Expression of Neurotrophic Factors and Their Receptors in Rat Posterior Taste Bud Cells
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Taste is an important sense in survival and growth ofanimals. The growth and maintenance of taste buds, thereceptor organs of taste sense, are under the regulation ofvarious neurotrophic factors. But the distribution aspect ofneurotrophic factors and their receptors in distinct taste celltypes are not clearly known. The present research wasdesigned to characterize mRNA expression pattern ofneurotrophic factors and their receptors in distinct type of tastecells. In male 45-60 day-old Sprague-Dawley rats, epithelialtissues with and without circumvallate and folliate papillaeswere dissected and homogenized, and mRNA expressions forneurotrophic factors and their receptors were determined byRT-PCR. The mRNA expressions of brain-derivedneurotrophic factor (BDNF), neurotrophin-3 (NT3), receptortyrosine kinase B (TrkB), exclusion of nerve growth factor(NGF), neurotrophin-4/5 (NT4/5), receptor tyrosine kinase A(TrkA), receptor tyrosine kinase C (TrkC), and p75NGFRwere observed in some population of taste cell. In support ofthis result and to characterize which types of taste cells expressNT3, BDNF, or TrkB, we examined mRNA expressions of NT3, BDNF, or TrkB in the PLCβ2 (a marker of Type II cell)-and/or SNAP25 (a marker of Type III cell)-positive taste cellsby a single taste cell RT-PCR and found that the ratio ofpositively stained cell numbers were 17.4, 6.5, 84.1, 70.3, and1.4 % for PLCβ2, SNAP25, NT3, BDNF, and TrkB,respectively. In addition, all of PLCβ2- and SNAP25-positivetaste cells expressed NT3 mRNA, except for one taste bud cell. The ratios of NT3 mRNA expressions were 100% and 91.7%in the SNAP25- and PLCβ2-positive taste cells, respectively. However, two TrkB-positive taste cells co-expressed neitherPLCβ2 nor SNAP 25. The results suggest that the most of typeII or type III cells express BDNF and NT3 mRNA, but theexpression is shown to be less in type I taste cells.
Abstract
Taste is an important sense in survival and growth ofanimals. The growth and maintenance of taste buds, thereceptor organs of taste sense, are under the regulation ofvarious neurotrophic factors. But the distribution aspect ofneurotrophic factors and their receptors in distinct taste celltypes are not clearly known. The present research wasdesigned to characterize mRNA expression pattern ofneurotrophic factors and their receptors in distinct type of tastecells. In male 45-60 day-old Sprague-Dawley rats, epithelialtissues with and without circumvallate and folliate papillaeswere dissected and homogenized, and mRNA expressions forneurotrophic factors and their receptors were determined byRT-PCR. The mRNA expressions of brain-derivedneurotrophic factor (BDNF), neurotrophin-3 (NT3), receptortyrosine kinase B (TrkB), exclusion of nerve growth factor(NGF), neurotrophin-4/5 (NT4/5), receptor tyrosine kinase A(TrkA), receptor tyrosine kinase C (TrkC), and p75NGFRwere observed in some population of taste cell. In support ofthis result and to characterize which types of taste cells expressNT3, BDNF, or TrkB, we examined mRNA expressions of NT3, BDNF, or TrkB in the PLCβ2 (a marker of Type II cell)-and/or SNAP25 (a marker of Type III cell)-positive taste cellsby a single taste cell RT-PCR and found that the ratio ofpositively stained cell numbers were 17.4, 6.5, 84.1, 70.3, and1.4 % for PLCβ2, SNAP25, NT3, BDNF, and TrkB,respectively. In addition, all of PLCβ2- and SNAP25-positivetaste cells expressed NT3 mRNA, except for one taste bud cell. The ratios of NT3 mRNA expressions were 100% and 91.7%in the SNAP25- and PLCβ2-positive taste cells, respectively. However, two TrkB-positive taste cells co-expressed neitherPLCβ2 nor SNAP 25. The results suggest that the most of typeII or type III cells express BDNF and NT3 mRNA, but theexpression is shown to be less in type I taste cells.
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