본문 바로가기
KERD
논문KCI 등재

The effect of calcium propionate on the ruminal bacterial community composition in finishing bulls

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Objective: Manipulating the fermentation to improve the performance of the ruminant has attracted the attention of both farmers and animal scientists. Propionate salt supplementation in the diet could disturb the concentration of propionate and total volatile fatty acids in the rumen. This study was conducted to evaluate the effect of calcium propionate supplementation on the ruminal bacterial community composition in finishing bulls. Methods: Eight finishing bulls were randomly assigned to control group (CONT) and calcium propionate supplementation (PROP) feeding group, with four head per group. The control group was fed normal the total mixed ration (TMR) finishing diet, and PROP group was fed TMR supplemented with 200 g/d calcium propionate. At the end of the 51-day feeding trial, all bulls were slaughtered and rumen fluid was collected from each of the animals. Results: Propionate supplementation had no influence the rumen fermentation parameters (p>0.05). Ruminal bacterial community composition was analyzed by sequencing of hypervariable V3 regions of the 16S rRNA gene. The most abundant phyla were the Firmicutes (60.68%) and Bacteroidetes (23.67%), followed by Tenericutes (4.95%) and TM7 (3.39%). The predominant genera included Succiniclasticum (9.43%), Butyrivibrio (3.74%), Ruminococcus (3.46%) and Prevotella (2.86%). Bacterial community composition in the two groups were highly similar, except the abundance of Tenericutes declined along with the calcium propionate supplementation (p = 0.0078). Conclusion: These data suggest that the ruminal bacterial community composition is nearly unchanged by propionate supplementation in finishing bulls.

Abstract

Objective: Manipulating the fermentation to improve the performance of the ruminant has attracted the attention of both farmers and animal scientists. Propionate salt supplementation in the diet could disturb the concentration of propionate and total volatile fatty acids in the rumen. This study was conducted to evaluate the effect of calcium propionate supplementation on the ruminal bacterial community composition in finishing bulls. Methods: Eight finishing bulls were randomly assigned to control group (CONT) and calcium propionate supplementation (PROP) feeding group, with four head per group. The control group was fed normal the total mixed ration (TMR) finishing diet, and PROP group was fed TMR supplemented with 200 g/d calcium propionate. At the end of the 51-day feeding trial, all bulls were slaughtered and rumen fluid was collected from each of the animals. Results: Propionate supplementation had no influence the rumen fermentation parameters (p>0.05). Ruminal bacterial community composition was analyzed by sequencing of hypervariable V3 regions of the 16S rRNA gene. The most abundant phyla were the Firmicutes (60.68%) and Bacteroidetes (23.67%), followed by Tenericutes (4.95%) and TM7 (3.39%). The predominant genera included Succiniclasticum (9.43%), Butyrivibrio (3.74%), Ruminococcus (3.46%) and Prevotella (2.86%). Bacterial community composition in the two groups were highly similar, except the abundance of Tenericutes declined along with the calcium propionate supplementation (p = 0.0078). Conclusion: These data suggest that the ruminal bacterial community composition is nearly unchanged by propionate supplementation in finishing bulls.

참고문헌 (37)

