Surface interactions between two of the main periodontal pathogens: Porphyromonas gingivalis and Tannerella forsythia
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purpose: Porphyromonas gingivalis and Tannerella forsythia have been implicated as the major etiologic agents of periodontal disease. These two bacteria are frequently isolated together from the periodontal lesion, and it has been suggested that their interaction may increase each one’s virulence potential. The purpose of this study was to identify proteins on the surface of these organisms that are involved in interbacterial binding. Methods: Biotin labeling of surface proteins of P. gingivalis and T. forsythia and liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis was performed to identify surface proteins involved in the coaggregating activity between P. gingivalis and T. forsythia. Results: It was found that three major T. forsythia proteins sized 161, 100, and 62 kDa were involved in binding to P. gingivalis , and P. gingivalis proteins sized 35, 32, and 26 kDa were involved in binding to T. forsythia cells. Conclusions: LC-MS/MS analysis identified one T. forsythia surface protein (TonB-linked outer membrane protein) involved in interbacterial binding to P. gingivalis. However, the nature of other T. forsythia and P. gingivalis surface proteins identified by biotin labeling could not be determined. Further analysis of these proteins will help elucidate the molecular mechanisms that mediate coaggregation between P. gingivalis and T. forsythia.
Abstract
Purpose: Porphyromonas gingivalis and Tannerella forsythia have been implicated as the major etiologic agents of periodontal disease. These two bacteria are frequently isolated together from the periodontal lesion, and it has been suggested that their interaction may increase each one’s virulence potential. The purpose of this study was to identify proteins on the surface of these organisms that are involved in interbacterial binding. Methods: Biotin labeling of surface proteins of P. gingivalis and T. forsythia and liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis was performed to identify surface proteins involved in the coaggregating activity between P. gingivalis and T. forsythia. Results: It was found that three major T. forsythia proteins sized 161, 100, and 62 kDa were involved in binding to P. gingivalis , and P. gingivalis proteins sized 35, 32, and 26 kDa were involved in binding to T. forsythia cells. Conclusions: LC-MS/MS analysis identified one T. forsythia surface protein (TonB-linked outer membrane protein) involved in interbacterial binding to P. gingivalis. However, the nature of other T. forsythia and P. gingivalis surface proteins identified by biotin labeling could not be determined. Further analysis of these proteins will help elucidate the molecular mechanisms that mediate coaggregation between P. gingivalis and T. forsythia.
참고문헌 (29)
- Finlay BB, "Common themes in microbial pathogenicity revisited", Microbiol Mol Biol Rev, 61, pp.136~169 (1997)
- Marsh PD, "Dental plaque : biological significance of a biofilm and community life-style", J Clin Periodontol, 32(Suppl 6), pp.7~15 (2005)
- Philip D. Marsh, "Dental plaque biofilms: communities, conflict and control", Periodontology 2000, 55(1), pp.16~35 (2011), Wiley-Blackwell[DOI]
- B. J. Paster, "Bacterial Diversity in Human Subgingival Plaque", Journal of Bacteriology, 183(12), pp.3770~3783 (2001), American Society for Microbiology[DOI]
- Sigmund S. Socransky, "Evidence of bacterial etiology: a historical perspective", Periodontology 2000, 5(1), pp.7~25 (1994), Wiley-Blackwell[DOI]
- I. Kroes, "Bacterial diversity within the human subgingival crevice", Proceedings of the National Academy of Sciences, 96(25), pp.14547~14552 (1999), Proceedings of the National Academy of Sciences[DOI]
- J. A. Aas, "Defining the Normal Bacterial Flora of the Oral Cavity", Journal of Clinical Microbiology, 43(11), pp.5721~5732 (2005), American Society for Microbiology[DOI]
- P. D. Marsh, "Dental plaque: biological significance of a biofilm and community life-style", Journal of Clinical Periodontology, 32(s6), pp.7~15 (2005), Wiley-Blackwell[DOI]
- S.S. Socransky, "Microbial complexes in subgingival plaque", Journal of Clinical Periodontology, 25(2), pp.134~144 (1998), Wiley-Blackwell[DOI]
- Haffajee AD, "Microbial etiological agents of destructive periodontal diseases", Periodontol 2000, 5, pp.78~111 (1994)
- Darveau RP, "Periodontitis: a polymicrobial disruption of host homeostasis", Nat Rev Microbiol, 8, pp.481~490 (2010)
- Kolenbrander PE, "Coaggregations among oral bacteria", Methods Enzymol, 253, pp.385~397 (1995)
- Kolenbrander PE, "Communication among oral bacteria", Microbiol Mol Biol Rev, 66, pp.486~505 (2002)
- Kirschbaum M, "Mixture of periodontopathogenic bacteria influences interaction with KB cells", Anaerobe, 16, pp.461~468 (2010)
- Kolenbrander PE, "Oral microbial communities: biofilms, interactions, and genetic systems", Annu Rev Microbiol, 54, pp.413~437 (2000)
- Kesavalu L, "Rat model of polymicrobial infection, immunity, and alveolar bone resorption in periodontal disease", Infect Immun, 75, pp.1704~1712 (2007)
- Zambon JJ, "Periodontal diseases: microbial factors", Ann Periodontol, 1, pp.879~925 (1996)
- Kamma JJ, "Predominant microflora of severe, moderate and minimal periodontal lesions in young adults with rapidly progressive periodontitis", J Periodontal Res, 30, pp.66~72 (1995)
- Yoneda M, "Mixed infection of Porphyromonas gingivalis and Bacteroides forsythus in a murine abscess model: involvement of gingipains in a synergistic effect", J Periodontal Res, 36, pp.237~243 (2001)
- Takemoto T, "Characterization of Bacteroides forsythus isolates", J Clin Microbiol, 35, pp.1378~1381 (1997)
- Yoneda M, "Stimulation of growth of Porphyromonas gingivalis by cell extracts from Tannerella forsythia", J Periodontal Res, 40, pp.105~109 (2005)
- Yao ES, "Interbacterial binding among strains of pathogenic and commensal oral bacterial species", Oral Microbiol Immunol, 11, pp.35~41 (1996)
- 엄흥식, "Porphyromonas gingivalis와 Tannerella forsythia의 응집반응", Journal of Periodontal & Implant Science, 36(1), pp.265~272 (2006), 대한치주과학회
- Potempa J, "Gingipains, the major cysteine proteinases and virulence factors of Porphyromonas gingivalis : structure, function and assembly of multidomain protein complexes", Curr Protein Pept Sci, 4, pp.397~407 (2003)
- Kamaguchi A, "Adhesins encoded by the gingipain genes of Porphyromonas gingivalis are responsible for co-aggregation with Prevotella intermedia", Microbiology, 149, pp.1257~1264 (2003)
- N. Shimotahira, "The Surface Layer of Tannerella forsythia Contributes to Serum Resistance and Oral Bacterial Coaggregation", Infection and Immunity, 81(4), pp.1198~1206 (2013), American Society for Microbiology[DOI]
- M. Sabet, "The surface (S-) layer is a virulence factor of Bacteroides forsythus", Microbiology, 149, pp.3617~3627 (2003)
- Lee SW, "Identification and characterization of the genes encoding a unique surface (S-) layer of Tannerella forsythia", Gene, 371, pp.102~111 (2006)
- Yoo JY, "Identification of Tannerella forsythia antigens specifically expressed in patients with periodontal disease", FEMS Microbiol Lett, 275, pp.344~352 (2007)