NCAM140 and pCREB Expression after Tianeptine Treatment of SH-SY5Y Cells
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objective Antidepressants modulate neuronal plasticity. Tianeptine, an atypical antidepressant, might be involved in the restoration of neuronal plasticity; it primarily enhances the synaptic reuptake of serotonin. NCAM140 is involved in neuronal development processes, synaptogenesis and synaptic plasticity. We investigated the effect of tianeptine on the expression of NCAM140 and its downstream signaling molecule in the human neuroblastoma cell line SH-SY5Y. Methods NCAM protein expression was measured in human neuroblastoma SH-SY5Y cells that were cultivated in serum-free media and treated with 0, 10, or 20 μM tianeptine for 6, 24, or 72 hours. NCAM140 expression in the tianeptine treatment group was confirmed by Western blot, and quantified through measurement of band intensity by absorbance. CREB and pCREB expression was identified after treatment with 20 μM tianeptine for 6, 24, and 72 hours by Western blot. Results Compared to cells treated for 6 hours, cells treated with 0 or 10 μM tianeptine for 72 hours showed a significant increase in NCAM140 expression and cells treated with 20 μM tianeptine showed a significant increase after 24 and 72 hours. The pCREB level in cells treated with 20 μM tianeptine increased in time-dependent manner. Conclusion Our findings indicated that the tianeptine antidepressant effect may occur by induction of NCAM140 expression and CREB phosphorylation.
Abstract
Objective Antidepressants modulate neuronal plasticity. Tianeptine, an atypical antidepressant, might be involved in the restoration of neuronal plasticity; it primarily enhances the synaptic reuptake of serotonin. NCAM140 is involved in neuronal development processes, synaptogenesis and synaptic plasticity. We investigated the effect of tianeptine on the expression of NCAM140 and its downstream signaling molecule in the human neuroblastoma cell line SH-SY5Y. Methods NCAM protein expression was measured in human neuroblastoma SH-SY5Y cells that were cultivated in serum-free media and treated with 0, 10, or 20 μM tianeptine for 6, 24, or 72 hours. NCAM140 expression in the tianeptine treatment group was confirmed by Western blot, and quantified through measurement of band intensity by absorbance. CREB and pCREB expression was identified after treatment with 20 μM tianeptine for 6, 24, and 72 hours by Western blot. Results Compared to cells treated for 6 hours, cells treated with 0 or 10 μM tianeptine for 72 hours showed a significant increase in NCAM140 expression and cells treated with 20 μM tianeptine showed a significant increase after 24 and 72 hours. The pCREB level in cells treated with 20 μM tianeptine increased in time-dependent manner. Conclusion Our findings indicated that the tianeptine antidepressant effect may occur by induction of NCAM140 expression and CREB phosphorylation.
참고문헌 (38)
- Kessler RC, "The epidemiology of major depressive disorder: results from the National Comorbidity Survey Replication (NCS-R)", JAMA, 289, pp.3095~3105 (2003)
- Stahl SM, "SNRIs: their pharmacology, clinical efficacy, and tolerability in comparison with other classes of antidepressants", CNS Spectr, 10, pp.732~747 (2005)
- Uzbay TI, "Tianeptine: potential influences on neuroplasticity and novel pharmacological effects", Prog Neuropsychopharmacol Biol Psychiatry, 32, pp.915~924 (2008)
- Mennini T, "Tianeptine, a selective enhancer of serotonin uptake in rat brain", Naunyn Schmiedebergs Arch Pharmacol, 336, pp.478~482 (1987)
- McEwen BS, "Prevention of stress-induced morphological and cognitive consequences", Euro Neuropsychopharmacol, 7(Suppl 3), pp.S323~S328 (1997)
- Conrad CD, "Chronic stress impairs rat spatial memory on the Y maze, and this effect is blocked by tianeptine pretreatment", Behav Neurosci, 110, pp.1321~1334 (1996)
- Kasper S, "A meta-analysis of randomized controlled trials of tianeptine versus SSRI in the short-term treatment of depression", Eur Psychiatry, 17(Suppl 3), pp.331~340 (2002)
- Beracochea D, "Comparison of the effects of tianeptine and paroxetine on amnesic deficits induced by prolonged alcohol drinking in mice", Encephale, 20, pp.13~16 (1994)
- Jaffard R, "Effects of tianeptine on learning and memory in mice. Improvement of impairments induced by chronic alcoholism and brain aging", Presse Med, 20, pp.1812~1816 (1991)
