우슬과 인삼 열수추출 혼합물의 파골세포와 조골세포 분화 효과
Effects of the Hot Water Extract Mixtures from Achyranthes bidentata Blume and Panax ginseng on Osteoclast and Osteoblast Differentiation
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Osteoporosis induces a bone mineral density loss due to imbalance of bone homeostasis that is achieved by osteoclasts (which are involved in bone resorption) and osteoblasts (which are involved in bone formation). Thus, this study was performed to evaluate the effects of hot water extract of the Achyranthes bidentata Blume (ABB) and Panax ginseng (Gin) on osteoclast and osteoblast differentiation. In this study, there was no cytotoxicity by ABB, 50 and 100 μg/ml of Gin significantly decreased cell viability of RANKL-induced osteoclast in RAW264.7 cell (p < 0.01). But, it was 50 μg/ml of ABB and Gin mixtures increased due to protective action of ABB. Furthermore, Gin contained groups (Gin, ABB and Gin mixtures) were inhibitory effects on osteoclast differentiation and bone resorption, and increased in osteoblast differentiation activity. Gin clearly inhibited RANKL-induced osteoclast differentiation by decreased calcitonin and TRAP (p < 0.01). Also, these extracts significantly increased calcium accumulation formation of osteoblastic differentiation reagents-induced osteoblast in MC3T3-E1 cell (p < 0.05). These results suggest that ABB and Gin mixtures may be a potential as drug for the treatment of osteoporosis.
Abstract
Osteoporosis induces a bone mineral density loss due to imbalance of bone homeostasis that is achieved by osteoclasts (which are involved in bone resorption) and osteoblasts (which are involved in bone formation). Thus, this study was performed to evaluate the effects of hot water extract of the Achyranthes bidentata Blume (ABB) and Panax ginseng (Gin) on osteoclast and osteoblast differentiation. In this study, there was no cytotoxicity by ABB, 50 and 100 μg/ml of Gin significantly decreased cell viability of RANKL-induced osteoclast in RAW264.7 cell (p < 0.01). But, it was 50 μg/ml of ABB and Gin mixtures increased due to protective action of ABB. Furthermore, Gin contained groups (Gin, ABB and Gin mixtures) were inhibitory effects on osteoclast differentiation and bone resorption, and increased in osteoblast differentiation activity. Gin clearly inhibited RANKL-induced osteoclast differentiation by decreased calcitonin and TRAP (p < 0.01). Also, these extracts significantly increased calcium accumulation formation of osteoblastic differentiation reagents-induced osteoblast in MC3T3-E1 cell (p < 0.05). These results suggest that ABB and Gin mixtures may be a potential as drug for the treatment of osteoporosis.
참고문헌 (35)
- Aldercreutz H, "Phyto-estrogens in relation to cancer and other human health risks", Proceedings of the Nutrition, 55, pp.399~417 (1996)
- Boyle WJ, "Osteoclast differentiation and activation", Nature, 423, pp.337~342 (2003)
- Chen XM, "Structural elucidation and antitumor activity of a fructan from Cyathula officinalis Kuan", Carbohydrate Research, 338, pp.1235~1241 (2003)
- Cheng B, "Ginsenoside Rb1 inhibits osteoclastogenesis by modulating NFkB and MAPKs pathways", Food and Chemical Toxicology, 50, pp.1610~1615 (2012)
- 최선영, "방사선조사가 MC3T3-E1 조골세포주의 type I collagen과alkaline phosphatase mRNA 발현에 미치는 영향", Imaging Science in Dentistry, 33(1), pp.51~57 (2003), 대한구강악안면방사선학회
- 최운용, "증숙 발효 공정에 의한 파삼의 진세노사이드 전환 수율 증진", 한국약용작물학회지, 22(3), pp.223~230 (2014), 한국약용작물학회[DOI]
- 최유란, "배양 골모세포에 있어서 골다공증의 유발물질인 카드뮴에 대한 우슬 추출물의 보호 효과", 한국인간식물환경학회지, 15(5), pp.319~324 (2012), 한국인간식물환경학회
- Darnay BG, "Activation of NF-kappa B by RANK requires tumor necrosis factor receptor-associated factor(TRAF)6 and NF-kappa B inducing kinase. Identification of a novel TRAF6 interaction motif", Journal of Biological Chemistry, 274, pp.7724~7731 (1999)
- Del FA, "Genetics, pathogenesis and complications of osteopetrosis", Bone, 42, pp.19~29 (2008)
- Grigoriades AE, "c-Fos : A key regulator of osteoclast-macrophage lineage determination and bone remodeling", Science, 266, pp.443~448 (1994)
