주자독서환의 뇌기능 활성 및 신경세포 보호효과
The Effects of Jujadokseo-hwan on the Activation of Brain and Neuroprotactive Effects
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Objectives This study is designed to investigate the effects of Jujadokseo-hwan on the brain ability and inducing oxidative stresses. Methods We measured the changes of regional cerebral blood flow and mean arterial blood pressure. Then we analyzed histological examination, immunohistochemistric response and anti-oxidant activity of Jujadokseo-hwan. Results 1. Treatment of Jujadokseo-hwan significantly increased regional cerebral blood flow but decreased mean arterial blood pressure. 2. Treatment of Jujadokseo-hwan-induced increase of regional cerebral blood flow was significantly inhibited by pretreatment with indomethacin (1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase. 3. In histological examination through TTC stain, group Ⅰ was no change, but group Ⅱ showed that discolored in the most cortical part. Group Ⅲ showed that decreased discolor in the cortical part. 4. In immunohistochemistric response of BDNF, group Ⅱ showed that lower response effect . Group Ⅲ showed that increase response effect . 5. Treatment of Jujadokseo-hwan increased proliferation rates of Glial cell effectively 6. Treatment of Jujadokseo-hwan accelerated proliferation rates of C6 cells in vitro. In addition, protective effects on cell death induced by paraquat, rotenone and hydrogen peroxide. In addition, activity of SOD were increased by treatment with Jujadokseo-hwan. Conclusions In conclusion, Jujadokseo-hwan can improve of the brain ability , learning ability , memory ability and induce ischemic brain injuries.
Abstract
Objectives This study is designed to investigate the effects of Jujadokseo-hwan on the brain ability and inducing oxidative stresses. Methods We measured the changes of regional cerebral blood flow and mean arterial blood pressure. Then we analyzed histological examination, immunohistochemistric response and anti-oxidant activity of Jujadokseo-hwan. Results 1. Treatment of Jujadokseo-hwan significantly increased regional cerebral blood flow but decreased mean arterial blood pressure. 2. Treatment of Jujadokseo-hwan-induced increase of regional cerebral blood flow was significantly inhibited by pretreatment with indomethacin (1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase. 3. In histological examination through TTC stain, group Ⅰ was no change, but group Ⅱ showed that discolored in the most cortical part. Group Ⅲ showed that decreased discolor in the cortical part. 4. In immunohistochemistric response of BDNF, group Ⅱ showed that lower response effect . Group Ⅲ showed that increase response effect . 5. Treatment of Jujadokseo-hwan increased proliferation rates of Glial cell effectively 6. Treatment of Jujadokseo-hwan accelerated proliferation rates of C6 cells in vitro. In addition, protective effects on cell death induced by paraquat, rotenone and hydrogen peroxide. In addition, activity of SOD were increased by treatment with Jujadokseo-hwan. Conclusions In conclusion, Jujadokseo-hwan can improve of the brain ability , learning ability , memory ability and induce ischemic brain injuries.
참고문헌 (49)
- Raymond D, "신경과학", pp.715~761 (1998), 정담
- 전국한의과대학 병리학교실, "한방병리학", pp.27~31 (2001), 한의문화사
- 조남근, "족삼리 자침이 혈압과 뇌혈류역학에 미치는 영향", 대한침구학회지, 16(4), pp.307~319 (1999)
- 조남근, "용천혈의 자침 및 애구 시술이 혈압과 국소뇌혈류량에 미치는 영향", 대한침구학회지, 15(2), pp.227~236 (1998)
- 배병철, "금석황제내경소문", pp.118~119 (1994), 성보사
- 허준, "동의보감", pp.191~ (1999), 법인문화사
- 허준, "동의보감", pp.190~ (1999), 법인문화사
- 강혁수, "주자독서환의 아밀로이드베타로 유발된 생쥐 알츠하이머모델에 대한 효과", 대한한방내과학회지, 27(1), pp.253~264 (2006)
- 강인탁, "알코올 중독에 대한 주자독서환의 학습능력향상 및 간손상회복 효과", 석사, pp.2007
- Bederson J.B, "Rat middle cerebral artery occlusion: evaluation of the model and development of a neurologic examination", Stroke, 17(3), pp.472~476 (1996)
- Nagasawa H, "Correlation between cerebral blood flow and histologic changes in a new rat model of middle cerebral artery occlusion", Stroke, 20(8), pp.1037~1043 (1989)
- Mari M, "Evaluation of the estrogenic activities of some pesticides and their combinations using MtT", Se cell proliferation assay, 2006, pp.209(5), 413~421
- Lockhart BP, "Conition enhancing or neuroprotective compounds for the treatment of cognitive disorders", why? when? which? Exp Gerontol, 38(1-2), pp.119~128 (2003)
- Simard M, "Memory assessment in studies of cognition-enhancing drugs for Alzheimer's disease", Drugs Aging, 14(3), pp.197~230 (1999)
