Fermentative products and bacterial community structure of C4 forage silage in response to epiphytic microbiota from C3 forages
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Objective: The observation that temperate C3 and tropical C4 forage silages easily produce large amounts of ethanol or acetic acid has puzzled researchers for many years. Hence, this study aimed to assess the effects of epiphytic microbiota from C3 forages (Italian ryegrass and oat) on fermentative products and bacterial community structure in C4 forage (sorghum) silage. Methods: Through microbiota transplantation and γ-ray irradiation sterilization, the irradiated sorghum was treated: i) sterile distilled water (STSG); ii) epiphytic microbiota from sorghum (SGSG); iii) epiphytic microbiota from Italian ryegrass (SGIR); iv) epiphytic microbiota from oat (SGOT). Results: After 60 days, all the treated groups had high lactic acid (>63.0 g/kg dry matter [DM]) contents and low pH values (<3.70), acetic acid (<14.0 g/kg DM) and ammonia nitrogen (<80.0 g/kg total nitrogen) contents. Notably, SGIR (59.8 g/kg DM) and SGOT (77.6 g/kg DM) had significantly (p<0.05) higher ethanol concentrations than SGSG (14.2 g/kg DM) on day 60. After 60 days, Lactobacillus were predominant genus in three treated groups. Higher proportions of Chishuiella (12.9%) and Chryseobacterium (7.33%) were first found in silages. The ethanol contents had a positive correlation (p<0.05) with the abundances of Chishuiella, Acinetobacter, Stenotrophomonas, Chryseobacterium, and Sphingobacterium. Conclusion: The epiphytic bacteria on raw materials played important roles in influencing the silage fermentation products between temperate C3 and tropical C4 forages. The quantity and activity of hetero-fermentative Lactobacillus, Chishuiella, Acinetobacter, Stenotrophomonas, Chryseobacterium, and Sphingobacterium may be the key factors for the higher ethanol contents and DM loss in silages.
Abstract
Objective: The observation that temperate C3 and tropical C4 forage silages easily produce large amounts of ethanol or acetic acid has puzzled researchers for many years. Hence, this study aimed to assess the effects of epiphytic microbiota from C3 forages (Italian ryegrass and oat) on fermentative products and bacterial community structure in C4 forage (sorghum) silage. Methods: Through microbiota transplantation and γ-ray irradiation sterilization, the irradiated sorghum was treated: i) sterile distilled water (STSG); ii) epiphytic microbiota from sorghum (SGSG); iii) epiphytic microbiota from Italian ryegrass (SGIR); iv) epiphytic microbiota from oat (SGOT). Results: After 60 days, all the treated groups had high lactic acid (>63.0 g/kg dry matter [DM]) contents and low pH values (<3.70), acetic acid (<14.0 g/kg DM) and ammonia nitrogen (<80.0 g/kg total nitrogen) contents. Notably, SGIR (59.8 g/kg DM) and SGOT (77.6 g/kg DM) had significantly (p<0.05) higher ethanol concentrations than SGSG (14.2 g/kg DM) on day 60. After 60 days, Lactobacillus were predominant genus in three treated groups. Higher proportions of Chishuiella (12.9%) and Chryseobacterium (7.33%) were first found in silages. The ethanol contents had a positive correlation (p<0.05) with the abundances of Chishuiella, Acinetobacter, Stenotrophomonas, Chryseobacterium, and Sphingobacterium. Conclusion: The epiphytic bacteria on raw materials played important roles in influencing the silage fermentation products between temperate C3 and tropical C4 forages. The quantity and activity of hetero-fermentative Lactobacillus, Chishuiella, Acinetobacter, Stenotrophomonas, Chryseobacterium, and Sphingobacterium may be the key factors for the higher ethanol contents and DM loss in silages.