- 한상배, "Prevention of Arthritic Inflammation Using an Oriental Herbal Combination BDX-1 Isolated from Achyranthes bidentata and Atractylodes japonica", Archives of Pharmacal Research, 28(8), pp.902~908 (2005), 대한약학회
- He L, "Ginsenoside Rh2 inhibits osteoclastogenesis through downregulation of NF-B, NFATc1 and c-Fos", Bone, 50, pp.1207~1213 (2012)
- 정호경, "LPS로 유도한 RAW 264.7 세포의 염증반응에서 뚝새풀 에탄올추출물의 억제 효과", 한국약용작물학회지, 22(2), pp.98~104 (2014), 한국약용작물학회[DOI]
- Jung NP, "Cytotoxicity of white and red ginseng against cancer cells and their effects on the cell cycle", Journal of Ginseng Research, 24, pp.183~187 (2000)
- Kannel KA, "Adverse effects of bisphosphonates : Implications for osteoporosis management", Mayo Clinic Proceedings, 84, pp.632~638 (2009)
- Karst M, "Roles of stromal cell RANKL, OPG, and M-CSF expression in biphasic TGF-regulation of osteoclast differentiation", Journal of Cellular Physiology, 200, pp.99~106 (2004)
- Kim DY, "Ginsenoside Rd stimulates the differentiation and mineralization of osteoblastic MC3T3-E1 cells by activating AMP-activated protein kinase via the BMP-2 signaling pathway", Fitoterapia, 83, pp.215~222 (2012)
- 김은정, "골다공증 쥐에서 우슬의 농도별 투여에 따른 골대사의 생화학적 마커에 미치는 영향", 동의생리병리학회지, 21(4), pp.967~972 (2007), 한의병리학회
- 김은정, "우슬이 산화적 스트레스와 관련한 세포내 신호전달계에 미치는 영향", 동의생리병리학회지, 22(4), pp.841~848 (2008), 한의병리학회
- 김주호, "우슬의 파골세포 분화 억제와 골 흡수 억제효과", 대한본초학회지, 25(1), pp.65~74 (2010), 대한본초학회
- Kim SI, "10-Acetyl panaxitriol, a new cytotoxic polyacetylene from Panax ginseng", Yakhak Hoeji, 33, pp.118~123 (1989)
- Kim Y, "Contribution of NFATc1 to the transcriptional control of immunoreceptor OSCAR but not TREM-2 during osteoclastogenesis", Journal of Biological Chemistry, 280, pp.32905~32913 (2005)
- Komori T., "Regulation of osteoblast differentiation by transcription factors", Journal of Cell Biochemistry, 99, pp.1233~1239 (2006)
- 곽한복, "녹용 물 추출물의 파골세포 분화 억제효과", 동의생리병리학회지, 22(4), pp.891~895 (2008), 한의병리학회
- Lee HY, "Aqueous ginseng extract has a preventive role in RANKL-induced osteoclast differentiation and estrogen deficiency-induced osteoporosis", Journal of Functional Foods, 13, pp.192~203 (2015)
- 이준원, "생약의 조골 세포 증식과 분화 검색", 생약학회지, 40(3), pp.190~195 (2009), 한국생약학회
- Li J, "Simultaneous determination of main phytoecdysones and triterpenoids in radix Achyranthis bidentatae by high-performance liquid chromatography with diode array-evaporative light scattering detectors and mass spectrometry", Analytica Chimica Acta, 596, pp.264~272 (2007)
- Matsumoto M, "Essential role of p38 mitogen-activated protein kinase in cathepsin K gene expression during osteoclastogenesis through association of NFATc1and PU. 1", Journal of Biological Chemistry, 279, pp.45969~45979 (2004)
- Mok SK, "The effects of prostagladine and dibutyryl cAMP on osteoblastic cell activity and osteoclast generation", Journal of Wonkwang Dental Research International, 6, pp.43~62 (1996)
- Pole HA, "Multinational, placebo-controlled, randomized trial of the effects of alendronate on bone density and fracture risk in postmenopausal women with low bone mass : Results of the FOSIT study", Osteoporosis International, 9, pp.461~468 (1999)
- Takayanagi H, "Induction and activation of the transcription factor NFATc1(NFAT2)integrate RANKL signaling in terminal differentiation of osteoclasts", Developmental Cell, 3, pp.889~901 (2002)
- Tintut Y, "8-Isoprostaglandin E2 enhances receptor-activated NFkappa B ligand(RANKL)-dependent osteoclastic potential of marrow hematopoietic precursors via the cAMP pathway", Journal of Biological Chemistry, 277, pp.14221~14226 (2002)
- Tsai HY, "Paeonol inhibits RANKL-induced osteoclastogenesis by inhibiting ERK, p38 and NF-B pathway", European Journal of Pharmacology, 588, pp.124~133 (2008)
- Yavropoulou MP, "Osteoclstogenesis current knowledge and future perspectives", Journal of Musculoskeletal and Neuronal Interactions, 8, pp.204~216 (2008)
- 윤강휴, "대추 물 추출물이 RANKL에 의해 유도되는 파골세포 분화에 미치는 영향", 동의생리병리학회지, 28(1), pp.29~34 (2014), 한의병리학회