Molecular Identification of Bacterial Species Present on Toothbrushes
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Toothbrushes play an essential role in oral hygiene. However, they can be significant in microbial transmissionand can increase the risk of infection, since they can serve asa reservoir for microorganisms in healthy, oral-diseased andmedically ill adults. This study was conducted to evaluatetoothbrush contamination in six toothbrushes donated fromfour people. Two participants each supplied two toothbrushes– one used in the bathroom and one used in the workplace. Theother two people each donated two toothbrushes used in theworkplace. Polymerase chain reaction was used to construct a16S rRNA clone library. Sequences of cloned DNA werecompared with those from the reference organisms providedby GenBank. A total 120 clones, representing 20 clones foreach toothbrush, were analyzed. They are composed of sixpylum, 46 genera and 79 species. The most dominant specieswere Streptococcus oralis, Streptococcus parasanguinis andHaemophilus parainfluenzae. Enterobacter and Escherichiawere recovered from toothbrushes used domestically. Toothbrushes used in the workplace did not contain Enterobacteria.
Abstract
Toothbrushes play an essential role in oral hygiene. However, they can be significant in microbial transmissionand can increase the risk of infection, since they can serve asa reservoir for microorganisms in healthy, oral-diseased andmedically ill adults. This study was conducted to evaluatetoothbrush contamination in six toothbrushes donated fromfour people. Two participants each supplied two toothbrushes– one used in the bathroom and one used in the workplace. Theother two people each donated two toothbrushes used in theworkplace. Polymerase chain reaction was used to construct a16S rRNA clone library. Sequences of cloned DNA werecompared with those from the reference organisms providedby GenBank. A total 120 clones, representing 20 clones foreach toothbrush, were analyzed. They are composed of sixpylum, 46 genera and 79 species. The most dominant specieswere Streptococcus oralis, Streptococcus parasanguinis andHaemophilus parainfluenzae. Enterobacter and Escherichiawere recovered from toothbrushes used domestically. Toothbrushes used in the workplace did not contain Enterobacteria.
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