  1. Nakamura I, "Production of lactic-acid isomers and change of microbial features in the rumen of feedlot cattle", J Anim Physiol Anim Nutr, 61, pp.139~144 (1989)
  2. Aiello RJ, "Volatile fatty acid uptake and propionate metabolism in ruminant hepatocytes", J Dairy Sci, 72, pp.942~949 (1989)
  3. Seal CJ, "Nutritional implications of gastrointestinal and liver metabolism in ruminants", Nutr Res Rev, 6, pp.185~208 (1993)
  4. Rigout S, "Lactational effect of propionic acid and duodenal glucose in cows", J Dairy Sci, 86, pp.243~253 (2003)
  5. Sanchez PH, "Propionibacterium acidipropionici P169 and glucogenic precursors improve rumen fermentation of low-quality forage in beef cattle", J Anim Sci, 92, pp.1738~1746 (2014)
  6. Liu Q, "Effects of calcium propionate on rumen fermentation, urinary excretion of purine derivatives and feed digestibility in steers", J Agric Sci, 147, pp.201~209 (2009)
  7. McNamara JP, "Adipose tissue metabolism and production responses to calcium propionate and chromium propionate", J Dairy Sci, 88, pp.2498~2507 (2005)
  8. Moloney AP, "Growth and carcass composition in sheep offered isoenergetic rations which resulted in different concentrations of ruminal metabolites", Livest Prod Sci, 56, pp.157~164 (1998)
  9. Lee-Rangel HA, "Effect of calcium propionate and sorghum level on lamb performance", Anim Feed Sci Technol, 177, pp.237~241 (2012)
  10. Miettinen H, "Effects of the ratio of ruminal propionate to butyrate on milk yield and blood metabolites in dairy cows", J Dairy Sci, 79, pp.851~861 (1996)
  11. Hurtaud C, "Effects of level and type of energy source (volatile fatty acids or glucose) on milk yield, composition and coagulating properties in dairy cows", Reprod Nutr Dev, 38, pp.315~330 (1998)
  12. Broderick GA, "Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media", J Dairy Sci, 63, pp.64~75 (1980)
  13. Erwin ES, "Volatile fatty acid analyses of blood and rumen fluid by gas chromatography", J Dairy Sci, 44, pp.1768~1771 (1961)
  14. Ding G, "Effect of Saccharomyces cerevisiae on alfalfa nutrient degradation characteristics and rumen microbial populations of steers fed diets with different concentrate-to-forage ratios", J Anim Sci Biotechnol, 5, pp.24~ (2014)
  15. Caporaso JG, "QIIME allows analysis of high-throughput community sequencing data", Nat Methods, 7, pp.335~336 (2010)
  16. Weimer PJ, "Effect of monensin feeding and withdrawal on populations of individual bacterial species in the rumen of lactating dairy cows fed high-starch rations", Appl Microbiol Biotecnol, 80, pp.135~145 (2008)
  17. Stevenson DM, "Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR", Appl Microbiol Biotech, 75, pp.165~174 (2007)
  18. Sheperd AC, "Long-term effects of acetate and propionate on voluntary feed intake by midlactation cows", J Dairy Sci, 81, pp.2240~2250 (1998)
  19. Beiranvand H, "Interactions of alfalfa hay and sodium propionate on dairy calf performance and rumen development", J Dairy Sci, 97, pp.2270~2280 (2014)
  20. Pehrson B, "A comparative study of the effectiveness of calcium propionate and calcium chloride for the prevention of parturient paresis in dairy cows", J Dairy Sci, 81, pp.2011~2016 (1998)
  21. Ferreira LS, "Performance and plasma metabolites of dairy calves fed starter containing sodium butyrate, calcium propionate or sodium monensin", Animal, 5, pp.239~245 (2011)
  22. Kim M, "Investigation of ruminal bacterial diversity in dairy cattle fed supplementary monensin alone and in combination with fat, using pyrosequencing analysis", Can J Microbiol, 60, pp.65~71 (2014)
  23. Zhou Z, "Effects of nitrate on methane production, fermentation, and microbial populations in in vitro ruminal cultures", Bioresour Technol, 103, pp.173~179 (2012)
  24. Mohammed R, "Evaluation of rumen methanogen diversity in cattle fed diets con-taining dry corn distillers grains and condensed tannins using PCR-DGGE and qRT-PCR analyses", Anim Feed Sci Technol, 166-167, pp.122~131 (2011)
  25. Pinloche E, "The effects of a probiotic yeast on the bacterial diversity and population structure in the rumen of cattle", PLoS One, 8, pp.e67824~ (2013)
  26. Zhou Z, "Effects of methanogenic inhibitors on methane production and abundances of methanogens and cellulolytic bacteria in in vitro ruminal cultures", Appl Environ Microbiol, 77, pp.2634~2639 (2011)
  27. Thoetkiattikul H, "Comparative analysis of microbial profiles in cow rumen fed with different dietary fiber by tagged 16S rRNA gene pyrosequencing", Curr Microbiol, 67, pp.130~137 (2013)
  28. Ozutsumi Y, "The effect of protozoa on the composition of rumen bacteria in cattle using 16S rRNA gene clone libraries", Biosci Biotech Bioch, 69, pp.499~506 (2005)
  29. Kim M, "Effect of haylage and monensin supplementation on ruminal bacterial communities of feedlot cattle", Curr Microbiol, 69, pp.169~175 (2014)
  30. Pitta DW, "Bacterial diversity associated with feeding dry forage at different dietary concentrations in the rumen contents of Mehshana buffalo (Bubalus bubalis) using 16S pyrotags", Anaerobe, 25, pp.31~41 (2014)
  31. Kurogi T, "Culture-independent detection of "TM7" bacteria in a streptomycin-resistant acidophilic nitrifying process", Aip Conf Proc, 1585, pp.53~58 (2014)
  32. Yang SL, "Bacterial diversity in the rumen of Gayals (Bos frontalis), Swamp buffaloes (Bubalus bubalis) and Holstein cow as revealed by cloned 16S rRNA gene sequences", Mol Biol Rep, 37, pp.2063~2073 (2010)
  33. Stewart DJ, "Biochemical and biological studies on the lipopolysaccharide of Bacteroides nodosus", Res Vet Sci, 23, pp.319~325 (1977)
  34. Liu JH, "High-grain feeding causes strong shifts in ruminal epithelial bacterial community and expression of Toll-like receptor genes in goats", Front Microbiol, 6 (2015)
  35. Zened A, "Microbial ecology of the rumen evaluated by 454 GS FLX pyrosequencing is affected by starch and oil supplementation of diets", FEMS Microbiol Ecol, 83, pp.504~514 (2013)
  36. Flint HJ, "Polysaccharide utilization by gut bacteria: potential for new insights from genomic analysis", Nat Rev Microbiol, 6, pp.121~131 (2008)
  37. Cotta MA, "Interaction of ruminal bacteria in the production and utilization of maltooligosaccharides from starch", Appl Environ Microbiol, 58, pp.48~54 (1992)

