Comparison of oral microbiome diversity between children and young adults in Korea
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The oral microbiome plays a vital role in maintaining oral and overall health and affects immune responses, digestion,and pathogen suppression. While most studies focus on age groups prone to specific conditions, such as dentalcaries in children or periodontal disease in older adults, limited data exist on preschool-aged children and youngadults. This study investigates the composition and diversity of the oral microbiome between these age groupsfor enhanced understanding of a healthy oral microbiome. Microbial samples from the supragingival regions of 41children and 31 young adults in Korea were analyzed using 16S rRNA gene sequencing. Alpha and beta diversitywere assessed, and linear discriminant analysis effect size (LEfSe) identified taxa with significant differences inabundance between the groups. No significant differences in alpha diversity were observed between children andyoung adults however, beta diversity analysis revealed notably compositional differences. At the phylum level,Firmicutes were more abundant in children, whereas Actinobacteria were more prevalent in young adults. Generasuch as Veillonella and Lautropia were more abundant among children, whereas Haemophilus and Rothia were morecommon among young adults. LEfSe analysis identified Veillonella rogosae and Lautropia mirabilis as more abundantin children, whereas Haemophilus parainfluenzae and Rothia dentocariosa were more prevalent in young adults. The observed differences suggest that children’s microbiomes are associated with biofilm development, while youngadults’ microbiomes involve biofilm maturation and immune modulation. These findings highlight the age-related shiftin oral microbiome composition, emphasizing the importance of monitoring these changes to support long-term oralhealth.
Abstract
The oral microbiome plays a vital role in maintaining oral and overall health and affects immune responses, digestion,and pathogen suppression. While most studies focus on age groups prone to specific conditions, such as dentalcaries in children or periodontal disease in older adults, limited data exist on preschool-aged children and youngadults. This study investigates the composition and diversity of the oral microbiome between these age groupsfor enhanced understanding of a healthy oral microbiome. Microbial samples from the supragingival regions of 41children and 31 young adults in Korea were analyzed using 16S rRNA gene sequencing. Alpha and beta diversitywere assessed, and linear discriminant analysis effect size (LEfSe) identified taxa with significant differences inabundance between the groups. No significant differences in alpha diversity were observed between children andyoung adults however, beta diversity analysis revealed notably compositional differences. At the phylum level,Firmicutes were more abundant in children, whereas Actinobacteria were more prevalent in young adults. Generasuch as Veillonella and Lautropia were more abundant among children, whereas Haemophilus and Rothia were morecommon among young adults. LEfSe analysis identified Veillonella rogosae and Lautropia mirabilis as more abundantin children, whereas Haemophilus parainfluenzae and Rothia dentocariosa were more prevalent in young adults. The observed differences suggest that children’s microbiomes are associated with biofilm development, while youngadults’ microbiomes involve biofilm maturation and immune modulation. These findings highlight the age-related shiftin oral microbiome composition, emphasizing the importance of monitoring these changes to support long-term oralhealth.
참고문헌 (54)
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