임상 분리 Trichophyton 균주에 대한 Luliconazole의 생체 외 감수성 검사
In vitro Antifungal Susceptibility Testing of Clinically-isolated Trichophyton Species to Luliconazole
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Background: Luliconazole (LLCZ) is an imidazole antifungal agent widely used in Japan for treatment of tinea, cutaneous candidiasis, and pityriaisis versicolor, etc. Theoretically, its potent antifungal activity should make it a viable option for various conditions of dermatophytoses, but there has been no studies to support the notion thus far. Objective: This prompted the authors to investigate the distribution of minimum inhibitory concentration (MIC) of luliconazole by carrying out in vitro antifungal susceptibility test on Trichophyton (T.) species clinically isolated from Korean subjects with tinea pedis. Methods: In vitro antifungal susceptibility test, with broth macrodilution method of luliconazole and terbinafine hydrochloride (TBF) as reference drug on two clinically isolated Trichophton species (22strains of T. rubrum, 5 of T. mentagrophytes), was carried out. Results: The range of MIC for LLCZ on the total of 27 strains was 0.0110~0.0140 μg/mL (geometric mean: 0.0027 μg/mL), and the range for TBF was 0.0120~0.0250 μg/mL (geometric mean: 0.0042μg/mL). Additionally, the geometric mean for MIC of LLCZ on T. mentagrophytes was 0.0125 μg/mL,and on T. rubrum, it was 0.0019 μg/mL. No strain showed resistance in vitro. Conclusions: The authors hence demonstrated that antifungal activity of LLCZ in vitro against Trichophyton species clinically isolated from Korean subjects is quite superior. These findings emphasize the usefulness of LLCZ, a promising new imidazole, for the topical management of dermatophytoses.
Abstract
Background: Luliconazole (LLCZ) is an imidazole antifungal agent widely used in Japan for treatment of tinea, cutaneous candidiasis, and pityriaisis versicolor, etc. Theoretically, its potent antifungal activity should make it a viable option for various conditions of dermatophytoses, but there has been no studies to support the notion thus far. Objective: This prompted the authors to investigate the distribution of minimum inhibitory concentration (MIC) of luliconazole by carrying out in vitro antifungal susceptibility test on Trichophyton (T.) species clinically isolated from Korean subjects with tinea pedis. Methods: In vitro antifungal susceptibility test, with broth macrodilution method of luliconazole and terbinafine hydrochloride (TBF) as reference drug on two clinically isolated Trichophton species (22strains of T. rubrum, 5 of T. mentagrophytes), was carried out. Results: The range of MIC for LLCZ on the total of 27 strains was 0.0110~0.0140 μg/mL (geometric mean: 0.0027 μg/mL), and the range for TBF was 0.0120~0.0250 μg/mL (geometric mean: 0.0042μg/mL). Additionally, the geometric mean for MIC of LLCZ on T. mentagrophytes was 0.0125 μg/mL,and on T. rubrum, it was 0.0019 μg/mL. No strain showed resistance in vitro. Conclusions: The authors hence demonstrated that antifungal activity of LLCZ in vitro against Trichophyton species clinically isolated from Korean subjects is quite superior. These findings emphasize the usefulness of LLCZ, a promising new imidazole, for the topical management of dermatophytoses.
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