관련 논문AI추천

Effects of Nitrate Addition on Rumen Fermentation, Bacterial Biodiversity and AbundanceLiping Ren, Liping Zhao, Qingxiang Meng, Wei Liu, Xinzhuang Zhang, Yunlong Huo, Zhenming Zhou아세아·태평양축산학회 · Animal Bioscience2015 · 제28권 제10호Ruminal pH pattern, fermentation characteristics and related bacteria in response to dietary live yeast (Saccharomyces cerevisiae) supplementation in beef cattleDong Xianwen, Guan Jiuqiang, Hu Rui, Kang Kun, Luo Xiaolin, Peng Quanhui, Shah Ali Mujtaba, Wanapat Metha, Wang Zhisheng, Zhang Xiangfei아세아·태평양축산학회 · Animal Bioscience2022 · 제35권 제2호Comparison of rectum fecal bacterial community of finishing bulls fed high-concentrate diets with active dry yeast and yeast culture supplementationGao Kai, Geng Chunyin아세아·태평양축산학회 · Animal Bioscience2023 · 제36권 제1호Effects of Italian ryegrass silage-based total mixed ration on rumen fermentation, growth performance, blood metabolites, and bacterial communities of growing Hanwoo heifers구민정, 이상석, 이성실, Arang Son, Chang Dae Jeong, Michelle A. Miguel, Seon-Ho Kim, Sonny C. Ramos, Yong-Il Cho한국축산학회 · 한국축산학회지2023 · 제65권 제5호Mitigating Methane Emissions and Enhancing Rumen Microbial Diversity with Native Rumen Microbes Supplementation김선호, 손아랑, 이상석, 이성대, Michelle Miguel, Mozart Nuzul Aprilliza AM농업생명과학연구원 · 농업생명과학연구2025 · 제59권 제3호Nicotinic acid changes rumen fermentation and apparent nutrient digestibility by regulating rumen microbiota in Xiangzhong black cattleBao Linbin, Liang Huan, Qu Mingren, Song Wanming, Yang Zhuqing, Yu Mingjin, Zhao Xianghui아세아·태평양축산학회 · Animal Bioscience2024 · 제37권 제2호

이 간행물의 다른 논문 (12건)