Inhibition of lyosphosphatidic acid receptor 1 signaling in periodontal ligament stem cells reduces inflammatory paracrine effect in primary astrocyte cells
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Lysophosphatidic acid (LPA) is a bioactive lipid messenger involved in the pathogenesis of chronic inflammation and various diseases. Recent studies have shown an association between periodontitis and neuroinflammatory diseases such as Alzheimer’s disease, stroke, and multiple sclerosis. However, the mechanistic relationship between periodontitis and neuroinflammatory diseases remains unclear. The current study found that lysophosphatidic acid receptors 1 (LPAR1) and 6 (LPAR6) exhibited increased expression in primary microglia and astrocytes. The primary astrocytes were then treated using medium conditioned to mimic periodontitis through addition of Porphyromonas gingivalis lipopolysaccharides, and an increased nitric oxide (NO) production was observed. Application of conditioned medium from human periodontal ligament stem cells with or without LPAR1 knockdown showed a decrease in the production of NO and expression of inducible nitric oxide synthase and interleukin 1 beta. These findings may contribute to our understanding of the mechanistic link between periodontitis and neuroinflammatory diseases.
Abstract
Lysophosphatidic acid (LPA) is a bioactive lipid messenger involved in the pathogenesis of chronic inflammation and various diseases. Recent studies have shown an association between periodontitis and neuroinflammatory diseases such as Alzheimer’s disease, stroke, and multiple sclerosis. However, the mechanistic relationship between periodontitis and neuroinflammatory diseases remains unclear. The current study found that lysophosphatidic acid receptors 1 (LPAR1) and 6 (LPAR6) exhibited increased expression in primary microglia and astrocytes. The primary astrocytes were then treated using medium conditioned to mimic periodontitis through addition of Porphyromonas gingivalis lipopolysaccharides, and an increased nitric oxide (NO) production was observed. Application of conditioned medium from human periodontal ligament stem cells with or without LPAR1 knockdown showed a decrease in the production of NO and expression of inducible nitric oxide synthase and interleukin 1 beta. These findings may contribute to our understanding of the mechanistic link between periodontitis and neuroinflammatory diseases.
참고문헌 (25)
- Elsa Maria Cardoso, "Chronic periodontitis, inflammatory cytokines, and interrelationship with other chronic diseases", Postgraduate Medicine, 130(1), pp.98~104 (2017), Informa UK Limited[DOI]
- Linda L. Humphrey, "Periodontal Disease and Coronary Heart Disease Incidence: A Systematic Review and Meta-analysis", Journal of General Internal Medicine, 23(12), pp.2079~2086 (2008), Springer Science and Business Media LLC[DOI]
- S. Kaur, "Periodontal disease and rheumatoid arthritis: a systematic review", Journal of Dental Research, 92(5), pp.399~408 (2013), SAGE Publications[DOI]
- Daisuke Furutama, "IL-6 Induced by Periodontal Inflammation Causes Neuroinflammation and Disrupts the Blood–Brain Barrier", Brain Sciences, 10(10), pp.679~ (2020), MDPI AG[DOI]
- Xu Wang, "Neuroinflammation changes with periodontal inflammation status during periodontitis in wild‐type mice", Oral Diseases, 27(4), pp.1001~1011 (2020), Wiley[DOI]
- Leonardo Guzman-Martinez, "Neuroinflammation as a Common Feature of Neurodegenerative Disorders", Frontiers in Pharmacology, 10, pp.1008~ (2019), Frontiers Media SA[DOI]
- Michal Schwartz, "Neurological Disease as a Failure of Brain–Immune Crosstalk: The Multiple Faces of Neuroinflammation", Trends in Immunology, 37(10), pp.668~679 (2016), Elsevier BV[DOI]
