Endogenous enzyme activities and tibia bone development of broiler chickens fed wheat-based diets supplemented with xylanase, β-glucanase and phytase
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Objective: This study assessed the effect of different levels of xylanase, β-glucanase and phytase on intestinal enzyme activities and tibia bone development in broiler chickens fed wheat-based diets.Methods: Twelve experimental diets were formulated using a 3×2×2 factorial design (three doses of phytase and two doses of both xylanase and β-glucanase) and offered to 648 dayold Ross 308 male chicks having 6 replicates groups with 9 birds per replicate and lasted for 35 days.Results: An interaction between the enzymes products improved (p<0.01) the activity of chymotrypsin. Protein content at d 10 was highest (p<0.001) with addition of phytase while general proteolytic activity (GPA) (p<0.02) and lipase activity (p<0.001) were decreased. At d 24, there were improvements in protein content (p<0.01) and lipase (p<0.04) with supplementation of superdose phytase. Addition of superdose phytase decreased in chymotrypsin (p<0.02), trypsin (p<0.01) and GPA (p<0.001). The optimum dose of xylanase decreased the chymotrypsin activity (p = 0.05), while the GPA (p<0.001) was increased with the optimum level of β-glucanase. Superdose phytase supplementation at d 10 improved maltase (p = 0.05), sucrase (p<0.001) and alkaline phosphatase (p<0.001) activities in the jejunum while aminopeptidase activity was highest (p<0.005) with the low level of phytase. Protein content of jejunum mucosa was bigger (p<0.001) in birds fed superdose phytase while maltase activity (p<0.001) at d 24 was reduced by this treatment. Sucrase (p<0.04) and aminopeptidase activities (p<0.001) improved when diets supplemented with low levels of phytase. Tibia bone breaking strength was highest (p<0.04) with addition of low level of superdose phytase or optimum level of β-glucanase. Bone dry matter content decreased (p<0.04) when diets supplemented with phytase.Conclusion: From the results obtained in this study, supplementation of superdose phytase was the most effective, however, the cost-benefit analysis of the use of such a dose needs to be evaluated.
Abstract
Objective: This study assessed the effect of different levels of xylanase, β-glucanase and phytase on intestinal enzyme activities and tibia bone development in broiler chickens fed wheat-based diets. Methods: Twelve experimental diets were formulated using a 3×2×2 factorial design (three doses of phytase and two doses of both xylanase and β-glucanase) and offered to 648 dayold Ross 308 male chicks having 6 replicates groups with 9 birds per replicate and lasted for 35 days. Results: An interaction between the enzymes products improved (p<0.01) the activity of chymotrypsin. Protein content at d 10 was highest (p<0.001) with addition of phytase while general proteolytic activity (GPA) (p<0.02) and lipase activity (p<0.001) were decreased. At d 24, there were improvements in protein content (p<0.01) and lipase (p<0.04) with supplementation of superdose phytase. Addition of superdose phytase decreased in chymotrypsin (p<0.02), trypsin (p<0.01) and GPA (p<0.001). The optimum dose of xylanase decreased the chymotrypsin activity (p = 0.05), while the GPA (p<0.001) was increased with the optimum level of β-glucanase. Superdose phytase supplementation at d 10 improved maltase (p = 0.05), sucrase (p<0.001) and alkaline phosphatase (p<0.001) activities in the jejunum while aminopeptidase activity was highest (p<0.005) with the low level of phytase. Protein content of jejunum mucosa was bigger (p<0.001) in birds fed superdose phytase while maltase activity (p<0.001) at d 24 was reduced by this treatment. Sucrase (p<0.04) and aminopeptidase activities (p<0.001) improved when diets supplemented with low levels of phytase. Tibia bone breaking strength was highest (p<0.04) with addition of low level of superdose phytase or optimum level of β-glucanase. Bone dry matter content decreased (p<0.04) when diets supplemented with phytase. Conclusion: From the results obtained in this study, supplementation of superdose phytase was the most effective, however, the cost-benefit analysis of the use of such a dose needs to be evaluated.
