Supragingival Plaque Microbial Community Analysis of Children with Halitosis
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As one of the most complex human-associated microbial habitats, the oral cavity harbors hundreds of bacteria. Halitosis is a prevalent oral condition that is typically caused by bacteria. The aim of this study was to analyze the microbial communities and predict functional profiles in supragingival plaque from healthy individuals and those with halitosis. Ten preschool children were enrolled in this study; five with halitosis and five without. Supragingival plaque was isolated from each participant and 16S rRNA gene pyrosequencing was used to identify the microbes present. Samples were primarily composed of Actinobacteria, Bacteroidetes, Proteobacteria, Firmicutes, Fusobacteria, and Candidate phylum TM7. The α and β diversity indices did not differ between healthy and halitosis subjects. Fifteen operational taxonomic units (OTUs) were identified with significantly different relative abundances between healthy and halitosis plaques, and included the phylotypes of Prevotella sp., Leptotrichia sp., Actinomyces sp., Porphyromonas sp., Selenomonas sp., Selenomonas noxia, and Capnocytophaga ochracea. We suggest that these OTUs are candidate halitosis-associated pathogens. Functional profiles were predicted using PICRUSt, and nine level-3 KEGG Orthology groups were significantly different. Hub modules of co-occurrence networks implied that microbes in halitosis dental plaque were more highly conserved than microbes of healthy individuals’ plaque. Collectively, our data provide a background for the oral microbiota associated with halitosis from supragingival plaque, and help explain the etiology of halitosis.
Abstract
As one of the most complex human-associated microbial habitats, the oral cavity harbors hundreds of bacteria. Halitosis is a prevalent oral condition that is typically caused by bacteria. The aim of this study was to analyze the microbial communities and predict functional profiles in supragingival plaque from healthy individuals and those with halitosis. Ten preschool children were enrolled in this study; five with halitosis and five without. Supragingival plaque was isolated from each participant and 16S rRNA gene pyrosequencing was used to identify the microbes present. Samples were primarily composed of Actinobacteria, Bacteroidetes, Proteobacteria, Firmicutes, Fusobacteria, and Candidate phylum TM7. The α and β diversity indices did not differ between healthy and halitosis subjects. Fifteen operational taxonomic units (OTUs) were identified with significantly different relative abundances between healthy and halitosis plaques, and included the phylotypes of Prevotella sp., Leptotrichia sp., Actinomyces sp., Porphyromonas sp., Selenomonas sp., Selenomonas noxia, and Capnocytophaga ochracea. We suggest that these OTUs are candidate halitosis-associated pathogens. Functional profiles were predicted using PICRUSt, and nine level-3 KEGG Orthology groups were significantly different. Hub modules of co-occurrence networks implied that microbes in halitosis dental plaque were more highly conserved than microbes of healthy individuals’ plaque. Collectively, our data provide a background for the oral microbiota associated with halitosis from supragingival plaque, and help explain the etiology of halitosis.
참고문헌 (40)
- Aas JA, "Defining the normal bacterial flora of the oral cavity", J. Clin. Microbiol, 43, pp.5721~5732 (2005)
- Abusleme L, "The subgingival microbiome in health and periodontitis and its relationship with community biomass and inflammation", ISME J, 7, pp.1016~1025 (2013)
- Ahn JY, "Oral microbiome profiles: 16S rRNA pyrosequencing and microarray assay comparison", PLoS One, 6, pp.e22788~ (2011)
- Aylikci BU, "Halitosis: from diagnosis to management", J. Nat. Sci. Biol. Med, 4, pp.14~23 (2013)
- Bollen CM, "Halitosis: the multidisciplinary approach", Int. J. Oral Sci, 4, pp.55~63 (2012)
- The human oral microbiome database: a Web accessible resource for investigating oral microbe taxonomic and genomic information, "Chen T", Database (Oxford), ~ (2010)
- Clarridge JE 3rd, "Genotypic diversity of clinical Actinomyces species: phenotype, source, and disease correlation among genospecies", J. Clin. Microbiol, 40, pp.3442~3448 (2002)
