선학초(짚신나물) 경구투여시 항암효과 탐색 및 약물 대사효소의 변화
The Anticancer Effects and Drug Metabolic Enzyme Change by Oral Intake of Agrimonia Pilosa Ledeb
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Objective:This research aimed was to investigate the anti-tumor effect, safety, mechanism and metabolizing enzyme of Agrimonia pilosa LEDEB(APL) in female C57B/L mouse. Methods:At first, to evaluate the anti-tumor activity of APL, we divided into four groups, normal, control, APL100(100㎎/㎏), APL150(150㎎/㎏). LLC obtained American Type Culture Collection was used. LLC had been inoculated to induce tumor. To measure the anti-tumor effect of APL, we calibrate tumor size and weight. To study for mechanism of anti-tumor in APL, we used western blotting and to know metabolizing enzyme in APL we used to real-time PCR. Results:APL100, APL150 inhibited tumor growth after medicine injected. APL did not only induced caspase-dependent apoptosis in LLC-bearing mouse tumor. In APL100, it were decreased 72% in CYP3A11. In APL150, it were decreased 62%, 75% in CYP3A11 and MRP1a respectively. Conclusion:These results suggests that APL has some anti-tumor effects in female C57B/L mouse tumor. APL should be careful use with other drugs related with CYP3A11 or MRP1a.
Abstract
Objective:This research aimed was to investigate the anti-tumor effect, safety, mechanism and metabolizing enzyme of Agrimonia pilosa LEDEB(APL) in female C57B/L mouse. Methods:At first, to evaluate the anti-tumor activity of APL, we divided into four groups, normal, control, APL100(100㎎/㎏), APL150(150㎎/㎏). LLC obtained American Type Culture Collection was used. LLC had been inoculated to induce tumor. To measure the anti-tumor effect of APL, we calibrate tumor size and weight. To study for mechanism of anti-tumor in APL, we used western blotting and to know metabolizing enzyme in APL we used to real-time PCR. Results:APL100, APL150 inhibited tumor growth after medicine injected. APL did not only induced caspase-dependent apoptosis in LLC-bearing mouse tumor. In APL100, it were decreased 72% in CYP3A11. In APL150, it were decreased 62%, 75% in CYP3A11 and MRP1a respectively. Conclusion:These results suggests that APL has some anti-tumor effects in female C57B/L mouse tumor. APL should be careful use with other drugs related with CYP3A11 or MRP1a.
참고문헌 (31)
- 전기택, "2005년 남녀사망자와 사망원인", 젠더리뷰, 3, pp.85~90 (2006)
- 배종면, "상대생존율", 예방의학회지, 37(3), pp.217~219 (2004), 대한예방의학회
- 김성경, "우리나라 암 환자의 개인부담 의료비용", 대한내과학회지, 70(1), pp.61~68 (2006), 대한내과학회
- 한경희, "말기 암 환자 가족 간병인의 삶의 질", 한국호스피스완화의료학회지, 9(1), pp.1~10 (2006), 한국호스피스완화의료학회
- 박신, "Escherichia coli O157:H7의 제어를 위한 선학초 추출물과 NaCl의 병용효과", 한국생물공학회지, 13(2), pp.168~173 (1998)
- 조려화, "선학초 부탄올 추출물의 혈관 이완 효과의 기전에 대한 연구", 생약학회지, 37(2), pp.67~73 (2006), 한국생약학회
- 박경석, "식물병원균에 대한 짚신나물 (선학초) 추출물의 항균활성과 Agrimol B의 동정", 농약과학회지, 10(3), pp.230~236 (2006), 한국농약과학회
- Miyamoto K, "Antitumor activity of methanol extract from roots of Agrimonia pilosa Ledeb", Jpn J Pharmacol, 38(1), pp.9~16 (1985)