참고문헌 (41)
- Maenz DD, "Phytase activity in the small intestinal brush border membrane of the chicken", Poult Sci, 77, pp.557~563 (1998)[DOI]
- Bedford MR, "Exogenous enzymes in monogastric nutrition — their current value and future benefits", Anim Feed Sci Technol, 86, pp.1~13 (2000)[DOI]
- Bedford MR, "Exogenous enzymes for pigs and poultry", Nutr Res Rev, 11, pp.91~114 (1998)[DOI]
- Cowieson AJ, "Factors that affect the nutritional value of maize for broilers", Anim Feed Sci Technol, 119, pp.293~305 (2005)[DOI]
- Zhengyu Jiang, "Effects of Different Levels of Supplementary Alpha-amylase on Digestive Enzyme Activities and Pancreatic Amylase mRNA Expression of Young Broilers", Animal Bioscience, 21(1), pp.97~102 (2008), 아세아·태평양축산학회
- Angel CR, "Effects of a monocomponent protease on performance and protein utilization in 7-to 22-day-old broiler chickens", Poult Sci, 90, pp.2281~2286 (2011)[DOI]
- Kalmendal R, "Effects of a xylanase and protease, individually or in combination, and an ionophore coccidiostat on performance, nutrient utilization, and intestinal morphology in broiler chickens fed a wheat-soybean meal-based diet", Poult Sci, 91, pp.1387~1393 (2012)[DOI]
- Yegani M, "Effects of corn source and exogenous enzymes on growth performance and nutrient digestibility in broiler chickens", Poult Sci, 92, pp.1208~1220 (2013)[DOI]
- Ferket PR, "Effect of severity of early protein restriction on large turkey toms. : 1. Performance characteristics and leg weakness", Poult Sci, 68, pp.676~686 (1989)[DOI]
- Hester PY, "The effect of restrictive and compensatory growth on the incidence of leg abnormalities and performance of commercial male turkeys", Poult Sci, 69, pp.1731~1742 (1990)[DOI]
- Pintar J, "Effects of supplemental phytase on nutrient excretion and retention in broilers fed different cereal based diets", Czech Anim Sci, 50, pp.40~46 (2005)[DOI]
- Shelton JL, "Effects of phytase addition with or without a trace mineral premix on growth performance, bone response variables, and tissue mineral concentrations in commercial broilers", J Appl Poult Res, 15, pp.94~102 (2006)[DOI]
- Yi Z, "Supplemental microbial phytase improves zinc utilization in broilers", Poult Sci, 75, pp.540~546 (1996)[DOI]
- Swiatkiewicz S, "Bioavailability of zinc from inorganic and organic sources in broiler chickens fed diets with different levels of non-starch polysaccharides", Ann Anim Sci, 1, pp.99~111 (2001)
- Viveros A, "Effects of microbial phytase supplementation on mineral utilization and serum enzyme activities in broiler chicks fed different levels of phosphorus", Poult Sci, 81, pp.1172~1183 (2002)[DOI]
- Żyła K, "Simultaneous application of phytase and xylanase to broiler feeds based on wheat : feeding experiment with growing broilers", J Sci Food Agric, 79, pp.1841~1848 (1999)[DOI]
- Aviagen W, "Ross 308 broiler nutrition specifications", Aviagen Group, ~ (2014)
- Shirazi-Beechey SP, "Postnatal development of lamb intestinal digestive enzymes is not regulated by diet", J Physiol, 437, pp.691~698 (1991)[DOI]
- Nitsan Z, "The effects of force-feeding on enzymes of the liver, kidney, pancreas and digestive tract of chicks", Br J Nutr, 32, pp.241~247 (1974)[DOI]
- Iji PA, "Body and intestinal growth of broiler chicks on a commercial starter diet. 2. Development and characteristics of intestinal enzymes", Br Poult Sci, 42, pp.514~522 (2001)[DOI]
- Holdsworth ES, "The effect of vitamin D on enzyme activities in the mucosal cells of the chick small intestine", J Membr Biol, 3, pp.43~53 (1970)[DOI]
