, pp.83, 433~440
- Coderre TJ, "The role of NMDA receptor-operated calcium channels in persistent nociception after formalininduced tissue injury", J Neurosci, 12, pp.3671~3675 (1992)
- Diaz A, "Blockade of spinal N- and P-type, but not L-type, calcium channels inhibits the excitability of rat dorsal horn neurones produced by subcutaneous formalin inflammation", Pain, 69, pp.93~100 (1997)
- Dunham NW, "A note on a simple apparatus for detecting neurological deficit in rats and mice", J Am Pharm Assoc Am Pharm Assoc, 46, pp.208~209 (1957)
- Ebersberger A, "Effects of N-, P", Q- and L-type calcium channel blockers on nociceptive neurones of the trigeminal nucleus with input from the dura, 2004, pp.24, 250~261
- Zhi Fang, "R-type Calcium Channel Isoform in Rat Dorsal Root Ganglion Neurons", The Korean Journal of Physiology & Pharmacology, 14(1), pp.45~49 (2010), 대한약리학회
- Gohil K, "Neuroanatomical distribution of receptors for a novel voltagesensitive calcium channel antagonist, SNX-230 (q-conopeptide MVIIC)", Brain Res, 653, pp.258~266 (1994)
- Gribkoff VK, "The role of voltage-gated calcium channels in pain and nociception", Semin Cell Dev Biol, 17, pp.555~564 (2006)
- Gurdal H, "Effects of calcium channel blockers on formalin-induced nociception and inflammation in rats", Pharmacology, 44, pp.290~296 (1992)
- Hatakeyama S, "Differential nociceptive responses in mice lacking the alpha(1B) subunit of N-type Ca(2+) channels", Neuroreport, 12, pp.2423~2427 (2001)
- Heinke B, "Pre-and postsynaptic contributions of voltage-dependent Ca2+ channels to nociceptive transmission in rat spinal lamina I neurons", Eur J Neurosci, 19, pp.103~111 (2004)
- Hell JW, "Snutch TP and Catterall WA. Identification and differential subcellular localization of the neuronal class C and class D L-type calcium channel a1 subunits", J Cell Biol, 123, pp.949~962 (1993)
- Himpens B, "Ca2+ homeostasis in vascular smooth muscle. J Vasc Res. 1995;32:207-19. Idanpaan-Heikkila JJ, Guilbaud G. Pharmacological studies on a rat model of trigeminal neuropathic pain: baclofen, but not carbamazepine, morphine or tricyclic antidepressants, attenuates the allodynia-like behaviour", Pain, 79, pp.281~290 (1999)
- Ikeda H, "Synaptic plasticity in spinal lamina I projection neurons that mediate hyperalgesia", Science, 299, pp.1237~1240 (2003)
- Ikeda M, "Classification of voltage-dependent Ca2+ channels in trigeminal ganglion neurons from neonatal rats", Life Sci, 18, pp.1175~1187 (2003)
- Jiang Z, "Brownstone RM. Development of L-type calcium channels and a nifedipine-sensitive motor activity in the postnatal mouse spinal cord", Eur J Neurosci, 11, pp.3481~3487 (1999)
- Katoh A, "Motor deficits in homozygous and heterozygous p", q-type calcium channel mutants, 2007, pp.97, 1280~1287
- omega-Conotoxin in rat brain, "Eur J Pharmacol", 1988, 181~183
- Kim C, "Altered nociceptive response in mice deficient in the alpha(1B) subunit of the voltage-dependent calcium channel", Mol Cell Neurosci, 18, pp.235~245 (2001)
- Koganei H, "Suppression of formalin-induced nociception by cilnidipine, a voltage-dependent calcium channel blocker", Biol Pharm Bull, 32, pp.1695~1700 (2009)
- Lee MK, "Low doses of cannabinoids enhance the antinociceptive effects of intracisternally administered mGluRs groups II and III agonists in formalininduced TMJ nociception in rats", Pain, 139, pp.367~375 (2008)