- Magarinos AM, "Effects of antidepressants and benzodiazepine treatments on the dendritic structure of CA3 pyramidal neurons after chronic stress", Euro J Pharmacol, 371, pp.113~122 (1999)
- Sairanen M, "Brain-derived neurotrophic factor and antidepressant drugs have different but coordinated effects on neuronal turnover, proliferation, and survival in the adult dentate gyrus", J Neurosci, 25, pp.1089~1094 (2005)
- Castrén E, "Is mood chemistry?", Nat Rev Neurosci, 6, pp.241~246 (2005)
- Fujioka T, "Activation of cAMP signaling facilitates the morphological maturation of newborn neurons in adult hippocampus", J Neurosci, 24, pp.319~328 (2004)
- Santarelli L, "Requirement of hippocampal neurogenesis for the behavioral effects of antidepressants", Science, 301, pp.805~809 (2003)
- Schmid RS, "L1 and NCAM adhesion molecules as signaling coreceptors in neuronal migration and process outgrowth", Curr Opin Neurobiol, 18, pp.245~250 (2008)
- Schachner M, "Neural recognition molecules and synaptic plasticity", Curr Opin Cell Biol, 9, pp.627~634 (1997)
- Kiss JZ, "The role of neural cell adhesion molecules in plasticity and repair", Brain Res Brain Res Rev, 36, pp.175~184 (2001)
- Touyarot K, "Chronic restraint stress induces an isoform-specific regulation on the neural cell adhesion molecule in the hippocampus", Neural Plast, 9, pp.147~159 (2002)
- Feng Z, "Neuronal differentiation and protection from nitric oxide-induced apoptosis require c-Jun-dependent expression of NCAM140", Mol Cell Biol, 22, pp.5357~5366 (2002)
- 최미란, "흰쥐 C6 신경교종 세포에서 Fluoxetine을 장기간 처리 시 발현이 조절되는 유전자의 규명", 대한정신약물학회지, 15(4), pp.457~467 (2004), 대한정신약물학회
- 최미란, "Fluoxetine Increases the Expression of NCAM140 and pCREB in Rat C6 Glioma Cells", PSYCHIATRY INVESTIGATION, 9(2), pp.180~186 (2012), 대한신경정신의학회
- Plaisant F, "Neuroprotective properties of tianeptine: interactions with cytokines", Neuropharmacology, 44, pp.801~809 (2003)
- Sairanen M, "Chronic antidepressant treatment selectively increases expression of plasticity-related proteins in the hippocampus and medial prefrontal cortex of the rat", Neuroscience, 144, pp.368~374 (2007)
- Fang S, "Chronic effects of venlafaxine on synaptophysin and neuronal cell adhesion molecule in the hippocampus of cerebral ischemic mice", Biochem Cell Biol, 88, pp.655~663 (2010)
- Sandi C, "A model for the involvement of neural cell adhesion molecules in stress-related mood disorders", Neuroendocrinology, 85, pp.158~176 (2007)
- Cryan JF, "Assessing antidepressant activity in rodents:recent developments and future needs", Trends Pharmacol Sci, 23, pp.238~245 (2002)
- Berton O, "New approaches to antidepressant drug discovery:beyond monoamines", Nat Rev Neurosci, 7, pp.137~151 (2006)
- Pittenger C, "Stress, depression, and neuroplasticity: a convergence of mechanisms", Neuropsychopharmacology, 33, pp.88~109 (2008)
- Nestler EJ, "Neurobiology of depression", Neuron, 34, pp.13~25 (2002)
- Norrholm SD, "Altered dendritic spine density in animal models of depression and in response to antidepressant treatment", Synapse, 42, pp.151~163 (2001)
- McEwen BS, "Stress and hippocampal plasticity", Ann Rev Neurosci, 22, pp.105~122 (1999)
- Kolkova K, "Neural cell adhesion molecule-stimulated neurite outgrowth depends on activation of protein kinase C and the Ras-mitogen-activated protein kinase pathway", J Neurosci, 20, pp.2238~2246 (2000)
- D’Sa C, "Antidepressants and neuroplasticity", Bipolar Disord, 4, pp.183~194 (2002)
- Zarate CA Jr, "Regulation of cellular plasticity cascades in the pathophysiology and treatment of mood disorders: role of the glutamatergic system", Ann N Y Acad Sci, 1003, pp.273~291 (2003)
- Mark LP, "Pictorial review of glutamate excitotoxicity: fundamental concepts for neuroimaging", AJNR Am J Neuroradiol, 22, pp.1813~1824 (2001)
- Kole MHP, "The antidepressant tianeptine persistently modulates glutamate receptor currents of the hippocampal CA3 commissural associational synapse in chronically stressed rats", European J Neurosci, 16, pp.807~816 (2002)
- Budziszewska B, "Antidepressant drugs inhibit glucocorticoid receptor-mediated gene transcription - a possible mechanism", Br J Pharmacol, 130, pp.1385~1393 (2000)
- Holst BD, "Allosteric modulation of AMPA-type glutamate receptors increases activity of the promoter for the neural cell adhesion molecule, N-CAM", Proc Natl Acad Sci U S A, 95, pp.2597~2602 (1998)
