본문 바로가기
KERD
논문KCI 등재

Effect of knife castration on leukocyte cytokine expression and indicators of stress, pain, and inflammation in Korean cattle bull calves

0

조회

0

다운로드

1

인용

0

참고문헌

키워드

초록

Objective: This study investigated the effects of surgical castration on behavior, physiological and inflammatory indicators, and leukocyte cytokine mRNA levels in Korean cattle bull calves. Methods: Nineteen Korean cattle bull calves (average body weight, 254.5 kg; average age, 8.2 months) were divided into two treatment groups: control (n = 9) and castration (n = 10). Surgical castration was performed using Newberry knives and a Henderson castrating tool. Blood was obtained just before castration (0 h) and at 0.5 h, 6 h, 1 d, 3 d, 7 d, and 14 d after castration. Plasma cortisol (PC), saliva cortisol (SC), plasma substance P, and plasma haptoglobin concentrations, and the leucocyte mRNA levels of the interleukin-1-alpha (IL1A), interleukin-1-beta (IL1B), interleukin-1 receptor antagonist (IL1RN), and interleukin-6 (IL6) genes were analyzed. Results: Castration decreased (p<0.01) the average daily gain and gain/feed ratio. Castration reduced the time spent eating (p<0.001) and the eating frequency (p<0.01) and increased (p<0.001) the lying frequency. Castration temporarily increased (p<0.05) circulating PC and SC concentrations at 0.5 h after castration. Castration temporarily increased (p<0.05) plasma substance P concentrations at 1 d after castration. Castration increased (p<0.05) plasma haptoglobin concentrations at 1 and 3 d after castration. Castration increased (p< 0.05) leukocyte mRNA levels of the IL1A, IL1B, IL1RN, and IL6 genes at 6 h after castration. Conclusion: Castration temporarily induced stress and expression of leucocyte inflammatory cytokine genes in Korean cattle bull calves.

Abstract

Objective: This study investigated the effects of surgical castration on behavior, physiological and inflammatory indicators, and leukocyte cytokine mRNA levels in Korean cattle bull calves. Methods: Nineteen Korean cattle bull calves (average body weight, 254.5 kg; average age, 8.2 months) were divided into two treatment groups: control (n = 9) and castration (n = 10). Surgical castration was performed using Newberry knives and a Henderson castrating tool. Blood was obtained just before castration (0 h) and at 0.5 h, 6 h, 1 d, 3 d, 7 d, and 14 d after castration. Plasma cortisol (PC), saliva cortisol (SC), plasma substance P, and plasma haptoglobin concentrations, and the leucocyte mRNA levels of the interleukin-1-alpha (IL1A), interleukin-1-beta (IL1B), interleukin-1 receptor antagonist (IL1RN), and interleukin-6 (IL6) genes were analyzed. Results: Castration decreased (p<0.01) the average daily gain and gain/feed ratio. Castration reduced the time spent eating (p<0.001) and the eating frequency (p<0.01) and increased (p<0.001) the lying frequency. Castration temporarily increased (p<0.05) circulating PC and SC concentrations at 0.5 h after castration. Castration temporarily increased (p<0.05) plasma substance P concentrations at 1 d after castration. Castration increased (p<0.05) plasma haptoglobin concentrations at 1 and 3 d after castration. Castration increased (p< 0.05) leukocyte mRNA levels of the IL1A, IL1B, IL1RN, and IL6 genes at 6 h after castration. Conclusion: Castration temporarily induced stress and expression of leucocyte inflammatory cytokine genes in Korean cattle bull calves.

참고문헌 (30)

