The effects of low-protein diets and protease supplementation on broiler chickens in a hot and humid tropical environment
2
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
Objective: This experiment was conducted to investigate the effects of dietary crude protein (CP) level and exogenous protease supplementation on growth performance, serum metabolites, carcass traits, small intestinal morphology and endogenous protease activity in broiler chickens reared under a tropical climate. Methods: A total of 480 day-old male broiler chicks were randomly assigned to eight dietary treatments in a 4×2 factorial arrangement. The main effects were CP level (21.0%, 19.7%, 18.5%, or 17.2% from 1 to 21 days and 19.0%, 17.9%, 16.7%, or 15.6% from 22 to 35 days) and protease enzyme supplementation (0 ppm or 500 ppm). All experimental diets were fortified with synthetic feed-grade lysine, methionine, threonine and tryptophan to provide the minimum amino acid recommended levels for Cobb 500. Results: Reducing dietary CP linearly reduced (p<0.05) growth performance, serum albumin, total protein, and carcass traits and increased (p<0.05) serum triglycerides and abdominal fat. There was no consistent effect of reducing dietary CP on morphological parameters of the intestine and on the pancreatic and intestinal endogenous protease activity (p>0.05). Protease supplementation improved (p<0.05) feed conversion ratio, body weight gain, carcass yield and intestinal absorptive surface area. Conclusion: Protease supplementation, as measured by growth performance, intestinal morphology and carcass yield, may alleviate the detrimental effects of low protein diets in broiler chickens.
Abstract
Objective: This experiment was conducted to investigate the effects of dietary crude protein (CP) level and exogenous protease supplementation on growth performance, serum metabolites, carcass traits, small intestinal morphology and endogenous protease activity in broiler chickens reared under a tropical climate. Methods: A total of 480 day-old male broiler chicks were randomly assigned to eight dietary treatments in a 4×2 factorial arrangement. The main effects were CP level (21.0%, 19.7%, 18.5%, or 17.2% from 1 to 21 days and 19.0%, 17.9%, 16.7%, or 15.6% from 22 to 35 days) and protease enzyme supplementation (0 ppm or 500 ppm). All experimental diets were fortified with synthetic feed-grade lysine, methionine, threonine and tryptophan to provide the minimum amino acid recommended levels for Cobb 500. Results: Reducing dietary CP linearly reduced (p<0.05) growth performance, serum albumin, total protein, and carcass traits and increased (p<0.05) serum triglycerides and abdominal fat. There was no consistent effect of reducing dietary CP on morphological parameters of the intestine and on the pancreatic and intestinal endogenous protease activity (p>0.05). Protease supplementation improved (p<0.05) feed conversion ratio, body weight gain, carcass yield and intestinal absorptive surface area. Conclusion: Protease supplementation, as measured by growth performance, intestinal morphology and carcass yield, may alleviate the detrimental effects of low protein diets in broiler chickens.
