Antitumor effects of ophiopogonin D on oral squamous cell carcinoma
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Ophiopogonin D (OPD) is a steroidal glycoside derived from Ophiopogon japonicus , a traditional Chinese medicine with diverse biological activities, including antithrombosis, anti-inflammation, and antitussive effects. To investigate the cellular effects and mechanisms of OPD on oral squamous cell carcinoma, cell viability was explored, and the effects of OPD on cell cycle regulators, apoptotic marker proteins, and key proteins involved in metastasis and signaling pathways were examined by MTT assay and Western blotting in YD38 cells. OPD strongly inhibited cell proliferation and induced caspase-dependent apoptosis of YD38 cells by suppressing the cell cycle and activating caspase-3 and poly ADP ribose polymerase. Additionally, OPD suppressed the expression of vital proteins regulating metastasis and proliferation within the integrin/matrix metalloproteinases/FAK and AKT/PI3K/mTor pathways. Thus, OPD can be a potential treatment candidate for gingival cancer.
Abstract
Ophiopogonin D (OPD) is a steroidal glycoside derived from Ophiopogon japonicus , a traditional Chinese medicine with diverse biological activities, including antithrombosis, anti-inflammation, and antitussive effects. To investigate the cellular effects and mechanisms of OPD on oral squamous cell carcinoma, cell viability was explored, and the effects of OPD on cell cycle regulators, apoptotic marker proteins, and key proteins involved in metastasis and signaling pathways were examined by MTT assay and Western blotting in YD38 cells. OPD strongly inhibited cell proliferation and induced caspase-dependent apoptosis of YD38 cells by suppressing the cell cycle and activating caspase-3 and poly ADP ribose polymerase. Additionally, OPD suppressed the expression of vital proteins regulating metastasis and proliferation within the integrin/matrix metalloproteinases/FAK and AKT/PI3K/mTor pathways. Thus, OPD can be a potential treatment candidate for gingival cancer.
참고문헌 (24)
- Asmin PK, "Occurrence and distribution of cancers with emphasis upon oral cancers in registered oncology institutes of South India-a retrospective study", Indian J Community Med, 49, pp.120~130 (2024)[DOI]
- Badwelan M, "Oral squamous cell carcinoma and concomitant primary tumors, what do we know? A review of the literature", Curr Oncol, 30, pp.3721~3734 (2023)[DOI]
- Tan Y, "Oral squamous cell carcinomas : state of the field and emerging directions", Int J Oral Sci, 15, pp.44~ (2023)[DOI]
- da Silva SD, "Recurrent oral cancer : current and emerging therapeutic approaches", Front Pharmacol, 3, pp.149~ (2012)[DOI]
- Zhang W, "Identification of biological functions and prognostic value of NNMT in oral squamous cell carcinoma", Biomolecules, 12, pp.1487~ (2022)[DOI]
- Chen MH, "Ophiopogon japonicus--a phytochemical, ethnomedicinal and pharmacological review", J Ethnopharmacol, 181, pp.193~213 (2016)[DOI]
- Xu J, "Hypoglycemic effects of MDG-1, a polysaccharide derived from Ophiopogon japonicas, in the ob", ob mouse model of type 2diabetes mellitus, 2011, pp.49, 657~662
- Lan S, "Chemical constituents from the fibrous root of Ophiopogon japonicus, and their effect on tube formation in human myocardial microvascular endothelial cells", Fitoterapia, 85, pp.57~63 (2013)[DOI]
- Chen YT, "Antitumor activity of bacterial exopolysaccharides from the endophyte Bacillus amyloliquefaciens sp. isolated from Ophiopogon japonicus", Oncol Lett, 5, pp.1787~1792 (2013)[DOI]
- Zheng Q, "Influence of sulfation on anti-myocardial ischemic activity of Ophiopogon japonicus polysaccharide", J Asian Nat Prod Res, 11, pp.306~321 (2009)[DOI]
- Wang X, "Xin Su Ning-a review of basic and clinical pharmacology integrated with traditional Chinese medicine antiarrhythmic theory", Front Pharmacol, 12, pp.657484~ (2021)[DOI]
- Newman DJ, "Natural products as sources of new drugs over the period 1981-2002", J Nat Prod, 66, pp.1022~1037 (2003)[DOI]
- Kou J, "Antithrombotic activities of aqueous extract from Radix Ophiopogon japonicus and its two constituents", Biol Pharm Bull, 29, pp.1267~1270 (2006)[DOI]
- Kou J, "Antiinflammatory activities of aqueous extract from Radix Ophiopogon japonicus and its two constituents", Biol Pharm Bull, 28, pp.1234~1238 (2005)[DOI]
- Qian J, "Ophiopogonin D prevents H2O2-induced injury in primary human umbilical vein endothelial cells", J Ethnopharmacol, 128, pp.438~445 (2010)[DOI]
- Ding L, "The roles of cyclin-dependent kinases in cell-cycle progression and therapeutic strategies in human breast cancer", Int J Mol Sci, 21, pp.1960~ (2020)[DOI]
- Casimiro MC, "Cyclins and cell cycle control in cancer and disease", Genes Cancer, 3, pp.649~657 (2012)[DOI]
- Hu Q, "Regulation of the cell cycle by ncRNAs affects the efficiency of CDK4", 6 inhibition, 2023, pp.24, 8939~
- Vitale I, "Apoptotic cell death in disease-current understanding of the NCCD 2023", Cell Death Differ, 30, pp.1097~1154 (2023)[DOI]
- He S, "Proliferation and apoptosis pathways and factors in oral squamous cell carcinoma", Int J Mol Sci, 23, pp.1562~ (2022)[DOI]
- Zang QQ, "Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G2", M, and induces apoptosis in human breast carcinoma MCF-7 cells, 2016, pp.14, 51~59
- Zhang Y, "Ophiopogonin-D suppresses MDA-MB-435 cell adhesion and invasion by inhibiting matrix metalloproteinase-9", Mol Med Rep, 12, pp.1493~1498 (2015)[DOI]
- Zhang YY, "Ophiopogonin D attenuates doxorubicin-induced autophagic cell death by relieving mitochondrial damage in vitro and in vivo", J Pharmacol Exp Ther, 352, pp.166~174 (2015)[DOI]
- Yuan TL, "PI3K pathway alterations in cancer : variations on a theme", Oncogene, 27, pp.5497~5510 (2008)[DOI]