  1. "Welfare implications of castration of cattle" (2014)[URL]
  2. 박승주, "Genetic, management, and nutritional factors affecting intramuscular fat deposition in beef cattle — A review", Animal Bioscience, 31(7), pp.1043~1061 (2018), 아세아·태평양축산학회[DOI]
  3. Park SJ, "Effects of castration and a lidocaine-plus-flunixin treatment on growth and indicators of pain, inflammation, and liver function in Korean cattle bull calves", Livest Sci, 216, pp.61~66 (2018)[DOI]
  4. Sutherland MA, "Effect of castration and dehorning singularly or combined on the behavior and physiology of Holstein calves", J Anim Sci, 91, pp.935~942 (2013)[DOI]
  5. González LA, "Pain mitigation after band castration of beef calves and its effects on performance, behavior, Escherichia coli, and salivary cortisol", J Anim Sci, 88, pp.802~810 (2010)[DOI]
  6. Earley B, "Effects of ketoprofen alone or in combination with local anesthesia during the castration of bull calves on plasma cortisol, immunological, and inflammatory responses", J Anim Sci, 80, pp.1044~1052 (2002)[DOI]
  7. Warnock TM, "Effect of castration technique on beef calf performance, feed efficiency, and inflammatory response", J Anim Sci, 90, pp.2345~2352 (2012)[DOI]
  8. Beutler B, "Cachectin", tumor necrosis factor : an endogenous mediator of shock and inflammation, 1986, pp.5, 281~293
  9. Horwitz W, "Official methods of analysis of AOAC International" (2005), AOAC International
  10. Van Soest PV, "Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition", J Dairy Sci, 74, pp.3583~3597 (1991)[DOI]
  11. Hernandez CE, "Time lag between peak concentrations of plasma and salivary cortisol following a stressful procedure in dairy cattle", Acta Vet Scand, 56, pp.61~ (2014)[DOI]
  12. O'Loughlin A, "Examination of the bovine leukocyte environment using immunogenetic biomarkers to assess immunocompetence following exposure to weaning stress", BMC Vet Res, 7, pp.45~ (2011)[DOI]
  13. Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method", methods, 25, pp.402~408 (2001)[DOI]
  14. McKay LI, "Holland-Frei cancer medicine" (2003), BC Decker
  15. Meléndez DM, "Effect of band and knife castration of beef calves on welfare indicators of pain at three relevant industry ages : I. Acute pain", J Anim Sci, 95, pp.4352~4366 (2017)[DOI]
  16. Schwinn AC, "Suitability of saliva cortisol as a biomarker for hypothalamic–pituitary–adrenal axis activation assessment, effects of feeding actions, and immunostimulatory challenges in dairy cows", J Anim Sci, 94, pp.2357~2365 (2016)[DOI]
  17. Vining RF, "The measurement of hormones in saliva : possibilities and pitfalls", J Steroid Biochem, 27, pp.81~94 (1987)[DOI]
  18. Coetzee JF, "Plasma concentrations of substance P and cortisol in beef calves after castration or simulated castration", Am J Vet Res, 69, pp.751~762 (2008)[DOI]
  19. Mintline EM, "Healing of surgical castration wounds : a description and an evaluation of flunixin", J Anim Sci, 92, pp.5659~5665 (2014)[DOI]
  20. Murata H, "Current research on acute phase proteins in veterinary diagnosis : an overview", Vet J, 168, pp.28~40 (2004)[DOI]
  21. Jain S, "Acute-phase proteins : as diagnostic tool", J Pharm Bioallied Sci, 3, pp.118~ (2011)[DOI]
  22. Horadagoda NU, "Acute phase proteins in cattle : discrimination between acute and chronic inflammation", Vet Rec, 144, pp.437~441 (1999)[DOI]
  23. Brown AC, "Effect of castration timing and oral meloxicam administration on growth performance, inflammation, behavior, and carcass quality of beef calves", J Anim Sci, 93, pp.2460~2470 (2015)[DOI]
  24. Di Paolo NC, "Interleukin 1α and the inflammatory process", Nat Immunol, 17, pp.906~913 (2016)[DOI]
  25. Pang WY, "Banding or Burdizzo castration and carprofen administration on peripheral leukocyte inflammatory cytokine transcripts", Res Vet Sci, 90, pp.127~132 (2011)[DOI]
  26. Schindler R, "Correlations and interactions in the production of interleukin-6(IL-6), IL-1, and tumor necrosis factor(TNF)in human blood mononuclear cells : IL-6 suppresses IL-1and TNF", Bood, 75, pp.40~47 (1990)[DOI]
  27. Arend WP, "Physiologic role of interleukin-1 receptor antagonist", Arthritis Res Ther, 2, pp.245~ (2000)[DOI]
  28. Arend WP, "Interleukin-1receptor antagonist : role in biology", Annu Rev Immunol, 16, pp.27~55 (1998)[DOI]
  29. Steensberg A, "IL-6 enhances plasma IL-1ra, IL-10, and cortisol in humans", Am J Physiol Endocrinol Metab, 285, pp.E433~ (2003)[DOI]
  30. Nicklin MJ, "Arterial inflammation in mice lacking the interleukin 1 receptor antagonist gene", J Exp Med, 191, pp.303~312 (2000)[DOI]

관련 논문AI추천

Effect of surgical castration treatments on blood parameters and behavioral characteristics in Korean native cattle (Hanwoo)Byeong-Soo Kim한국동물위생학회 · 한국동물위생학회지2016 · 제39권 제4호한우에서 거세에 의한 Testosterone 결핍이 사이토카인의 변화에 미치는 영향 연구김성범, 김은주, 도윤정, 유재규, 정영훈, 조아라, 최창용한국동물위생학회 · 한국동물위생학회지2017 · 제40권 제4호Effect of short-term fattening period and castration method on productivity, serum testosterone, and economic efficacy in Hanwoo cattle박병기, 안준상, 원정일, 이현정, 장선식, Eung Gi Kwon, Ui-Hyung Kim한국축산학회 · 한국축산학회지2023 · 제65권 제1호Effects of weaning and castration ages on growth performance, blood metabolites, and carcass characteristics in Hanwoo steersByung Ki Park, Eung Gi Kwon1, Gi Hwal Son, Hwan Lim, Il Young Kim, Jae Yoon Shim, Ji Hyung Kim, Jong Suh Shin, Joong Kook Park, Jun Sang Ahn, Min Ji Kim, Sung Myoun Cho한국축산학회 · 한국축산학회지2018 · 제60권 제12호Welfare traits of Bos indicus cattle castrated immunologically and fed beta-adrenergic agonistsAntonelo Daniel Silva, Consolo Nara Regina Brandao, Leme Paulo Roberto, Martello Luciane Silva, Negrão João Alberto, Pacheco Verônica Madeira, Rosa Alessandra Fernandes, Silva Saulo da Luz, Sousa Rafael Vieira아세아·태평양축산학회 · Animal Bioscience2021 · 제34권 제9호사육면적의 차이가 한우의 사양성적, 행동특성 및 혈액성상에 미치는 영향김병기, 송영한, 양가영, 오동엽, 이준구, 하재정동물생명과학연구소 · 동물자원연구2015 · 제26권 제2호

이 간행물의 다른 논문 (12건)