참고문헌 (26)
- Bregendahl K, "Effect of low protein diets on growth performance and body composition of broiler chicks", Poult Sci, 81, pp.1156~1167 (2002)[DOI]
- Waldroup PW, "Effects of supplementing broiler diets low in crude protein with essential and nonessential amino acids", Poult Sci, 4, pp.425~431 (2005)[DOI]
- Namroud NF, "Effects of fortifying low crude protein diet with crystalline amino acids on performance, blood ammonia level, and excreta characteristics of broiler chicks", Poult Sci, 87, pp.2250~2258 (2008)[DOI]
- Laudadio V, "Productive performance and histological features of intestinal mucosa of broiler chickens fed different dietary protein levels", Poult Sci, 91, pp.265~270 (2012)[DOI]
- Awad EA, "Amino acids fortification of low-protein diet for broilers under tropical climate. 2. Nonessential amino acids and increasing essential amino acids", Italian J Anim Sci, 13, pp.631~636 (2014)
- Awad EA, "Effects of feeding male and female broiler chickens on low-protein diets fortified with different dietary glycine levels under the hot and humid tropical climate", Italian J Anim Sci, 16, pp.453~461 (2017)[DOI]
- Temim S, "Effects of chronic heat exposure and protein intake on growth performance, nitrogen retention and muscle development in broiler chickens", Reprod Nutr Dev, 39, pp.145~156 (1999)[DOI]
- Cowieson AJ, "Effect of exogenous enzymes in maize based diets varying in nutrient density for young broilers: growth performance and digestibility of energy, minerals and amino acids", Br Poult Sci, 49, pp.37~44 (2008)[DOI]
- Ding XM, "Effects of dietary crude protein levels and exogenous protease on performance, nutrient digestibility, trypsin activity and intestinal morphology in broilers", Livest Sci, 193, pp.26~31 (2016)[DOI]
- Mahmood T, "Effect of supplementing exogenous protease in low protein poultry by-product meal based diets on growth performance and nutrient digestibility in broilers", Anim Feed Sci Technol, 228, pp.23~31 (2017)[DOI]
- Angel C, "Effects of a monocomponent protease on performance and protein utilization in 7-to 22-day-old broiler chickens", Poult Sci, 90, pp.2281~2286 (2011)[DOI]
- Law FL, "Nutrient digestibility of broiler chickens fed on a low-protein diet supplemented with mono-component proteases", Eur Poult Sci, 79, pp.107~ (2015)
- Mahagna M, "Effect of age and exogenous amylase and protease on development of the digestive tract, pancreatic enzyme activities and digestibility of nutrients in young meat-type chicks", Reprod Nutr, 35, pp.201~212 (1995)[DOI]
- Yegani M, "Effects of corn source and exogenous enzymes on growth performance and nutrient digestibility in broiler chickens", Poult Sci, 92, pp.1208~1220 (2013)[DOI]
- Cowieson A, "Interactive effects of dietary protein concentration, a mono-component exogenous protease and ascorbic acid on broiler performance, nutritional status and gut health", Anim Produ Sci, 57, pp.1058~1068 (2016)
- Kamel NF, "Effects of a monocomponent protease on performance parameters and protein digestibility in broiler chickens", Agric Agric Sci Procedia, 6, pp.216~225 (2015)[DOI]
- Xu X, "Effects of coated proteases on the performance, nutrient retention, gut morphology and carcass traits of broilers fed corn or sorghum based diets supplemented with soybean meal", Anim Feed Sci Technol, 223, pp.119~127 (2017)[DOI]
- Marchini CFP, "Physiology, cell dynamics of small intestinal mucosa, and performance of broiler chickens under heat stress: a review", Rev Colom Cienc Pecua, 29, pp.159~168 (2016)
- Jin L, "Digestive and bacterial enzyme activities in broilers fed diets supplemented with Lactobacillus cultures", Poult Sci, 79, pp.886~891 (2000)[DOI]
- AOAC (Association of Official Analytical Chemists) international, "Official methods of analysis. 17th ed. Association of Official Analytical Chemist" (2000)
- Sklan D, "Interactions between dietary crude protein and essential amino acid intake on performance in broilers", Br Poult Sci, 43, pp.442~449 (2002)[DOI]
- Rehman Z, "Influence of lowprotein and low-amino acid diets with different sources of protease on performance, carcasses and nitrogen retention of broiler chickens", Anim Prod Sci (2017)[DOI]
- Lauri A, "Blood chemistry, cytology and body condition in adult northern goshawks", J Raptor Res, 37, pp.299~306 (2003)
- Abudabos AM, "Effect of enzyme supplementation to normal and low density broiler diets based on corn-soybean meal", Asian J Anim Vet Adv, 7, pp.139~148 (2012)[DOI]
- Lensing M, "The threonine requirement of broiler chickens during subclinical intestinal infection", Proceedings of the 16th European Symposium on Poultry Nutrition 2007, pp.343~346 (2007), World Poultry Science Association
- Ghazi S, "The potential for the improvement in the nutritive value of soya-bean meal by different proteases in broiler chicks and broiler cockerels", Br Poult Sci, 43, pp.70~77 (2002)[DOI]