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Porphyromonas gingivalis lipopolysaccharide의 전신적 투여에 의한 생쥐 뇌에서 염증 유발

Systemic injection of Porphyromonas gingivalis lipopolysaccharide induces inflammation in the mouse brain

이희수|유기연|정보현
대한구강해부학회 (The Korean Academy Of Oral Anatomy)|2020.12|대한구강해부학회지 (The Korean Journal of Oral Anatomy)|제41권 제1호|pp. 55-64|DOI 10.35607/kjoa.41.1.202012.005

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참고문헌

키워드

뇌사이토카인염증Porphyromonas gingivalis지질다당질

초록

치주질환을 일으키는 세균인 Porphyromonas gingivalis 의 lipopolysaccharide (PG-LPS)가 뇌의 혈액뇌장벽(blood-brain-barrier)을 통과 할 가능성이 제기되었지만, 뇌의 신경 염증 유발과 관련된 직접적인 연관성은 명확하게 알려져 있지 않다. 따라서 본 연구에서 PG-LPS가 뇌에서 염증과 관련되어 어떤 영향을 미치는지 확인하고자 하였다. 본 연구에서는 PG-LPS 투여 후 12 시간과 24 시간 후에 면역조직화학 염색을통해 뇌 조직의 변화를 확인 하였다. 면역조직화학 염색 결과, PG-LPS를 투여 한 마우스의 뇌에서 염증관련 인자들이 발현되는 것이 관찰되었다. 이와 같은 결과는 구강 내 치주질환을 유발하는 PG-LPS는 뇌에서신경염증 유발과 밀접한 연관이 있음을 시사한다.

Abstract

Porphyromonas gingivalis is a Gram-negative bacterium that causes periodontal disease in the oral cavity. The possibility that P. gingivalis lipopolysaccharide (PG-LPS) can pass through the blood–brain barrier has been restored; however, it is not known whether it leads to neuroinflammation in the brain. Therefore, in this study, we tried to determine if PG-LPS affects the brain. In this study, inflammatory changes in brain tissues were confirmed through immunohistochemistry 12 h and 24 h after PG-LPS administration. In result, inflammation and cytokine expression were observed in the brain of PG-LPS-administered mice. These results suggest that P. gingivalis can influence inflammation in the brain.

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