- Francisco B. Teixeira, "Periodontitis and Alzheimer’s Disease: A Possible Comorbidity between Oral Chronic Inflammatory Condition and Neuroinflammation", Frontiers in Aging Neuroscience, 9, pp.327~ (2017), Frontiers Media SA[DOI]
- María Olimpia Paz Alvarenga, "Is There Any Association Between Neurodegenerative Diseases and Periodontitis? A Systematic Review", Frontiers in Aging Neuroscience, 13, pp.651437~ (2021), Frontiers Media SA[DOI]
- 김동희, "The role of lysophosphatidic acid receptor 1 in inflammatory response induced by lipopolysaccharide from Porphyromonas gingivalis in human periodontal ligament stem cells", International Journal of Oral Biology, 45(2), pp.42~50 (2020), 대한구강생물학회
- Eric Birgbauer, "Lysophosphatidic Acid Signalling in Nervous System Development and Function", NeuroMolecular Medicine, 23(1), pp.68~85 (2020), Springer Science and Business Media LLC[DOI]
- Luiz Henrique Medeiros Geraldo, "Role of lysophosphatidic acid and its receptors in health and disease: novel therapeutic strategies", Signal Transduction and Targeted Therapy, 6(1), pp.45~ (2021), Springer Science and Business Media LLC[DOI]
- Mohammad M. Ahmadzai, "LPAR1 regulates enteric nervous system function through glial signaling and contributes to chronic intestinal pseudo-obstruction", Journal of Clinical Investigation, 132(4), pp.e149464~ (2022), American Society for Clinical Investigation[DOI]
- Mizuki Nagata, "Conditioned Medium from Periodontal Ligament Stem Cells Enhances Periodontal Regeneration", Tissue Engineering Part A, 23(9-10), pp.367~377 (2017), Mary Ann Liebert Inc[DOI]
- Ji Woong Choi, "LPA Receptors: Subtypes and Biological Actions", Annual Review of Pharmacology and Toxicology, 50(1), pp.157~186 (2010), Annual Reviews[DOI]
- Jerold Chun, "Promising Pharmacological Directions in the World of Lysophosphatidic Acid Signaling", Biomolecules & Therapeutics, 23(1), pp.1~11 (2015), 한국응용약물학회
- Hongjiao Xiang, "Lysophosphatidic Acid Receptors: Biochemical and Clinical Implications in Different Diseases", Journal of Cancer, 11(12), pp.3519~3535 (2020), Ivyspring International Publisher[DOI]
- Silvia Anahi Valdés-Rives, "Autotaxin-Lysophosphatidic Acid: From Inflammation to Cancer Development", Mediators of Inflammation, 2017, pp.1~15 (2017), Hindawi Limited[DOI]
- Yona Goldshmit, "LPA receptor expression in the central nervous system in health and following injury", Cell and Tissue Research, 341(1), pp.23~32 (2010), Springer Science and Business Media LLC[DOI]
- Yun C. Yung, "Lysophosphatidic Acid Signaling in the Nervous System", Neuron, 85(4), pp.669~682 (2015), Elsevier BV[DOI]
- AnjaU Bräuer, "Modulation of lysophosphatidic acid (LPA) receptor activity: the key to successful neural regeneration?", Neural Regeneration Research, 15(1), pp.53~54 (2020), Medknow[DOI]
- Tony Frugier, "Modulation of LPA Receptor Expression in the Human Brain Following Neurotrauma", Cellular and Molecular Neurobiology, 31(4), pp.569~577 (2011), Springer Science and Business Media LLC[DOI]
- Anastasia V. Kotova, "Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome", Biomedicines, 9(11), pp.1606~ (2021), MDPI AG[DOI]
- Saori Nonaka, "Secreted gingipains from Porphyromonas gingivalis increase permeability in human cerebral microvascular endothelial cells through intracellular degradation of tight junction proteins", Neurochemistry International, 154, pp.105282~ (2022), Elsevier BV[DOI]
- R.P. Darveau, "Porphyromonas gingivalis as a potential community activist for disease", Journal of Dental Research, 91(9), pp.816~820 (2012), SAGE Publications[DOI]