- Desai SS, "Capnocytophaga ochracea causing severe sepsis and purpura fulminans in an immunocompetent patient", J. Infect, 54, pp.e107~e109 (2007)
- Dewhirst FE, "The human oral microbiome", J. Bacteriol, 192, pp.5002~5017 (2010)
- Diaz PI, "Using high throughput sequencing to explore the biodiversity in oral bacterial communities", Mol. Oral Microbiol, 27, pp.182~201 (2012)
- Donaldson AC, "Microbiological culture analysis of the tongue anaerobic microflora in subjects with and without halitosis", Oral Dis, 11, pp.61~63 (2005)
- Eribe ER, "Leptotrichia species in human infections", Anaerobe, 14, pp.131~137 (2008)
- Eribe ER, "Genetic diversity of Leptotrichia and description of Leptotrichia goodfellowii sp. nov., Leptotrichia hofstadii sp. nov., Leptotrichia shahii sp. nov. and Leptotrichia wadei sp. nov", Int. J. Syst. Evol. Microbiol, 54, pp.583~592 (2004)
- Hughes FJ, "Oral malodour - a review", Arch. Oral Biol, 53, pp.S1~S7 (2008)
- Human Microbiome Project Consortium, "A framework for human microbiome research", Nature, 486, pp.215~221 (2012)
- Kazor CE, "Diversity of bacterial populations on the tongue dorsa of patients with halitosis and healthy patients", J. Clin. Microbiol, 41, pp.558~563 (2003)
- Kleinberg I, "Oral malodor", Crit. Rev. Oral Biol. Med, 1, pp.247~259 (1990)
- Krespi YP, "The relationship between oral malodor and volatile sulfur compoundproducing bacteria", Otolaryngol. Head Neck Surg, 135, pp.671~676 (2006)
- Langille MG, "Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences", Nat. Biotechnol, 31, pp.814~821 (2013)
- Marsh PD, "Are dental diseases examples of ecological catastrophes?", Microbiology, 149, pp.279~294 (2003)
- Marsh PD, "Dental diseases - are these examples of ecological catastrophes?", Int. J. Dent. Hyg, 4, pp.3~10 (2006)
- Ning J, "Phylogenetic approaches to microbial community classification", Microbiome, 3, pp.47~ (2015)
- Persson S, "The formation of hydrogen sulfide and methyl mercaptan by oral bacteria", Oral Microbiol. Immunol, 5, pp.195~201 (1990)
- Ren W, "Tongue coating and the salivary microbial communities vary in children with halitosis", Sci. Rep, 6, pp.24481~ (2016)
- Rosenstein ED, "Porphyromonas gingivalis, periodontitis and rheumatoid arthritis", Med. Hypotheses, 73, pp.457~458 (2009)
- Samnieng P, "Daily variation of oral malodour and related factors in community-dwelling elderly Thai", Gerodontology, 29, pp.E964~E971 (2012)
- Scully C, "Halitosis(breath odor)", Periodontology 2000, 48, pp.66~75 (2008)
- Segata N, "Metagenomic biomarker discovery and explanation", Genome Biol, 12, pp.R60~ (2011)
- Takeshita T, "Discrimination of the oral microbiota associated with high hydrogen sulfide and methyl mercaptan production", Sci. Rep, 2, pp.215~ (2012)
- Tanaka M, "Contribution of periodontal pathogens on tongue dorsa analyzed with real-time PCR to oral malodor", Microbes Infect, 6, pp.1078~1083 (2004)
- Tanaka S, "The relationship of Prevotella intermedia, Prevotella nigrescens and Prevotella melaninogenica in the supragingival plaque of children, caries and oral malodor", J. Clin. Pediatr. Dent, 32, pp.195~200 (2008)
- Tanner AC, "Anaerobic culture to detect periodontal and caries pathogens", J. Oral Biosci, 57, pp.18~26 (2015)
- Torresyap G, "Relationship between periodontal pocket sulfide levels and subgingival species", J. Clin. Periodontol, 30, pp.1003~1010 (2003)
- Tyrrell KL, "Anaerobic bacteria cultured from the tongue dorsum of subjects with oral malodor", Anaerobe, 9, pp.243~246 (2003)
- Washio J, "Hydrogen sulfide-producing bacteria in tongue biofilm and their relationship with oral malodour", J. Med. Microbiol, 54, pp.889~895 (2005)
- Xu H, "Plaque bacterial microbiome diversity in children younger than 30 months with or without caries prior to eruption of second primary molars", PLoS One, 9, pp.e89269~ (2014)
- Xu X, "Oral cavity contains distinct niches with dynamic microbial communities", Environ. Microbiol, 17, pp.699~710 (2015)
- Yang F, "Microbial basis of oral malodor development in humans", J. Dent. Res, 92, pp.1106~1112 (2013)
- Yang F, "Saliva microbiomes distinguish caries-active from healthy human populations", ISME J, 6, pp.1~10 (2012)
- Zeng B, "The bacterial communities associated with fecal types and body weight of rex rabbits", Sci. Rep, 5, pp.9342~ (2015)