- 김은선, "뇌질환 치료제Ⅰ, 산업시장분석", pp.25~55 (2006), 한국과학기술정보연구원
- 나영신, "뇌혈관질환에서 SPECT와 PET의 임상적 응용", Nuclear Medicine and Molecular Imaging, 37(1), pp.1~12 (2003), 대한핵의학회
- 대한신경외과학회, "신경외과학", pp.150~156 (1998), 중앙문화사
- 이시진, "본초강목중국의학대계", pp.650~651 (1995), 여강출판사
- 손사막, "비급천금요방. 중국의학대계", pp.896~898 (1995), 여강출판사
- 허준, "동의보감", 법인문화사, pp.487~ (1999)
- 이천, "의학입문", pp.126~ (1994), 의성당
- 황도연, "신증방약합편", pp.376~ (2002), 영림사
- Giacobini E, "From molecular structure to Alzheimer therapy", Jpn J Pharmacol, 74(3), pp.225~241 (1997)
- Giacobini E, "Present and future of Alzheimer therapy", J Neural Transm Suppl, 59, pp.231~242 (2000)
- Bachurin SO, "Medicinal chemistry approaches for the treatment and prevention of Alzheimer's disease", Med Res Rev, 23(1), pp.48~88 (2003)
- Harris RJ, "Changes in extracellular calcium activity in cerebral ischemia", J.Cereb Blood Mrtab, pp.203~209 (2001)
- 김우겸, "인체의 생리", pp.20~47 (1985), 서울대학교 출판부
- Wang Q, "Comparison of the effects of NG-nitro- L-arginine and indomethacin on hypercapnic cerebral blood flow increase in rats", Brain Res, 641(2), pp.257~264 (1994)
- Iwamoto J, "Methylene blue inhibits hypoxic cerebral vasodilation in awake sheep", J.Appl Physiol, 73(6), pp.2226~2232 (1992)
- Okura Y, "Recombinant murine interleukin-12 facilitates induction of cardiac myosin-specific type 1 helper T cells in rats", Circ Res, 82(10), pp.1035~1042 (1998)
- Ginsberg MD, "Neuroprotection for ischemic stroke: past, present and future", Neuro- pharmacology, 55(3), pp.363~389 (2008)
- Buchan AM, "Hypothermia but not the N-methyl-D-aspartate receptor antagonist, MK-801, attenuates neuronal damage in gerbils subjected to transient global ischemia", J Neurosci, 11, pp.1049~1050 (1990)
- Aronowski J, "Graded bioassay for demonstration of brain rescue from experimental acute ischemia in rats", Stroke, 25(11), pp.2235~2240 (1994)
- Goldlust E.J, "Automated measurement of infarct size with scanned images of triphenyltetrazolium chloride-stained rat brains", Stroke, 27(9), pp.1657~1662 (1996)
- Kerege F, "Low brain-derived neurotrophic factor(BDNF) levels in serum of depressed patients probably results from lowered platelet BDNF release unrelated to platelet activity", Biol Psychiatry, 57, pp.1068~1072 (2005)
- Connor B, "The role of neuronal growth factors in neurodegenerative disorders of the human brain", Brain Res Reviews, 27(1), pp.1~39 (1998)
- Legutko B, "Regulation of BDNF expressionin primary neuron culture by LY392098, a novel AMPA receptor potentiator", Neuropharmachology, 40(8), pp.1019~1027 (2001)
- Cotman CW, "Exercise:a behavioral intervention to enhance brain health and plasticity", Trends Neurosci, 25(6), pp.295~301 (2002)
- Gomez-Pinilla F, "A “deficient enviroment” in prenatal life may compromise systems important for cognitive function by affecting BDNF in the hippocampus", Exp Neurol, 192, pp.235~243 (2005)
- Eichenbaum H, "The hippocampus and declaractive memory:cognitive mechanism and neural codes", Behav Brain Res, 127, pp.199~207 (2000)
- Wills BK, "The effect of amifostine, a cytoprotective agent, on paraquat toxicity in mice", J Med Toxicol, 3(1), pp.1~6 (2007)
- Krasowska A, "Cell-protective and antioxidant activity of two groups of synthetic amphiphilic compounds-phenolics and amine N-oxides", Folia Microbiol, 52(6), pp.585~592 (2007)
- Mullens W, "Sodium nitroprusside for advanced low-output heart failure", J Am Coll Cardiol, 52(3), pp.200~207 (2008)
- Molina-Jiménez MF, "Neuroprotective effect of fraxetin and myricetin against rotenone-induced apoptosis in neuroblastoma cells", Brain Res, 1009(1-2), pp.9~16 (2004)
- Zhang JG, "Tirmenstein MA, Fariss MW. Vitamin E succinate protects hepatocytes against the toxic effect of reactive oxygen species generated at mitochondrial complexes I and III by alkylating agents", Chem Biol Interact, 138(3), pp.267~284 (2001)
- Corpas FJ, "The expression of different superoxide dismutase forms is cell-type dependent in olive (Olea europaea L.) leaves", Plant Cell Physio, 47(7), pp.984~994 (2006)
- Corpas FJ, "Peroxisomes as a source of reactive oxygen species and nitric oxide signal molecules in plant cells", Trends Plant, 6(4), pp.145~150 (2001)
- Pompella A, "The changing faces of glutathione, a cellular protagonist", Biochem Pharmacol, 66(8), pp.1499~1503 (2003)
- Pastore A, "Determination of blood total, reduced, and oxidized glutathione in pediatric subjects", Clin. Chem, 47(8), pp.1467~1469 (2003)