- Murayama T, "Agrimoniin, an antitumor tannin of Agrimonia pilosa Ledeb., induces interleukin-1", Anticancer Res., 12(5), pp.1471~1474 (1992)
- Gao K, "Experimental study on decoctum Agrimonia pilosa Ledeb-induced apoptosis in HL-60 cells in vitro", Zhong Yao Cai., 23(9), pp.561~562 (2000)
- 최순자, "선학초(짚신나물)에 의한 in vitro와 in vivo에서의 암세포사멸 기전 탐색", 대한한방내과학회지, 30(2), pp.399~409 (2009), 대한한방내과학회
- 최정원, "선학초 (짚신나물) 복강주사의 항암효과 탐색 및 약물 대사효소의 변화", 대한한의학회지, 30(4), pp.129~141 (2009), 대한한의학회
- 강세찬, "선학초 추출물의 간보호 효과", 생약학회지, 37(1), pp.27~31 (2006), 한국생약학회
- 신민교, "임상본초학", pp.384~386 (1997), 영림사
- 민경진, "선학초 추출물의 항산화 및 항암활성", 한국식품위생안전성학회지, 23(2), pp.149~156 (2008), 한국식품위생안전성학회
- Kloft C, "Population pharmacokinetic-pharmacodynamic model for neutropenia with patient subgroup identification: comparison across anticancer drugs", Clin Cancer Res., 12(18), pp.5481~5490 (2006)
- Ishikawa M, "Pharmacological effects of recombinant human granulocyte colony-stimulating factor modified by polyethylene glycol on anticancer drug- induced neutropenia in mice", Gen Pharmacol, 25(3), pp.533~537 (1994)
- Sakurai M, "Clinical study of recombinant human granulocyte-colony stimulating factor(KW-2228) in pediatric field. 2. Effectiveness on neutropenia associated with administration of anticancer agent and safety", Rinsho Ketsueki, 34(2), pp.119~127 (1993)
- Steller H, "Mechanism and genes of cells suicide", Science, 267, pp.1145~1149 (1995)
- Green, D. R., "Apoptotic pathways:paper wraps stone blunts scissors", Cell, 102, pp.1~4 (2000)
- Meier, P., "Apoptosis in develop A", Nature, 407, pp.796~801 (2000)
- Nunez, G., "Caspases:The proteases of the apoptotic pathway", Oncogene, 17, pp.3237~3245 (1998)
- Kothakota, S., "Caspase-3-generated fragment of gelsolin: Effector of morphological change in apoptosis", Science, 278, pp.294~298 (1997)
- Jonstone, R. W., "Apoptosis:a link between cancer genetics and chemotherapy", Cell, 108, pp.153~164 (2002)
- 임순성, "메톡시화 플라본 유도체 합성과 유도체들이 HT-29 인간 대장암 세포 성장에 미치는 영향", 대한암예방학회지, 11(3), pp.211~217 (2006), 대한암예방학회
- Russel FG, "Multidrug resistance protein 4(MRP4", ABCC4):a versatile efflux transporter for drugs and signalling molecules, 2008, pp.29(4), 200~207
- Deeley RG, "Substrate recognition and transport by multidrug resistance protein 1(ABCC1)", FEBS Lett., 580(4), pp.1103~1111 (2006)
- Van der Kolk DM, "Multidrug resistance protein MRP1, glutathione, and related enzymes. IN: Their importance in acute myeloid leukemia", Adv Exp Med Biol, 457, pp.187~198 (1999)
- Keppler D, "ATP-dependent transport of glutathione S-conjugates by the multidrug resistance protein MRP1 and its apical isoform MRP2", Chem Biol Interact, 111, pp.153~161 (1998)
- Huttunen KM, "Cytochrome P450-activated prodrugs:targeted drug delivery", Curr Med Chem., 15(23), pp.2346~2365 (2008)
- Martignoni M, "Species differences between mouse, rat, dog, monkey and human cytochrome P450-mediated drug metabolism, inhibition and induction", Expert Opin Drug Metab Toxicol, 2(6), pp.875~894 (2006)