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12-Oxoeicosatetraenoic acid, a candidate signal for placenta separation, activates matrix metalloproteinase and induces apoptosis in bovine trophoblast cells

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Objective: 12-oxo-5Z,8Z,10E,14Z-eicosatetraenoic acid (12-KETE), a metabolite of arachidonic acid, is a strong candidate signal for placenta separation following calf discharge at delivery. In the present study, the effects of 12-KETE on bovine trophoblast cells were investigated to determine its function in the placentome at delivery. Methods: Bovine trophoblast cells derived from blastocysts were used. They were cocultured with or without fibroblasts derived from bovine placentome and/or bovine uterine epithelial cells. 12-KETE was added to the culture medium. Results: Bovine trophoblast cells contained binucleate cells and strongly expressed caudal type homeobox 2 (CDX-2) genes. Addition of 12-KETE to the trophoblast cell colony without feeder cells or that on a fibroblast monolayer induced rapid exfoliation of the colony. After 12-KETE addition, trophoblast cells emitted strong fluorescence caused by the degradation of dye-quenched collagen, indicating that 12-KETE activated matrix metalloproteinase of the trophoblast cells. Exfoliated cell colonies were stained with YOPRO-1, but not propidium iodide (PI). Moreover, DNA fragmentation and Bcl-2 associated X protein (Bax) gene (apoptosis stimulator) upregulation were observed in exfoliated cells, indicating that 12- KETE induced trophoblast cell apoptosis. These results were consistent with previous in vivo observations; however, even a lower concentration of 12-KETE activated trophoblast protease. Meanwhile, fibroblasts derived from the bovine placentome converted arachidonic acid to 12-KETE. Conclusion: These observations indicate that 12-KETE may serve as a signal for placenta separation at delivery.

Abstract

Objective: 12-oxo-5Z,8Z,10E,14Z-eicosatetraenoic acid (12-KETE), a metabolite of arachidonic acid, is a strong candidate signal for placenta separation following calf discharge at delivery. In the present study, the effects of 12-KETE on bovine trophoblast cells were investigated to determine its function in the placentome at delivery. Methods: Bovine trophoblast cells derived from blastocysts were used. They were cocultured with or without fibroblasts derived from bovine placentome and/or bovine uterine epithelial cells. 12-KETE was added to the culture medium. Results: Bovine trophoblast cells contained binucleate cells and strongly expressed caudal type homeobox 2 (CDX-2) genes. Addition of 12-KETE to the trophoblast cell colony without feeder cells or that on a fibroblast monolayer induced rapid exfoliation of the colony. After 12-KETE addition, trophoblast cells emitted strong fluorescence caused by the degradation of dye-quenched collagen, indicating that 12-KETE activated matrix metalloproteinase of the trophoblast cells. Exfoliated cell colonies were stained with YOPRO-1, but not propidium iodide (PI). Moreover, DNA fragmentation and Bcl-2 associated X protein (Bax) gene (apoptosis stimulator) upregulation were observed in exfoliated cells, indicating that 12- KETE induced trophoblast cell apoptosis. These results were consistent with previous in vivo observations; however, even a lower concentration of 12-KETE activated trophoblast protease. Meanwhile, fibroblasts derived from the bovine placentome converted arachidonic acid to 12-KETE. Conclusion: These observations indicate that 12-KETE may serve as a signal for placenta separation at delivery.

참고문헌 (29)

