Effects of quercetin and coated sodium butyrate dietary supplementation in diquat-challenged pullets
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Objective: This study was designed to investigate the hypothesis that dietary quercetin (QUE) and coated sodium butyrate (SB) supplementation alleviate oxidative stress in the small intestine of diquat (DIQ)-challenged pullets. Methods: A total of 200 13-week-old pullets were divided into four groups: the control group (CON), the DIQ group, the QUE group, and the coated SB group, and injected intraperitoneally with either saline (CON) or diquat (DIQ, QUE, and SB) to induce oxidative stress on day 0. Results: On the first day, the malondialdehyde and superoxide dismutase (SOD) concentrations in the SB group were significantly different from those in the DIQ and QUE groups (p<0.05), and dietary supplementation with SB increased serum glutathione peroxidase (GSH-PX) levels compared with the DIQ group (p<0.05). Quercetin and SB increased the levels of CLAUDIN-1 and zonula occludens-1 (ZO-1) in the jejunum. On the tenth day of treatment, QUE attenuated the decrease in GSH-PX levels compared to those of the CON group (p<0.05), while SB increased SOD, GSH-PX, and total antioxidant capacity levels compared to those of the DIQ group. Nuclear factor erythroid 2-related factor 2 (NRF2) and heme oxygenase-1 (HO-1) mRNA levels in the QUE and SB groups increased (p<0.05) and CLAUDIN-1 mRNA levels in the QUE and SB groups were upregulated compared to those in the DIQ group ileum tissue. Conclusion: Supplementation of QUE and SB demonstrated the ability to relieve oxidative stress in pullets post DIQ-injection with a time-dependent manner and QUE and SB may be potential antioxidant additives for relieving oxidative stress and protecting the intestinal barrier of pullets.
Abstract
Objective: This study was designed to investigate the hypothesis that dietary quercetin (QUE) and coated sodium butyrate (SB) supplementation alleviate oxidative stress in the small intestine of diquat (DIQ)-challenged pullets. Methods: A total of 200 13-week-old pullets were divided into four groups: the control group (CON), the DIQ group, the QUE group, and the coated SB group, and injected intraperitoneally with either saline (CON) or diquat (DIQ, QUE, and SB) to induce oxidative stress on day 0. Results: On the first day, the malondialdehyde and superoxide dismutase (SOD) concentrations in the SB group were significantly different from those in the DIQ and QUE groups (p<0.05), and dietary supplementation with SB increased serum glutathione peroxidase (GSH-PX) levels compared with the DIQ group (p<0.05). Quercetin and SB increased the levels of CLAUDIN-1 and zonula occludens-1 (ZO-1) in the jejunum. On the tenth day of treatment, QUE attenuated the decrease in GSH-PX levels compared to those of the CON group (p<0.05), while SB increased SOD, GSH-PX, and total antioxidant capacity levels compared to those of the DIQ group. Nuclear factor erythroid 2-related factor 2 (NRF2) and heme oxygenase-1 (HO-1) mRNA levels in the QUE and SB groups increased (p<0.05) and CLAUDIN-1 mRNA levels in the QUE and SB groups were upregulated compared to those in the DIQ group ileum tissue. Conclusion: Supplementation of QUE and SB demonstrated the ability to relieve oxidative stress in pullets post DIQ-injection with a time-dependent manner and QUE and SB may be potential antioxidant additives for relieving oxidative stress and protecting the intestinal barrier of pullets.
