Dietary Vitamin E Influences the Levels of Nitric Oxide and Cytokines in Broiler Chickens
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The study investigated the effects of dietary Vitamin E (VE) on nitric oxide (NO) metabolism, immune function and analyzed the correlation between NO free radical and cytokines (IL-2 and IL-6) in broilers. One hundred and fifty 2-week-old broilers were randomly divided into three groups. Control group and lower VE (VE-) group were provided with a basic diet supplemented with 12.55 mg/kg VE and 2.55 mg/kg VE for 30 days, respectively. Higher VE (VE--VE+) group was supplemented with 2.55 mg/kg VE in the first 15 days and then 32.55 mg/kg VE in the next 15 days. Five broilers in each group were then sacrificed on the 5th, 10th, 15th, 20th, 25th and 30th days, respectively, and the content of NO free radical, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and cytokines, IL-2 and IL-6, were measured. The results showed that lower VE could decrease growth performance of broilers while higher VE could increase growth performance and eliminate differences resulted from feeding lower VE dietary in early stages (p<0.05). Compared with the control group, lower VE could increase significantly NO and MDA concentration, and increase IL-2 concentration in serum (p<0.05). Higher VE could significantly increase activities of SOD and glutathione GSH-Px (p<0.05). IL-2 is positively correlated with NO in heart (p<0.05) and IL-6 is negatively correlated with NO in liver (p<0.05) and heart (p<0.01). These results indicate that dietary VE could regulate antioxidant capacity and NO metabolism of broilers and higher VE-supplemented diet could directly decrease production of IL-2.
Abstract
The study investigated the effects of dietary Vitamin E (VE) on nitric oxide (NO) metabolism, immune function and analyzed the correlation between NO free radical and cytokines (IL-2 and IL-6) in broilers. One hundred and fifty 2-week-old broilers were randomly divided into three groups. Control group and lower VE (VE-) group were provided with a basic diet supplemented with 12.55 mg/kg VE and 2.55 mg/kg VE for 30 days, respectively. Higher VE (VE--VE+) group was supplemented with 2.55 mg/kg VE in the first 15 days and then 32.55 mg/kg VE in the next 15 days. Five broilers in each group were then sacrificed on the 5th, 10th, 15th, 20th, 25th and 30th days, respectively, and the content of NO free radical, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and cytokines, IL-2 and IL-6, were measured. The results showed that lower VE could decrease growth performance of broilers while higher VE could increase growth performance and eliminate differences resulted from feeding lower VE dietary in early stages (p<0.05). Compared with the control group, lower VE could increase significantly NO and MDA concentration, and increase IL-2 concentration in serum (p<0.05). Higher VE could significantly increase activities of SOD and glutathione GSH-Px (p<0.05). IL-2 is positively correlated with NO in heart (p<0.05) and IL-6 is negatively correlated with NO in liver (p<0.05) and heart (p<0.01). These results indicate that dietary VE could regulate antioxidant capacity and NO metabolism of broilers and higher VE-supplemented diet could directly decrease production of IL-2.
참고문헌 (27)
- Brigelius-Flohé, R., "The European perspective on vitamin E: current knowledge and future research", Am. J. Clin. Nutr, 76, pp.703~716 (2002)
- Fang, Y. Z, "Free radicals and enzymes - the theoretical basis and its application in biology and medicine" (1989), Science Press
- Fang, Y. Z, "The theories and applications of free radical biology" (2002), Science press
- Geng, Y. J., "Apoptotic death of human leukemic cells induced by vascular cells expressing nitric oxide synthase in response to gammainterferon and tumor necrosis factor-alpha", Cancer Res, 56, pp.866~874 (1996)
- Guo, Q., "ESR and cell culture studies on free radical-scavenging and antioxidant activities of isoflavonoids", Toxicology, 179, pp.171~180 (2002)
- Hafeman, D. G., "Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rats", J. Nutr, 104, pp.580~587 (1974)
- Han, B, "Effects of low temperature on free radical metabolism in broilers with ascites syndrome", Chin. J. Anim. Sci. Vet. Med, 33, pp.327~331 (2002)
- Huang, N, "Advance in gene transcriptional factor NF-κB and its significance in medicine", Sichuan J. Physiol. Sci, 22, pp.2~5 (2000)
- Jin, J, "Research progress in NF-κB studies", Foreign Med. Sci. (Pharm), 27, pp.133~137 (2000)
- Koshland, D. E. J, "The molecular of the year", Science, 258, pp.1861~ (1992)
- Lander, H. M., "Nitric Oxide-stimulated Guanine Nucleotide Exchange on p21ras", J. Biol. Chem, 270, pp.7017~7020 (1995)
- Leshchinsky, T. V, "Relationship between the level of dietary vitamin E and the immune response of broiler chickens", Poult. Sci, 80, pp.1590~1599 (2001)
- Liu, J. C, "Research progress in vitamin E", Chin. J. Hosp. Pharm, 21, pp.116~117 (2001)
- Morrissey, P. A., "Tissue content of α-tocopherol and oxidative stability of broilers receiving dietary α-tocopheryl acetate supplement for various periods pre-slaughter", Br. Poult. Sci, 38, pp.84~88 (1997)
- Peng, H. B., "Induction and stabilization of I kappa B alpha by nitric oxide mediated inhibition of NF-kappa B", J. Biol. Chem, 270, pp.14214~14219 (1995)
- Reiss, U, "Comparison of cytoplasmic superoxide dismutase in liver, heart and brain of aging rats and mice", Biochem. Biophys. Res. Commun, 73, pp.255~262 (1976)
- Placer, Z. A, "Estimation of lipid peroxidation, malindialdehyde in biochemical system", Anal. Biochem, 16, pp.359~367 (1996)
- Rossi, F, "The O2 --forming NADPH oxidase of the phagocytes: nature, mechanisms of activation and function", Biochim. Biophys. Acta, 853, pp.65~89 (1986)
- Roy, R. S, "Ischemia-induced conversion of xanthine dehydrogenase to xanthine oxidase", Fed. Proc, 41, pp.767~772 (1982)
- Stasi, R., "Clinical implications of cytokine and soluble receptor measurements in patients with newly-diagnosed aggressive non-Hodgkin’s lymphoma", Eur. J. Haematol, 54, pp.9~17 (1995)
- Tian, Y., "Properties of natural plant antioxidant in scavenging oxygen free radicals", Food Sci, 26, pp.123~126 (2005)
- Van Snick, J, "Interleukin-6: an overview", Annu. Rev. Immunol, 8, pp.253~278 (1990)
- Vercammen, C, "Prostaglandin E2 inhibits human T-cell proliferation after crosslinking of the CD3-Ti complex by directly affecting T cells at an early step of the activation process", Cell Immunol, 104, pp.24~36 (1987)
- Winterbourn, C. C, "The estimation of red cell superoxide dismutase activity", J. Lab. Clin. Med, 85, pp.337~341 (1975)
- Xu, J. X., "Effects of vitamin E and selenium on the metabolism of different free radicals in broilers", Chin. J. Appl. Ecol, 18, pp.1789~1993 (2007)
- Zhang, S. J, "The roles of vitamin E in free radical metabolism in selenium-deficient animals", Chin. J. Vet, 18, pp.15~17 (1998)
- Zhu, H, "Effect of vitamin E supplement in diet on antioxidant ability of testis in Boer goat", Anim. Reprod. Sci, 117, pp.90~94 (2010)