Trifolium pratense induces apoptosis through caspase pathway in FaDu human hypopharynx squamous carcinoma cells
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Trifolium pratense leaves (red clover) has been used in Oriental and European folk medicine for the treatment of whooping cough, asthma, and eczema, and is now being used to treat and alleviate the symptoms, such as hot flushes, cardiovascular health effects that occur in postmenopausal women. However, relatively little scientific data is available on the physiological activity of this plant. Therefore, in this study, we investigated the anti-cancer activity of T. pratense leaves using methanol extract of T. pratense leaves (MeTP) on human FaDu hypopharyngeal squamous carcinoma cells. MeTP inhibited the viability of FaDu cells by inducing apoptosis through the cleavage of pro- caspase-3, -7, and -9 and poly (adenosine diphosphate ribose-ribose) polymerase (PARP), downregulation of Bcl- 2, and upregulation of Bax, as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Live & dead assay, 4’6-diamidino-2-phenylindole stain, fluorescence-activated cell sorting analysis, and Western blot analysis. In addition, colony formation was slightly inhibited when FaDu cells were treated with a non-cytotoxic concentration (0.125 mg/mL) of MeTP and almost completely inhibited when cells were treated with 0.25 mg/mL MeTP. Collectively, these results indicate that MeTP induced cell apoptosis via caspase- and mitochondrial-dependent apoptotic pathways, and inhibited colony formation of cancer cells in FaDu human hypopharyngeal squamous carcinoma cells. These findings suggest MeTP should be considered for clinical development as a chemotherapeutic option in oral cancer.
Abstract
Trifolium pratense leaves (red clover) has been used in Oriental and European folk medicine for the treatment of whooping cough, asthma, and eczema, and is now being used to treat and alleviate the symptoms, such as hot flushes, cardiovascular health effects that occur in postmenopausal women. However, relatively little scientific data is available on the physiological activity of this plant. Therefore, in this study, we investigated the anti-cancer activity of T. pratense leaves using methanol extract of T. pratense leaves (MeTP) on human FaDu hypopharyngeal squamous carcinoma cells. MeTP inhibited the viability of FaDu cells by inducing apoptosis through the cleavage of pro- caspase-3, -7, and -9 and poly (adenosine diphosphate ribose-ribose) polymerase (PARP), downregulation of Bcl- 2, and upregulation of Bax, as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Live & dead assay, 4’6-diamidino-2-phenylindole stain, fluorescence-activated cell sorting analysis, and Western blot analysis. In addition, colony formation was slightly inhibited when FaDu cells were treated with a non-cytotoxic concentration (0.125 mg/mL) of MeTP and almost completely inhibited when cells were treated with 0.25 mg/mL MeTP. Collectively, these results indicate that MeTP induced cell apoptosis via caspase- and mitochondrial-dependent apoptotic pathways, and inhibited colony formation of cancer cells in FaDu human hypopharyngeal squamous carcinoma cells. These findings suggest MeTP should be considered for clinical development as a chemotherapeutic option in oral cancer.
참고문헌 (9)
- J F R Kerr, "Apoptosis: A Basic Biological Phenomenon with Wideranging Implications in Tissue Kinetics", British Journal of Cancer, 26(4), pp.239~257 (1972), Springer Science and Business Media LLC[DOI]
- John F.R Kerr, "History of the events leading to the formulation of the apoptosis concept", Toxicology, 181-182, pp.471~474 (2002), Elsevier BV[DOI]
- Susan Elmore, "Apoptosis: A Review of Programmed Cell Death", Toxicologic Pathology, 35(4), pp.495~516 (2016), SAGE Publications[DOI]
- Chris J Norbury, "Cellular responses to DNA damage", Annual Review of Pharmacology and Toxicology, 41(1), pp.367~401 (2001), Annual Reviews[DOI]
- K. L. King, "CELL CYCLE REGULATION AND APOPTOSIS", Annual Review of Physiology, 60(1), pp.601~617 (1998), Annual Reviews[DOI]
- John F. R. Kerr, "Apoptosis. Its significance in cancer and cancer Therapy", Cancer, 73(8), pp.2013~2026 (1994), Wiley[DOI]
- Smets LA, "Programmed cell death(apoptosis)and response to anti-cancer drugs", Anticancer Drugs, 5, pp.3~9 (1994)
- Ashis Mukherjee, "Advances in Cancer Therapy with Plant Based Natural Products", Current Medicinal Chemistry, 8(12), pp.1467~1486 (2001), Bentham Science Publishers Ltd.[DOI]
- Leonidas Christou, "Treatment of plasma cell leukemia with vincristine, liposomal doxorubicin and dexamethasone", European Journal of Haematology, 67(1), pp.51~53 (2001), Wiley[DOI]