참고문헌 (30)
- Frankič T, "The comparison of in vivo antigenotoxic and antioxidative capacity of two propylene glycol extracts of Calendula officinalis (marigold) and vitamin E in young growing pigs", J Anim Physiol Anim Nutr, 93, pp.688~694 (2009)[DOI]
- Celi P, "Effects of plane of nutrition on oxidative stress in goats during the peripartum period", Vet J, 184, pp.95~99 (2010)[DOI]
- Mishra B, "Oxidative stress in the poultry gut : potential challenges and interventions", Front Vet Sci, 6, pp.60~ (2019)[DOI]
- Wang Y, "Bacillus amyloliquefaciens SC06 alleviates the oxidative stress of IPEC-1 via modulating Nrf2", Keap1 signaling pathway and decreasing ROS production, 2017, pp.101, 3015~3026
- Li YH, "Dietary iron deficiency and oversupplementation increase intestinal permeability, ion transport, and inflammation in pigs", J Nutr, 146, pp.1499~1505 (2016)[DOI]
- Mitic LL, "Molecular physiology and pathophysiology of tight junctions I. Tight junction structure and function: lessons from mutant animals and proteins", Am J Physiol Gastrointest Liver Physiol, 279, pp.G250~G254 (2000)[DOI]
- Cao ST, "Diquat-induced oxidative stress increases intestinal permeability, impairs mitochondrial function, and triggers mitophagy in piglets", J Anim Sci, 96, pp.1795~1805 (2018)[DOI]
- Li M, "Dietary puerarin supplementation alleviates oxidative stress in the small intestines of diquat-challenged piglets", Animals(Basel), 10, pp.631~ (2020)[DOI]
- Chen YN, "Pterostilbene as a protective antioxidant attenuates diquat-induced liver injury and oxidative stress in 21-day-old broiler chickens", Poult Sci, 99, pp.3158~3167 (2020)[DOI]
- Yuan DX, "The evaluation of antioxidant and anti-inflammatory effects of eucommia ulmoides flavones using diquat-challenged piglet models", Oxid Med Cell Longev, 2017, pp.8140962~ (2017)[DOI]
- Wang SG, "Bacteriostatic effect of quercetin as an antibiotic alternative in vivo and its antibacterial mechanism in vitro", J Food Prot, 81, pp.68~78 (2018)[DOI]
- Luo MJ, "Quercetin suppressed NADPH oxidase-derived oxidative stress via heme oxygenase-1 induction in macrophages", Arch Biochem Biophys, 671, pp.69~76 (2019)[DOI]
- Dong YY, "Effects of dietary quercetin on the antioxidative status and cecal microbiota in broiler chickens fed with oxidized oil", Poult Sci, 99, pp.4892~4903 (2020)[DOI]
- Sun L, "Quercetin protects against lipopolysaccharide-induced intestinal oxidative stress in broiler chickens through activation of Nrf2 pathway", Molecules, 25, pp.1053~ (2020)[DOI]
- Chang PV, "The microbial metabolite butyrate regulates intestinal macrophage function via histone deacetylase inhibition", Proc Natl Acad Sci USA, 111, pp.2247~2252 (2014)[DOI]
- Kanai T, "A breakthrough in probiotics : Clostridium butyricum regulates gut homeostasis and antiinflammatory response in inflammatory bowel disease", J Gastroenterol, 50, pp.928~939 (2015)[DOI]
- Song BC, "Effect of microencapsulated sodium butyrate dietary supplementation on growth performance and intestinal barrier function of broiler chickens infected with necrotic enteritis", Anim Feed Sci Technol, 232, pp.6~15 (2017)[DOI]
- Zhang WH, "Dietary sodium butyrate alleviates the oxidative stress induced by corticosterone exposure and improves meat quality in broiler chickens", Poult Sci, 90, pp.2592~2599 (2011)[DOI]
- Zou X, "Effects of sodium butyrate on intestinal health and gut microbiota composition during intestinal inflammation progression in broilers", Poult Sci, 98, pp.4449~4456 (2019)[DOI]
- Wen CY, "Taurine alleviates intestinal injury by mediating tight junction barriers in diquat-challenged piglet models", Front Physiol, 11, pp.449~ (2020)[DOI]
- Su Jeong Kim, "Quantification of Quercetin in Different Parts of Onion and Its DPPHRadical Scavenging and Antibacterial Activity", Food Science and Biotechnology, 15(1), pp.39~43 (2006), 한국식품과학회
- Zou Y, "Protective effect of quercetin on pig intestinal integrity after transport stress is associated with regulation oxidative status and inflammation", J Vet Med Sci, 78, pp.1487~1494 (2016)[DOI]
- Liu WH, "Supplementation with sodium butyrate improves growth and antioxidant function in dairy calves before weaning", J Anim Sci Biotechnol, 12, pp.2~ (2021)[DOI]
- Hao LH, "Pediococcus pentosaceus ZJUAF-4 relieves oxidative stress and restores the gut microbiota in diquat-induced intestinal injury", Appl Microbiol Biotechnol, 105, pp.1657~1668 (2021)[DOI]
- Suzuki T, "Toward clinical application of the Keap1-Nrf2 pathway", Trends Pharmacol Sci, 34, pp.340~346 (2013)[DOI]
- Liu L, "Selenium-enriched yeast alleviates oxidative stress-induced intestinal mucosa disruption in weaned pigs", Oxid Med Cell Longev, 2020, pp.5490743~ (2020)[DOI]
- Entaz B, "Quercetin attenuates manganeseinduced neuroinflammation by alleviating oxidative stress through regulation of apoptosis, iNOS", NF-κB and HO-1, Int J Mol Sci, pp.2017, 18[DOI]
- Ma N, "Sodium butyrate improves antioxidant stability in sub-acute ruminal acidosis in dairy goats", BMC Vet Res, 14, pp.275~ (2018)[DOI]
- Anderson JM, "Tight junctions and the molecular basis for regulation of paracellular permeability", Am J Physiol Gastrointest Liver Physiol, 269, pp.G467~G475 (1995)[DOI]
- Zhang K, "Sodium butyrate improves high-concentrate-diet-induced impairment of ruminal epithelium barrier function in goats", J Agric Food Chem, 66, pp.8729~8736 (2018)[DOI]
