Differential Expression of Osteonectin in the Rat Developing Molars
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Tooth development involves bud, cap, bell and hard tissue formation stages, each of which is tightly controlled by regulatory molecules. The aim of this study was to identify genes that are differentially expressed during dental hard tissue differentiation. Sprague-Dawley rats at postnatal days 3, 6 and 9 were used in the analysis. Differential display RT-PCR (DD-PCR) was used to screen differentially expressed genes between the 2nd (root formation stage,during mineralization) and 3rd (cap stage, before mineralization)molar germs at postnatal day 9. The DNA detected in the 2nd molar germs showed homology to osteonectin only (GenBank accession no. NM_012656.1). The level of osteonectin mRNA expression was much higher in the 2nd molar germs than in the 3rd molar germs and was found to increase in a time-dependent manner from the early bell stage to the root formation stage in the 2nd molar germs. The pattern of osteonectin protein expression was consistent with these RT-PCR results. Osteonectin protein was found by immunofluorescent analysis to localize in odontoblasts and preodontoblasts rather than the dentin matrix itself. Further studies are needed to validate the involvement of osteonectin in mineralization and root formation.
Abstract
Tooth development involves bud, cap, bell and hard tissue formation stages, each of which is tightly controlled by regulatory molecules. The aim of this study was to identify genes that are differentially expressed during dental hard tissue differentiation. Sprague-Dawley rats at postnatal days 3, 6 and 9 were used in the analysis. Differential display RT-PCR (DD-PCR) was used to screen differentially expressed genes between the 2nd (root formation stage,during mineralization) and 3rd (cap stage, before mineralization)molar germs at postnatal day 9. The DNA detected in the 2nd molar germs showed homology to osteonectin only (GenBank accession no. NM_012656.1). The level of osteonectin mRNA expression was much higher in the 2nd molar germs than in the 3rd molar germs and was found to increase in a time-dependent manner from the early bell stage to the root formation stage in the 2nd molar germs. The pattern of osteonectin protein expression was consistent with these RT-PCR results. Osteonectin protein was found by immunofluorescent analysis to localize in odontoblasts and preodontoblasts rather than the dentin matrix itself. Further studies are needed to validate the involvement of osteonectin in mineralization and root formation.
참고문헌 (18)
- Thesleff I, "Molecular regulation of tooth development", Bone, 25, pp.123~125 (1999)
- Hansen L, "A novel nonsense mutation in PAX9 is associated with marked variability in number of missing teeth", Eur J Oral Sci, 115, pp.330~333 (2007)
- Schwab W, "Immunocytochemical and biochemical detection of EMMPRIN in the rat tooth germ: dfferentiation- dependent co-expression with MMPs and co-localization with caveolin-1 in membrane rafts of dental epithelial cells", Histochem Cell Biol, 128(3), pp.195~203 (2007)
- Tucker A, "The cutting-edge of mammalian development; how the embryo makes teeth", Nat Rev Genet, 5(7), pp.499~508 (2004)
- Jee-Hae Kang, "The Epithelial-Mesenchymal Transition During Tooth Root Development", International Journal of Oral Biology, 36(3), pp.135~141 (2011), 대한구강생물학회
- Pispa J, "Mechanism of ectodermal organogenesis", Dev Biol, 262, pp.195~205 (2003)
- Min-Seok Kim, "MMP-2 and MMP-9 are Differentially Involved in Molar Growth", International Journal of Oral Biology, 36(4), pp.195~201 (2011), 대한구강생물학회
- McVey J.H, "Characterization of the mouse PARC", osteonectin gene: intron, J Biol Chem, pp.1988, 263[DOI]
- Mason I.J, "Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial “culture shock” glycoprotein", MBO J, 5, pp.1465~1472 (1986)
- Sage E.H, "Characterization of a novel serum albumin-binding glycoprotein secreted by endothelial cells in culture", J Biol Chem, 259, pp.3993~4007 (1984)
- Mann K, "Solubilization of protein BM-40 from a basement membrane tumor with chelating agents and evidence for its identity with osteonectin and SPARC", FEBS, 218, pp.167~172 (1987)
- Maurer P, "The C-terminal portion of BM-40 (SPARC", osteonectin) is an autonomously folding and crystallisable domain that binds calcium and collagen IV, 1995, pp.253(2), 347~357
- Sasaki T, "Limited cleavage of extracellular matrix protein BM-40 by matrix metalloproteinases increases its affinity for collagens", J Biol Chem, 272, pp.9237~9243 (1997)
- Termine J.D, "Osteonectin, a bone-specific protein linking mineral to collagen", Cell, 26, pp.99~105 (1981)
- Motamed K, "Fibroblast growth factor receptor-1 mediates the inhibition of endothelial cell proliferation and the promotion of skeletal myoblast differentiation by SPARC: a role for protein kinase A", J Cell Biochem, 90(2), pp.408~423 (2003)
- Lane TF, "The biology of SPARC, a protein that modulates cell-matrix interactions", FASEB J, 8(2), pp.163~173 (1994)
- Sodek J, "Novel functions of the matricellular proteins osteopontin and osteonectin", SPARC, 2002, pp.43(2), 308~319
- Trombetta JM, "SPARC", osteonectin functions to maintain homeostasis of the collagenous extracellular matrix in the periodontal ligament, 2010, pp.58(10), 871~879