- Levi AJ, "One hump or two? The triggering of calcium release from the sarcoplasmic reticulum and the voltage dependence of contraction in mammalian cardiac muscle", Cardiovasc Res, 27, pp.1743~1757 (1993)
- Liang YC, "The synthetic cannabinoids attenuate allodynia and hyperalgesia in a rat model of trigeminal neuropathic pain", Neuropharmacology, 53, pp.169~177 (2007)
- Lipscombe D, "L-type calcium channels: the low down", J Neurophysiol, 92, pp.2633~2641 (2004)
- Lux EA, "Case report: successful treatment of a patient with trigeminal neuropathy using ziconotide", Anesth Analg, 110, pp.1195~1197 (2010)
- Malmberg AB, "Voltage-sensitive calcium channels in spinal nociceptive processing: blockade of N- and P-type channels inhibits formalin-induced nociception", J Neurosci, 14, pp.4882~4890 (1994)
- Miller RJ, "Multiple calcium channels and neuronal function", Science, 235, pp.46~52 (1987)
- Mochida S, "Evidence for a voltage-dependent enhancement of neuro-transmitter release mediated via the ynaptic protein interaction site of N-type Ca2+ channels", Proc Natl Acad Sci U S A, 95, pp.14523~14528 (1998)
- Murakami M, "Antinociceptive effect of cilnidipine, a novel N-type calcium channel antagonist", Brain Res, 868, pp.123~127 (2000)
- Raboisson P, "The orofacial formalin test", Neurosci Biobehav Rev, 28, pp.219~226 (2004)
- Raboisson P, "Effects of subcutaneous formalin on the activity of trigeminal brain stem nociceptive neurones in the rat", J Neurophysiol, 73, pp.496~505 (1995)
- Rozas G, "Labandeira-Garcia JL. An automated rotarod method for quantitative drug-free evaluation of overall motor deficits in rat models of parkinsonism", 2, pp.75~84 (1997)
- Schmidtko A, "Geisslinger G. Ziconotide for treatment of severe chronic pain", Lancet, 375, pp.1569~1577 (2010)
- Sluka KA, "Blockade of N- and P", O-type calcium channels reduced the secondary heat hyperalgesia induced by acute inflammation, 1998, pp.287, 232~237
- Snutch TP, "Targeting chronic and neuropathic pain: the N-type calcium channel comes of age", NeuroRx, 2, pp.662~670 (2005)
- Stirling LC, "Nociceptor-specific gene deletion using heterozygous NaV1.8-Cre recombinase mice", Pain, 113, pp.27~36 (2005)
- Tjolsen A, "The formalin test: an evaluation of the method", Pain, 51, pp.5~17 (1992)
- Wang XM, "Effect of antisense knock-down of alpha(2a)- and alpha(2c)-adrenoceptors on the antinociceptive action of clonidine on trigeminal nociception in the rat", Pain, 98, pp.27~35 (2002)
- White G, "lowthreshold Ca2+ current triggers burst firing through an afterdepolarizing potential in an adult mammalian neuron", Proc Natl Acad Sci USA, 86, pp.6802~6806 (1989)
- Xu W, "Neuronal Ca(v)1.3alpha(1) L-type channels activate at relatively hyperpolarized membrane potentials and are incompletely inhibited by dihydropyridines", J Neurosci, 21, pp.5944~5951 (2001)
- Yaksh TL, "Chronic catheterization of the spinal subarachnoid space", Physiol Behav, 17, pp.1031~1036 (1976)
- Yang GY, "Intracisternal Administration of NR2 Antagonists Attenuates Facial Formalin-induced Nociceptive Behavior in Rats", JOURNAL OF OROFACIAL PAIN, 24(2), pp.203~211 (2010), QUINTESSENCE PUBLISHING CO INC
- Zheng X, "Asequential view of neurotransmitter release", Brain Res Bull, 47, pp.117~128 (1998)