참고문헌 (35)
- McDonald P, "The Biochemistry of silage" (1991), Chalcombe Publications
- Nayyar H, "Differential sensitivity of C3 and C4plants to water deficit stress : association with oxidative stress and antioxidants", Environ Exp Bot, 58, pp.106~113 (2006)[DOI]
- Ward JK, "Comparative responses of model C3 and C4 plants to drought in low and elevated CO2", Global Change Biol, 5, pp.857~867 (1999)[DOI]
- Li Y, "Changes in the bacterial community and composition of fermentation products during ensiling of wilted Italian ryegrass and wilted guinea grass silages", Anim Sci J, 84, pp.607~612 (2013)[DOI]
- Driehuis F, "The occurrence and prevention of ethanol fermentation in high-dry-matter grass silage", J Sci Food Agric, 80, pp.711~718 (2000)[DOI]
- Tao Shao, "Comparison of Fermentation Characteristics of Italian Ryegrass (Lolium multiflorum Lam.) and Guineagrass (Panicum maximum Jacq.) during the Early Stage of Ensiling", Animal Bioscience, 18(12), pp.1727~1734 (2005), 아세아·태평양축산학회
- Buxton DR, "Silage science and technology", pp.c5~ (2003), ASA, CSSA, SSSA Books
- Duniere L, "Bacterial and fungal core microbiomes associated with small grain silages during ensiling and aerobic spoilage", BMC Microbiol, 17, pp.50~ (2017)[DOI]
- Nazar M, "Abundance and diversity of epiphytic microbiota on forage crops and their fermentation characteristic during the ensiling of sterile sudan grass", World J Microbiol Biotechnol, 37, pp.27~ (2021)[DOI]
- Wang S, "Exploring the ensiling characteristics and bacterial community of red clover inoculated with the epiphytic bacteria from temperate gramineous grasses", J Appl Microbiol, 132, pp.177~188 (2022)[DOI]
- Miron J, "Field yield, ensiling properties and digestibility by sheep of silages from two forage sorghum varieties", Anim Feed Sci Technol, 136, pp.203~215 (2007)[DOI]
- Mogodiniyai Kasmaei K, "Separating the effects of forage source and field microbiota on silage fermentation quality and aerobic stability", Grass Forage Sci, 72, pp.281~289 (2016)[DOI]
- Junges D, "Influence of various proteolytic sources during fermentation of reconstituted corn grain silages", J Dairy Sci, 100, pp.9048~9051 (2017)[DOI]
- Playne MJ, "The buffering constituents of herbage and of silage", J Sci Food Agric, 17, pp.264~268 (1966)[DOI]
- Broderick GA, "Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media", J Dairy Sci, 63, pp.64~75 (1980)[DOI]
- Krishnamoorthy U, "Nitrogen fractions in selected feedstuffs", J Dairy Sci, 65, pp.217~225 (1982)[DOI]
- Thomas TA, "An automated procedure for the determination of soluble carbohydrates in herbage", J Sci Food Agric, 28, pp.639~642 (1977)[DOI]
- Van Soest PJ, "Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition", J Dairy Sci, 74, pp.3583~3597 (1991)[DOI]
- Wang S, "Sequencing and microbiota transplantation to determine the role of microbiota on the fermentation type of oat silage", Bioresour Technol, 309, pp.123371~ (2020)[DOI]
- Liu B, "Dynamics of a microbial community during ensiling and upon aerobic exposure in lactic acid bacteria inoculation-treated and untreated barley silages", Bioresour Technol, 273, pp.212~219 (2019)[DOI]
- Du Z, "Microbial community structure, co-occurrence network and fermentation characteristics of woody plant silage", J Sci Food Agric, 102, pp.1193~1204 (2022)[DOI]
- Ma SS, "Bacterial community succession during pig manure and wheat straw aerobic composting covered with a semi-permeable membrane under slight positive pressure", Bioresour Technol, 259, pp.221~227 (2018)[DOI]
- Wang S, "Assessment of inoculating various epiphytic microbiota on fermentative profile and microbial community dynamics in sterile Italian ryegrass", J Appl Microbiol, 129, pp.509~520 (2020)[DOI]
- Keshri J, "Microbiome dynamics during ensiling of corn with and without Lactobacillus plantarum inoculant", Appl Microbiol Biotechnol, 102, pp.4025~4037 (2018)[DOI]
- Graf K, "Bacterial community dynamics during ensiling of perennial ryegrass at two compaction levels monitored by terminal restriction fragment length polymorphism", J Appl Microbiol, 120, pp.1479~1491 (2016)[DOI]
- Borreani G, "Silage review : Factors affecting dry matter and quality losses in silages", J Dairy Sci, 101, pp.3952~3979 (2018)[DOI]
- Duniere L, "Silage processing and strategies to prevent persistence of undesirable microorganisms", Anim Feed Sci Technol, 182, pp.1~15 (2013)[DOI]
- Nishino N, "Effects of wilting and molasses addition on fermentation and bacterial community in guinea grass silage", Lett Appl Microbiol, 54, pp.175~181 (2012)[DOI]
- Liu QH, "Determination of aerobic deterioration of corn stalk silage caused by aerobic bacteria", Anim Feed Sci Technol, 183, pp.124~131 (2013)[DOI]
- Zhang RG, "Description of Chishuiella changwenlii gen. nov., sp. nov., isolated from freshwater, and transfer of Wautersiella falsenii to the genus Empedobacter as Empedobacter falsenii comb. nov", Int J Syst Evol Microbiol, 64, pp.2723~2728 (2014)[DOI]
- "Chryseobacterium. Bergey’s Manual of Systematics of Archaea and Bacteria" (2019)[URL]
- Kung Jr L, "Silage review : Interpretation of chemical, microbial, and organoleptic components of silages", J Dairy Sci, 101, pp.4020~4033 (2018)[DOI]
- Pahlow G, "Silage science and technology", pp.31~93 (2003), ASA, CSSA, and SSSA
- McDonald P, "Animal nutrition", pp.693~ (2002), Pearson Education Limited
- Shankar K, "Saccharification of the pretreated husks of corn, peanut and coffee cherry by the lignocellulolytic enzymes secreted by Sphingobacterium sp. ksn for the production of bioethanol", Biomass Bioenergy, 127, pp.105298~ (2019)[DOI]