Anticancer Properties of Icariside II in Human Oral Squamous Cell Carcinoma Cells
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
OSCC is currently the most common malignancy of the head and neck, affecting tens of thousands of patients per year worldwide. Natural flavonoids from plants are potential sources for novel anti-cancer drugs. Icariin is the active ingredient of flavonol glycoside, which is derived from the medical plant Herba Epimedii. A metabolite of icariin, icariside II exhibits a variety of pharmacological actions, including anti-rheumatic, anti-depressant, cardiovascular protective, and immunomodulatory functions. However, the exact mechanism causing the apoptosis-inducing effect of icariside II in OSCC is still not fully understood. In the present study, we assessed the anti-cancer effect of icariside II in OSCC cell lines by measuring its effect on cell viability, cell proliferation, and mitochondria membrane potential (MMP). Icariside II treatment of OSCC cells resulted in a dose- and time-dependent decrease in cell viability. Hoechst staining indicated apoptosis in icariside II-treated HSC cells. Icariside II inhibited cell proliferation and induced apoptosis in HSC cells, with significant increases in all present parameters in HSC-4 cells. The results clearly suggested that icariside II induced apoptosis via activation of intrinsic pathways and caspase cascades in HSC-4 cell lines. The collective findings of the study suggested that Icariside II is a potential treatment for OSCC; in addition, the data could provide a basis for the development of a novel anti-cancer strategy.
Abstract
OSCC is currently the most common malignancy of the head and neck, affecting tens of thousands of patients per year worldwide. Natural flavonoids from plants are potential sources for novel anti-cancer drugs. Icariin is the active ingredient of flavonol glycoside, which is derived from the medical plant Herba Epimedii. A metabolite of icariin, icariside II exhibits a variety of pharmacological actions, including anti-rheumatic, anti-depressant, cardiovascular protective, and immunomodulatory functions. However, the exact mechanism causing the apoptosis-inducing effect of icariside II in OSCC is still not fully understood. In the present study, we assessed the anti-cancer effect of icariside II in OSCC cell lines by measuring its effect on cell viability, cell proliferation, and mitochondria membrane potential (MMP). Icariside II treatment of OSCC cells resulted in a dose- and time-dependent decrease in cell viability. Hoechst staining indicated apoptosis in icariside II-treated HSC cells. Icariside II inhibited cell proliferation and induced apoptosis in HSC cells, with significant increases in all present parameters in HSC-4 cells. The results clearly suggested that icariside II induced apoptosis via activation of intrinsic pathways and caspase cascades in HSC-4 cell lines. The collective findings of the study suggested that Icariside II is a potential treatment for OSCC; in addition, the data could provide a basis for the development of a novel anti-cancer strategy.
참고문헌 (35)
- Clayman GL, "Report of the Tenth International Symposium of the Foundation for Promotion of Cancer Research: Basic and clinical research in head and neck cancer", Japanese Journal of Clinical Oncology, 27, pp.361~368 (1997)[DOI]
- Casiglia J, "A comprehensive review of oral cancer", General dentistry, 49, pp.72~82 (2000)
- R. Bryan Bell, "Tongue Cancer: Is There a Difference in Survival Compared With Other Subsites in the Oral Cavity?", Journal of Oral and Maxillofacial Surgery, 65(2), pp.229~236 (2007), Elsevier BV[DOI]
- Wen-Liang Lo, "Outcomes of oral squamous cell carcinoma in Taiwan after surgical therapy: factors affecting survival", Journal of Oral and Maxillofacial Surgery, 61(7), pp.751~758 (2003), Elsevier BV[DOI]
- Satoru Shintani, "Anti-tumor effect of radiation response by combined treatment with angiogenesis inhibitor, TNP-470, in oral squamous cell carcinoma", Oral Oncology, 42(1), pp.65~71 (2006), Elsevier BV[DOI]
- Shilpa Sarvothaman, "Apoptosis: role in myeloid cell development", Blood Research, 50(2), pp.73~79 (2015), 대한혈액학회
- I. Tedesco, "Dealcoholated red wine induces autophagic and apoptotic cell death in an osteosarcoma cell line", Food and Chemical Toxicology, 60, pp.377~384 (2013), Elsevier BV[DOI]
- N. Hail, "Apoptosis effector mechanisms: A requiem performed in different keys", Apoptosis, 11(6), pp.889~904 (2006), Springer Nature[DOI]
