Binding of Tp92 homolog of Treponemadenticolatofibronectinandepithelialcells
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Treponema denticola is the best studied oral spirochete and numerous studies have shown that it is strongly associated with periodontitis and expresses several putative virulence factors. In this study, we report on a surface protein of T. denticola, Td92, which is homologous to Tp92 of Treponema pallidum, an agent of syphilis. Immunofluorescence assay and immunogold labeling with anti- Td92 Ab revealed that Td92 had surface-exposed epitopes. And Td92 was capable of binding to fibronectin and KB cells, an oral epithelial cell line. In addition, Td92 could enter the KB cells. These results indicate that Td92 is a fibronectin-binding protein which can bind to and internalize into the host cells, facilitating the virulence of T. denticola.
Abstract
Treponema denticola is the best studied oral spirochete and numerous studies have shown that it is strongly associated with periodontitis and expresses several putative virulence factors. In this study, we report on a surface protein of T. denticola, Td92, which is homologous to Tp92 of Treponema pallidum, an agent of syphilis. Immunofluorescence assay and immunogold labeling with anti- Td92 Ab revealed that Td92 had surface-exposed epitopes. And Td92 was capable of binding to fibronectin and KB cells, an oral epithelial cell line. In addition, Td92 could enter the KB cells. These results indicate that Td92 is a fibronectin-binding protein which can bind to and internalize into the host cells, facilitating the virulence of T. denticola.
참고문헌 (20)
- Batista Da Silva, A. P., "The major outer sheath protein of Treponema denticola inhibits the binding step of collagen phagocytosis in fibroblasts", Cellular Microbiology, 6, pp.485~498 (2004)
- Cameron C. E., "Opsonic potential, protective capacity, and sequence conservation of the Treponema pallidum subspecies pallidum Tp9", Journal of Infectious Diseases, 181, pp.1401~1413 (2000)
- Chi, B., "Role of dentilisin in Treponema denticola epithelial cell layer penetration", Research in Microbiology, 154, pp.637~643 (2003)
- Chia, J. S., "Identification of a fibronectin binding protein from Streptococcus mutans", Infection and Immunity, 통권 : 68, pp.1864~1870 (2000)
- Edwards, A. M., "Binding properties and adhesion-mediating regions of the major sheath protein of Treponema denticola ATCC 35405", Infection and Immunity, 73, pp.2891~2898 (2005)
- Ellen, R. P, "Spirochetes at the forefront of periodontal infections", Periodontology 2000, 38, pp.13~32 (2005)
- Fenno, J. C., "Cytopathic effects of the major surface protein and the chymotrypsinlike protease of Treponema denticola", Infection and Immunity, 66, pp.1869~1877 (1998)
- Fenno, J. C., "Sequence analysis, expression, and binding activity of recombinant major outer sheath protein (Msp) of Treponema denticola", Journal of Bacteriology, 178, pp.2489~2497 (1996)
- Fenno, J. C., "Identification of a Treponema denticola OppA homologue that binds host proteins present in the subgingival environment", Infection and Immunity, 68, pp.1884~1892 (2000)
- Fowler, T., "Cellular invasion by Staphylococcus aureus involves a fibronectin bridge between the bacterial fibronectin-binding MSCRAMMs and host cell beta1 integrins", European Journal of Cell Biology, 79, pp.672~679 (2000)
- Hall-Stoodley, L., "Mycobacterium tuberculosis binding to human surfactant proteins A and D, fibronectin, and small airway epithelial cells under shear conditions", Infection and Immunity, 74, pp.3587~3596 (2006)
- Innes, N. P, "A technique for the study of endocytosis in human oral epithelial cells", Archives of Oral Biology, 44, pp.519~523 (1999)
- Lee, S. H., "Upregulation of intercellular adhesion molecule 1 and proinflammatory cytokines by the major surface proteins of Treponema maltophilum and Treponema lecithinolyticum, the phylogenetic group IV oral spirochetes associated with periodontitis and endodontic infections", Infection and Immunity, 73, pp.268~276 (2005)
- Mathers, D. A., "The major surface protein complex of Treponema denticola depolarizes and induces ion channels in HeLa cell membranes. Infect", Infection and Immunity, 64, pp.2904~2910 (1996)
- Miyamoto, M., "The Treponema denticola surface protease dentilisin degrades interleukin-1 beta (IL-1 beta), IL-6, and tumor necrosis factor alpha", Infection and Immunity, 74, pp.2462~2467 (2006)
- Van Voorhis, W. C., "Serodiagnosis of syphilis: antibodies to recombinant Tp0453, Tp92, and Gpd proteins are sensitive and specific indicators of infection by Treponema pallidum", Journal of Clinical Microbiology, 41, pp.3668~3674 (2003)
- Yang, P. F., "Filamentous actin disruption and diminished inositol phosphate response in gingival fibroblasts caused by Treponema denticola", Infection and Immunity, 66, pp.696~702 (1998)
- Walker, S. G., "Identification, isolation, and characterization of the 42-kilodalton major outer membrane protein (MompA) from Treponema pectinovorum ATCC 33768", Journal of Bacteriology, 179, pp.6441~6447 (1997)
- Wang, Q., "A spirochete surface protein uncouples store-operated calcium channels in fibroblasts: a novel cytotoxic mechanism", Journal of Biological Chemistry, 276, pp.23056~23064 (2001)
- Wyss, C., "Treponema lecithinolyticum sp. nov., a small saccharolytic spirochaete with phospholipase A and C activities associated with periodontal diseases", International Journal of Systematic Bacteriology, 49, pp.1329~1339 (1999)