OPB, a water extract from Rehmannia glutinosa Libosch and Eleutherococcus senticosus Max, inhibits osteoclast differentiation and function
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We performed the present study to investigate whether Rehmannia glutinosa Libosch (RG) extracts (RGX) and Eleutherococcus senticosus Max (ES) extracts (ESX) play any roles in bone metabolism. We examined cellular activities of bone cells by measurement of osteoblastic cell viability, osteoprotegerin (OPG) secretion from steoblasts,osteoclastogenesis, and osteoclastic activity. There is no cytotoxicity from osteoblasts after treatment with RGX and ESX. The secretion of OPG from the osteoblasts showed marked increases after treatment with RGX and ESX. In addition, RGX and ESX treatment decreased the number of tartrate-resistant acid phosphatase-positive multinucleated cells and the resorption areas. RGX and ESX, when mixed at optimal ratios, induced synergic effects, in vitro. OPB,which showed synergic effects, is the extract of natural ingredients RG and ES mixed at a raw material weight ratio of 4 : 1. It can be suspected that extracts of RG and ES mixtures contains active ingredients involved in bone tissue metabolism and may be effective in improving osteoporosis.
Abstract
We performed the present study to investigate whether Rehmannia glutinosa Libosch (RG) extracts (RGX) and Eleutherococcus senticosus Max (ES) extracts (ESX) play any roles in bone metabolism. We examined cellular activities of bone cells by measurement of osteoblastic cell viability, osteoprotegerin (OPG) secretion from steoblasts,osteoclastogenesis, and osteoclastic activity. There is no cytotoxicity from osteoblasts after treatment with RGX and ESX. The secretion of OPG from the osteoblasts showed marked increases after treatment with RGX and ESX. In addition, RGX and ESX treatment decreased the number of tartrate-resistant acid phosphatase-positive multinucleated cells and the resorption areas. RGX and ESX, when mixed at optimal ratios, induced synergic effects, in vitro. OPB,which showed synergic effects, is the extract of natural ingredients RG and ES mixed at a raw material weight ratio of 4 : 1. It can be suspected that extracts of RG and ES mixtures contains active ingredients involved in bone tissue metabolism and may be effective in improving osteoporosis.
참고문헌 (43)
- Awang, D.V.C, "Siberian ginseng toxicity may be a case of mistaken identity", 155, pp.1237~ (1996)
- Baron, "Kinetic and cytochemical identification of osteoclast precursors and their differentiation into multinucleated osteoclasts", pp.363~378 (1986)
- Chin, "Effect of Panax ginseng saponins and Eleutherococcus senticosus on survival of cultured mammalian cells after ionizing radiation", pp.48~56 (1981)
- Boyle, W.J, "Osteoclast differentiation and activation", 423, pp.337~342 (2003)
- McCarthy, "Growth factors and the regulation of bone remodeling", pp.277~281 (1988)
- Res, "Effect of growth factors on bone cell replication and differentiation", pp.246~263 (1985)
- "Pharmacology and applications of Chinese amteria medica edited by Yao", 1, pp.~461 (1986)
- "Hot water-extracted Lycium barbarum and Rehmannia glutinosa inhibit proliferation and induce apoptosis of hepatocellular carcinoma cells", 12, pp.4478~4484 (2006)
- "Bisphosphonates: Mechanism of action and clinical use", pp.1037~1052 (1996), Academic Press
- "Protective effects of Acanthopanax senticosus Harms from Hokkaido and its components on gastric ulcer inrestrained cold water stressed rats", 19, pp.1227~1230 (1996)
- "Calcium. in Principles of Bone biology", pp.1007~1017 (1996), Academic Press
- "Herbal formulation, Yukmi-jihang-tang-Jahage, regulates bone resorption by inhibition of phosphorylation mediated by tyrosine kinase Src and cyclooxygenase expression", 106, pp.333~343 (2006)
- "Antioxidant property of an active component purified from the leaves of paraquat-tolerant Rehmannia glutinosa", 10, pp.311~318 (2005)
- "Effect of a combination of extract from several plants on cellmediated and humoral immunity of patients with advanced ovarian cancer", 20, pp.424~425 (2006)
- "A physiological and pharmacological inhibitor of bone resorption", 7, pp.613~635 (2001)
- "Mitochondrial granules in chondrocytes", pp.273~278 (1970), An ultrastructural and microincineration study
- "Tartrate-resistant acid phosphatase as a marker of osteoclast function", pp.285~290 (1982)
- "edited by Peck", pp.209~279 (1987)
- "Postmenopausal hormone replacement therapy: scientific review", 288, pp.872~881 (2002)
- "Cells of bone", pp.855~886 (1986)
- "Lineage of osteogenic cells and their relationship to the stromal system edited by Peck", pp.1~25 (1985), Elsevier Science Publisher
- "CSF-1 regulation of the wandering macrophage Complexity in action", 14, pp.628~638 (2004)
- "Hormonal regulation of bone growth and remodeling in Cell and Molecular Biology of Vertebrate Hard Tissue CIBA Foundation Symposium 136", pp.226~238 (1988)
- "Antioxidant iridoid glucosides from Wendlandia formosana", 8, pp.357~364 (2004)
- "Current therapy for osteoporosis", endocri(l), pp.14~16 (1993)
- "Treatment of bone in elderly subjects: calcium, vitamin D, fluor, bisphosphonates, calcitonin", 43, pp.83~88 (1995)
- "Emerging therapies in osteoporosis", pp.275~285 (1994)
- "Multinucleated cells formed on calcified dentin from mouse bone marrow cells treated with 1a", pp.379~391 (1989)
- "In vitro osteoclast generation from different bone marrow fractions", pp.79~321 81 (1986), including a highly enriched hematopoietic stem cell population
- "Suppressive effect of Chinese medicinal herb extract on human lympocytes in vitro", 118, pp.41~48 (1999)
- "Immunopharmacological effects of olysaccharides from Acanthopanax senticosus on experimental animals", immu(pharmacol), pp.549~554 (1991)
- "New acylated iridoid glucosides from Vitex altissima", 67, pp.2012~2016 (2004)
- "Drug therapy", pp.173~187 (1996), Humana Press, Totowa, NJ
- "Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families", 20, pp.345~357 (1999)
- "Induction of calcitonin receptors by 1a 25-dihydroxyvitamin D3 in osteoclast-like multinucleated cells formed from mouse bone marrow cells", endocri(l), pp.1504~1510 (1988)
- "Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures", endocri(l), pp.1373~1382 (1988)
- Ida, "Effect of Eleutherococcus senticosus and its rectal temperature and exploratory and spontaneous movements in acute stressed mice", pp.232~237 (1985)
- Tang, "Chinese Drugs of Plant Origin and Use in Traditional Modern Medicine", pp.849~854, Springer-Verlag
- Wang, "Immunomodulatory activity of polysaccharide from Acanthopanax obovatus roots", pp.335~336 (1991)
- "Antitumor activity of polysaccharide form a Chinese medicinal herb", pp.79~84 (1992)
- "What is an osteoporotic fracture edited by Rosen", pp.79~88 (1996), Diagnotic and Therapeutic Principles Humana Press
- "Protective effect of Rehmannia glutinosa on the cisplatin-induced damage of HEI-OC1 auditory cells through scavenging free radicals", eth(pharmacol), pp.383~388 (2006)
- "Extraction and salting-out purification of alpha-galactosidase and betaglucosidase from fresh roots of Rehmannia glutinosa", pp.137~139 (2006)
