Increased SOX2 expression in three-dimensional sphere culture of dental pulp stem cells
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Mesenchymal stem cells in the dental pulp exhibit a tendency for differentiation into various dental lineages and holdgreat potential as a major conduit for regenerative treatment in dentistry. Although they can be readily isolated fromteeth, the exact characteristics of these stem cells have not been fully understood so far. When compared to twodimensional(2D) cultures, three-dimensional (3D) cultures have the advantage of enriching the stem cell population. Hence, 3D-organoid culture and 3D-sphere culture were applied to dental pulp cells in the current study. Althoughthe establishment of the organoid culture proved unsuccessful, the 3D-sphere culture readily initiated the stablegeneration of cell aggregates, which continued to grow and could be passaged to the second round. Interestingly, asignificant increase in SOX2 expression was detected in the 3D-spheroid culture compared to the 2D culture. Theseresults indicate the enrichment of the stemness-high population in the 3D-sphere culture. Thus, 3D-sphere culturemay act as a link between the conventional and 3D-organoid cultures and aid in understanding the characteristics ofdental pulp stem cells.
Abstract
Mesenchymal stem cells in the dental pulp exhibit a tendency for differentiation into various dental lineages and holdgreat potential as a major conduit for regenerative treatment in dentistry. Although they can be readily isolated fromteeth, the exact characteristics of these stem cells have not been fully understood so far. When compared to twodimensional(2D) cultures, three-dimensional (3D) cultures have the advantage of enriching the stem cell population. Hence, 3D-organoid culture and 3D-sphere culture were applied to dental pulp cells in the current study. Althoughthe establishment of the organoid culture proved unsuccessful, the 3D-sphere culture readily initiated the stablegeneration of cell aggregates, which continued to grow and could be passaged to the second round. Interestingly, asignificant increase in SOX2 expression was detected in the 3D-spheroid culture compared to the 2D culture. Theseresults indicate the enrichment of the stemness-high population in the 3D-sphere culture. Thus, 3D-sphere culturemay act as a link between the conventional and 3D-organoid cultures and aid in understanding the characteristics ofdental pulp stem cells.
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