  1. Beagley JC, "Physiology and treatment of retained fetal membranes in cattle", J Vet Intern Med, 24, pp.261~268 (2010)[DOI]
  2. Garcia A, "The effect of treatment with cloprostenol or dinoprost within one hour of induced parturition on the incidence of retained placenta in cattle", Can Vet J, 33, pp.175~183 (1992)
  3. Stevens RD, "Treatment of dairy cow at parturition with PGF2alpha or oxytocin for prevention of retained fetal membranes", J Am Vet Med Assoc, 211, pp.1280~1284 (1997)
  4. Nakao T, "Postpartum plasma PGF metabolite profile in cows with dystocia and", or retained placenta, and effect of fenprostalene on uterine involution and reproductive performance, 1997, pp.59, 791~794
  5. Maj JG, "Activity of 72-kDa and 92-kDa matrix metalloproteinases in placental tissues of cows with and without retained fetal membranes", Placenta, 18, pp.683~687 (1997)[DOI]
  6. Dilly M, "Expression of matrix metalloproteinase(MMP)-2, MMP-14 and tissue inhibitor of matrix metalloproteinase(TIMP)-2 during bovine placentation and at term with or without placental retention", Theriogenology, 75, pp.1104~1114 (2011)[DOI]
  7. Hirayama H, "Differences in apoptotic status in the bovine placentome between spontaneous and induced parturition", J Reprod Dev, 58, pp.585~591 (2012)[DOI]
  8. Kamemori Y, "Expression of apoptosis-regulating factors in bovine retained placenta", Placenta, 32, pp.20~26 (2011)[DOI]
  9. Kamada H, "Twelve oxo-eicosatetraenoic acid induces fetal membrane release after delivery in cows", Placenta, 33, pp.106~113 (2012)[DOI]
  10. Wright JM, "Manual of the International Embryo Transfer Society: a procedural guide and general information for the use of embryo transfer technology emphasizing sanitary procedure", pp.141~144 (2010), International Embryo Transfer Society
  11. Takahashi H, "Isolation and culture of bovine endometrial epithelial cells in a serumfree culture system", J Reprod Dev, 47, pp.181~187 (2001)[DOI]
  12. Jang HY, "Antioxidative Effects of Astaxanthin against Nitric Oxide-Induced Oxidative Stress on Cell Viability and Gene Expression in Bovine Oviduct Epithelial Cell and the Developmental Competence of Bovine IVM", IVF Embryos, 2010, pp.45, 967~974
  13. Zhao S, "A three-dimensional culture system using alginate hydrogel prolongs hatched cattle embryo development in vitro", Theriogenology, 84, pp.184~192 (2015)[DOI]
  14. Ghajar CM, "Mesenchymal cells stimulate capillary morphogenesis via distinct proteolytic mechanisms", Exp Cell Res, 316, pp.813~825 (2010)[DOI]
  15. Hadler-Olsen E, "Gelatin in situ zymography on fixed, paraffin-embedded tissue : zinc and ethanol fixation preserve enzyme activity", J Histochem Cytochem, 58, pp.29~39 (2010)[DOI]
  16. Fujisawa S, "Evaluation of YO-PRO-1as an early marker of apoptosis following radiofrequency ablation of colon cancer liver metastases", Cytotechnology, 66, pp.259~273 (2014)[DOI]
  17. Jedrusik A, "Maternal-zygotic knockout reveals a critical role of Cdx2in the morula to blastocyst transition", Dev Biol, 398, pp.147~152 (2015)[DOI]
  18. Eiler H, "Successful treatment of retained placenta with umbilical cord injections of collagenase in cows", J Am Vet Med Assoc, 203, pp.436~443 (1993)
  19. Guilbault LA, "Relationship of 15-keto-13, 14-dihydro-prostaglandin F2alpha concentrations in peripheral plasma with local uterine production of F series prostaglandins and changes in uterine blood flow during the early postpartum period of cattle", Biol Reprod, 31, pp.870~878 (1984)[DOI]
  20. Peter AT, "Peripartal endocrine changes associated with retained placenta in dairy cows", Theriogenology, 28, pp.383~394 (1987)[DOI]
  21. Godkin JD, "Phospholipase A2 regulation of bovine endometrial(BEND)cell prostaglandin production", Reprod Biol Endocrinol, 6, pp.44~ (2008)[DOI]
  22. Walstra P, "Demonstration of a 12-lipoxygenase activity in bovine polymorphonuclear leukocytes", Biochim Biophys Acta, 921, pp.312~319 (1987)[DOI]
  23. Tanihiro Yoshimoto, "Arachidonate 12-lipoxygenases", Prostaglandins & Other Lipid Mediators, 68-69, pp.245~262 (2002), Elsevier BV[DOI]
  24. Gunnink JW, "Retained placenta and leucocytic activity", Vet Q, 6, pp.49~51 (1984)[DOI]
  25. Heuwieser W, "Significance of chemotactic activity for placental expulsion in cattle", Theriogenology, 27, pp.907~912 (1987)[DOI]
  26. Benedictus L, "Chemotactic activity of cotyledons for mononuclear leukocytes related to occurrence of retained placenta in dexamethasone induced parturition in cattle", Theriogenology, 76, pp.802~809 (2011)[DOI]
  27. Schrey MP, "A role for phosphoinositide hydrolysis in human uterine smooth muscle during parturition", Am J Obstet Gynecol, 159, pp.964~970 (1988)[DOI]
  28. Silvia WJ, "Role of phospholipase C in mediating oxytocin-induced release of prostaglandin F2α from ovine endometrial tissue", Prostaglandins, 35, pp.535~548 (1988)[DOI]
  29. Shenavai S, "Investigations into the mechanisms controlling parturition in cattle", Reproduction, 144, pp.279~292 (2012)[DOI]

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