Malassezia spp.의 최적 성장을 위한 Modified BHI Medium의 조성
The Composition of Modified BHI Medium for Optimal Growth of Malassezia spp.
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Background: There are several media for culture of Malassezia spp., such as Leeming & Notman(LN) medium, modified Leeming & Notman (mLN) medium, Dixon's medium and modified Dixon'smedium etc. It is known that Malassezia spp. grow well in these media in general, but the kind andamounts of their ingredients are various and un-uniform according to researchers. Author propose thenew and transparent BHI based medium for the optimal growth of Malassezia spp. Objectives: The purpose of this study was to design the simple and transparent BHI based mediumand find essential ingredients for the growth of M. globosa and M. obtusa. Methods: The colony size of eight standard strains (M. dermatis, M. furfur, M. globosa, M. japonica,M. obtusa, M. sloofiae, M. sympodialis, M. yamatoensis) on the modified BHI (mBHI) agar media withdifferent ingredients was observed by naked eye after seven day culture. The compositions of mBHImedium were as follows; mBHI-1 was supplemented with 0.7% dextrose, 1.5% Tween 80, 1% glycerolto BHI medium, mBHI-2 was supplemented with 1.5% Tween 40 to mBHI-1 instead of Tween 80,mBHI-3 was supplemented with 1.5% Tween 60 to mBHI-1 instead of Tween 80, mBHI-4 was addedwith 0.8% bile salts to mBHI-1. mBHI-5 was supplemented with 1.5% Tween 60 to mBHI-4 instead ofTween 80, and mBHI-6 was supplemented with 1.5% Tween 40 to mBHI-4 instead of Tween 80. pH ofsix mBHI media was all adjusted to 6.5. Results: M. furfur & M. japonica were grown well on mBHI-1 agar, but M. globosa & M. obtusawere not grown and others grown poorly. M. globosa & M. obtusa were not grown on mBHI-1 &mBHI-4 containing Tween 80 as lipid source, but others grown on all mBHI media. The media that alleight Malassezia strains grew well were slightly turbid mBHI-5 & transparent mBHI-6 medium. Conclusions: M. globosa & M. obtusa need glycerol and bile salts as well as Tween 60 or 40 insteadof Tween 80 for growth. M. furfur & M japonica need not bile salts for growth. Author proposes the transparent modified BHI medium supplemented with 0.7% dextrose, 1.5% Tween 40, 1% glycerol and0.8% bile salts (mBHI-6) as new standard medium for culture of eight Malassezia species.
Abstract
Background: There are several media for culture of Malassezia spp., such as Leeming & Notman(LN) medium, modified Leeming & Notman (mLN) medium, Dixon's medium and modified Dixon'smedium etc. It is known that Malassezia spp. grow well in these media in general, but the kind andamounts of their ingredients are various and un-uniform according to researchers. Author propose thenew and transparent BHI based medium for the optimal growth of Malassezia spp. Objectives: The purpose of this study was to design the simple and transparent BHI based mediumand find essential ingredients for the growth of M. globosa and M. obtusa. Methods: The colony size of eight standard strains (M. dermatis, M. furfur, M. globosa, M. japonica,M. obtusa, M. sloofiae, M. sympodialis, M. yamatoensis) on the modified BHI (mBHI) agar media withdifferent ingredients was observed by naked eye after seven day culture. The compositions of mBHImedium were as follows; mBHI-1 was supplemented with 0.7% dextrose, 1.5% Tween 80, 1% glycerolto BHI medium, mBHI-2 was supplemented with 1.5% Tween 40 to mBHI-1 instead of Tween 80,mBHI-3 was supplemented with 1.5% Tween 60 to mBHI-1 instead of Tween 80, mBHI-4 was addedwith 0.8% bile salts to mBHI-1. mBHI-5 was supplemented with 1.5% Tween 60 to mBHI-4 instead ofTween 80, and mBHI-6 was supplemented with 1.5% Tween 40 to mBHI-4 instead of Tween 80. pH ofsix mBHI media was all adjusted to 6.5. Results: M. furfur & M. japonica were grown well on mBHI-1 agar, but M. globosa & M. obtusawere not grown and others grown poorly. M. globosa & M. obtusa were not grown on mBHI-1 &mBHI-4 containing Tween 80 as lipid source, but others grown on all mBHI media. The media that alleight Malassezia strains grew well were slightly turbid mBHI-5 & transparent mBHI-6 medium. Conclusions: M. globosa & M. obtusa need glycerol and bile salts as well as Tween 60 or 40 insteadof Tween 80 for growth. M. furfur & M japonica need not bile salts for growth. Author proposes the transparent modified BHI medium supplemented with 0.7% dextrose, 1.5% Tween 40, 1% glycerol and0.8% bile salts (mBHI-6) as new standard medium for culture of eight Malassezia species.
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