- Serviere-Zaragoza E, "Protein-hydrolyzing enzymes in the digestive systems of the adult Mexican blue abalone, Haliotis fulgens(Gastropoda)", Aquaculture, 157, pp.325~336 (1997)[DOI]
- Bradford MM, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal Biochem, 72, pp.248~254 (1976)[DOI]
- Short FJ, "Determination of titanium dioxide added as an inert marker in chicken digestibility studies", Anim Feed Sci Technol, 59, pp.215~221 (1996)[DOI]
- Minitab, "MINITAB release 17 : statistical software for windows", Minitab Inc, ~ (2014)
- Y. L. Peng, "Effects of Microbial Phytase Replacing Partial Inorganic Phosphorus Supplementation and Xylanase on the Growth Performance and Nutrient Digestibility in Broilers Fed Wheat-based Diets", Animal Bioscience, 16(2), pp.239~247 (2003), 아세아·태평양축산학회
- Plumstead PW, "Interaction of calcium and phytate in broiler diets. 1. Effects on apparent prececal digestibility and retention of phosphorus", Poult Sci, 87, pp.449~458 (2008)[DOI]
- Cowieson AJ, "The effects of phytase and phytic acid on the loss of endogenous amino acids and minerals from broiler chickens", Br Poult Sci, 45, pp.101~108 (2004)[DOI]
- Silversides FG, "The effect of phytase enzyme and level on nutrient extraction by broilers", Poult Sci, 83, pp.985~989 (2004)[DOI]
- Fuente JM, "Effect of storage time and dietary enzyme on the metabolizable energy and digesta viscosity of barley-based diets for poultry", Poult Sci, 77, pp.90~97 (1998)[DOI]
- Pinheiro DF, "Effect of early feed restriction and enzyme supplementation on digestive enzyme activities in broilers", Poult Sci, 83, pp.1544~1550 (2004)[DOI]
- Yusoff NM, "Preliminary investigation of myo-inositol phosphates produced by ASUIA279phytase on MCF-7 cancer cells", IIUM Eng J, 12, pp.141~151 (2011)[DOI]
- Wu YB, "Influence of exogenous enzyme supplementation on energy utilisation and nutrient digestibility of cereals for broilers", J Sci Food Agric, 84, pp.1817~1822 (2004)[DOI]
- Rutherfurd SM, "Effect of microbial phytase on ileal digestibility of phytate phosphorus, total phosphorus, and amino acids in a low-phos-phorus diet for broilers", Poult Sci, 83, pp.61~68 (2004)[DOI]
- Ravindran V, "Microbial phytase improves performance, apparent metabolizable energy, and ileal amino acid digestibility of broilers fed a lysine-deficient diet", Poult Sci, 80, pp.338~344 (2001)[DOI]
- Liu D, "Xylanase supplementation to a wheatbased diet alleviated the intestinal mucosal barrier impairment of broiler chickens challenged by Clostridium perfringens", Avian Pathol, 41, pp.291~298 (2012)[DOI]
- Munyaka PM, "Impact of combined β-glucanase and xylanase enzymes on growth performance, nutrients utilization and gut microbiota in broiler chickens fed corn or wheat-based diets", Poult Sci, 95, pp.528~540 (2016)[DOI]
- Brenes A, "Effect of enzyme supplementation on the performance and digestive tract size of broiler chickens fed wheat-and barley-based diets", Poult Sci, 72, pp.1731~1739 (1993)[DOI]
- Kies AK, "A review of the antinutritional effects of phytic acid on protein utilisation by broilers", Proceedings of the Australian Poultry Science Symposium, pp.128~131 (1998)
- Tang HO, "Effects of a thermostable phytase on the growth performance and bone mineralization of broilers", J Appl Poult Res, 21, pp.476~483 (2012)[DOI]
- Knott L, "Collagen cross-links in mineralizing tissues : a review of their chemistry, function, and clinical relevance", Bone, 22, pp.181~187 (1998)[DOI]