- Hongrui Guo, "Modulation of the PI3K", Akt Pathway and Bcl-2 Family Proteins Involved in Chicken’s Tubular Apoptosis Induced by Nickel Chloride (NiCl2), 2015, pp.16(9), 22989~23011[DOI]
- Ahn G, "A sulfated polysaccharide of Ecklonia cava inhibits the growth of colon cancer cells by inducing apoptosis", EXCLI J, 14, pp.294~306 (2015)[DOI]
- Wen-jing Ruan, "Anticancer effects of Chinese herbal medicine, science or myth?", Journal of Zhejiang University SCIENCE B, 7(12), pp.1006~1014 (2006), Zhejiang University Press[DOI]
- Hertog MG, "Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study", The Lancet, 36(93), pp.92876~ (1993)[DOI]
- Kumar S, "Chemistry and biological activities of flavonoids: an overview", The Scientific World Journal, 2013(1) (2013)[DOI]
- Ying Pan, "Icariin from Epimedium brevicornum attenuates chronic mild stress-induced behavioral and neuroendocrinological alterations in male Wistar rats", Pharmacology Biochemistry and Behavior, 87(1), pp.130~140 (2007), Elsevier BV[DOI]
- Jian Liu, "Determination of icariin and metabolites in rat serum by capillary zone electrophoresis: rat pharmacokinetic studies after administration of icariin", Journal of Pharmaceutical and Biomedical Analysis, 36(2), pp.365~370 (2004), Elsevier BV[DOI]
- Quan Xia, "Preparation of icariside II from icariin by enzymatic hydrolysis method", Fitoterapia, 81(5), pp.437~442 (2010), Elsevier BV[DOI]
- Hai-Bin Xu, "Icariin enhances endothelial nitric-oxide synthase expression on human endothelial cells in vitro", Vascular Pharmacology, 47(1), pp.18~24 (2007), Elsevier BV[DOI]
- Chaoqing HUANG, "Induction of Apoptosis by Icariside II through Extrinsic and Intrinsic Signaling Pathways in Human Breast Cancer MCF7 Cells", Bioscience, Biotechnology, and Biochemistry, 76(7), pp.1322~1328 (2014), Informa UK Limited[DOI]
- Dian-cai Zhang, "Icariin potentiates the antitumor activity of gemcitabine in gallbladder cancer by suppressing NF-κB", Acta Pharmacologica Sinica, 34(2), pp.301~308 (2012), Springer Nature[DOI]
- Jing-Shan Tong, "Icaritin Causes Sustained ERK1", 2 Activation and Induces Apoptosis in Human Endometrial Cancer Cells, 2011, pp.6(3), e16781~[DOI]
- Diane F. Birt, "Dietary agents in cancer prevention: flavonoids and isoflavonoids", Pharmacology & Therapeutics, 90(2-3), pp.157~177 (2001), Elsevier BV[DOI]
- Sun-Hee Kim, "Janus activated kinase 2", signal transducer and activator of transcription 3 pathway mediates icariside II-induced apoptosis in U266 multiple myeloma cells, 2011, pp.654(1), 10~16[DOI]
- Jia-Yo Wu, "Potential biomarkers in saliva for oral squamous cell carcinoma", Oral Oncology, 46(4), pp.226~231 (2010), Elsevier BV[DOI]
- Manuel Oropesa Ávila, "Emerging roles of apoptotic microtubules during the execution phase of apoptosis", Cytoskeleton, 72(9), pp.435~446 (2015), Wiley-Blackwell[DOI]
- K. Nishida, "Crosstalk Between Autophagy and Apoptosis in Heart Disease", Circulation Research, 103(4), pp.343~351 (2008), Ovid Technologies (Wolters Kluwer Health)[DOI]
- Sun-Hee Kim, "Janus activated kinase 2", signal transducer and activator of transcription 3 pathway mediates icariside II-induced apoptosis in U266 multiple myeloma cells, 2011, pp.654(1), 10~16[DOI]
- Keun-Sung Lee, "Cyclooxygenase-2", prostaglandin E2 pathway mediates icariside II induced apoptosis in human PC-3 prostate cancer cells, 2009, pp.280(1), 93~100[DOI]
- Harada H, "Apoptosis regulators", Reviews in clinical and experimental hematology, 7, pp.117~138 (2003)
- H. Wajant, "The Fas Signaling Pathway: More Than a Paradigm", Science, 296(5573), pp.1635~1636 (2002), American Association for the Advancement of Science (AAAS)[DOI]
- L. Ouyang, "Programmed cell death pathways in cancer: a review of apoptosis, autophagy and programmed necrosis", Cell Proliferation, 45(6), pp.487~498 (2012), Wiley-Blackwell[DOI]
- I. M. Ghobrial, "Targeting Apoptosis Pathways in Cancer Therapy", CA: A Cancer Journal for Clinicians, 55(3), pp.178~194 (2005), Wiley-Blackwell[DOI]
- Daniel M. Wall, "Bacterial secreted effectors and caspase-3 interactions", Cellular Microbiology, 16(12), pp.1746~1756 (2014), Wiley-Blackwell[DOI]
- Julio Morales, "Review of Poly (ADP-ribose) Polymerase (PARP) Mechanisms of Action and Rationale for Targeting in Cancer and Other Diseases", Critical Reviews in Eukaryotic Gene Expression, 24(1), pp.15~28 (2014), Begell House[DOI]
- Susan Elmore, "Apoptosis: A Review of Programmed Cell Death", Toxicologic Pathology, 35(4), pp.495~516 (2007), SAGE Publications[DOI]
- S Nagata, "Degradation of chromosomal DNA during apoptosis", Cell Death and Differentiation, 10(1), pp.108~116 (2003), Springer Nature[DOI]
