Expression of heat shock protein genes in Simmental cattle exposed to heat stress
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objective: In tropical, subtropical and arid zones, heat stress is the main cause of productivity reduction in cattle. When climate stressors occur, animals become thermal adapted through differential expression of some genes, including heat shock proteins (HSP) family. The aim of this study was to determine levels of expression of HSP60, HSP70, and HSP90 genes in Simmental cattle raised in tropical environments of Mexico. Methods: In this study, expression of HSP60, HSP70, and HSP90 genes was analyzed in 116 Simmental cattle from three farms with tropical climate located in western Mexico. Animals were sampled twice a day, in the morning and noon. Gene expression was evaluated by quantitative polymerase chain reaction using probes marked with fluorescence. The MIXED procedure of SAS with repeated measures was used for all statistical analysis. Results: HSP60 gene expression differences were found for sex (p = 0.0349). HSP70 gene differences were detected for sampling hour (p = 0.0042), farm (p<0.0001), sex (p = 0.0476), and the interaction sampling hour×farm (p = 0.0002). Gene expression differences for HSP90 were observed for farm (p<0.0001) and year (p = 0.0521). HSP70 gene showed to be a better marker of heat stress than HSP60 and HSP90 genes. Conclusion: Expression of HSP70 gene in Simmental herds of the tropical region of western México was different during early morning and noon, but the expression of the HSP60 and HSP90 genes was similar. Identification of resilient animals to heat stress will be useful in the genetic improvement of the Simmental breed.
Abstract
Objective: In tropical, subtropical and arid zones, heat stress is the main cause of productivity reduction in cattle. When climate stressors occur, animals become thermal adapted through differential expression of some genes, including heat shock proteins (HSP) family. The aim of this study was to determine levels of expression of HSP60, HSP70, and HSP90 genes in Simmental cattle raised in tropical environments of Mexico. Methods: In this study, expression of HSP60, HSP70, and HSP90 genes was analyzed in 116 Simmental cattle from three farms with tropical climate located in western Mexico. Animals were sampled twice a day, in the morning and noon. Gene expression was evaluated by quantitative polymerase chain reaction using probes marked with fluorescence. The MIXED procedure of SAS with repeated measures was used for all statistical analysis. Results: HSP60 gene expression differences were found for sex (p = 0.0349). HSP70 gene differences were detected for sampling hour (p = 0.0042), farm (p<0.0001), sex (p = 0.0476), and the interaction sampling hour×farm (p = 0.0002). Gene expression differences for HSP90 were observed for farm (p<0.0001) and year (p = 0.0521). HSP70 gene showed to be a better marker of heat stress than HSP60 and HSP90 genes. Conclusion: Expression of HSP70 gene in Simmental herds of the tropical region of western México was different during early morning and noon, but the expression of the HSP60 and HSP90 genes was similar. Identification of resilient animals to heat stress will be useful in the genetic improvement of the Simmental breed.
참고문헌 (22)
- Hristov AN, "Climate change effects on livestock in the Northeast US and strategies for adaptation", Clim Change, 146, pp.33~45 (2018)[DOI]
- Sejian V, "Climate change, impact on production and reproduction, adaptation mechanisms and mitigation strategies in small ruminants, a review", Indian J Small Rumin, 19, pp.1~21 (2013)
- Hall LW, "climatology: new developments", pp.43~62 (2012), Nova Science Publishers, Inc
- Baena MM, "Heat tolerance responses in a Bos taurus cattle herd raised in a Brazilian climate", J Therm Biol, 81, pp.162~169 (2019)[DOI]
- Bretanha R, "Differential expression of HSF1 and HSPA6 genes and physiological responses in Angus and Simmental cattle breeds", J Therm Biol, 84, pp.92~98 (2019)[DOI]
- Cunningham E, "Animal production and health", pp.68~ (1987), FAO
- Beckham JT, "Assessment of cellular response to thermal laser injury through bioluminescence imaging of heat shock protein 70", J Photochem Photobiol, 79, pp.76~85 (2004)[DOI]
- Bharati J, "Expression dynamics of HSP90 and nitric oxide synthase(NOS)isoforms during heat stress acclimation in Tharparkar cattle", Int J Biometeorol, 61, pp.1461~1469 (2017)[DOI]
- Kumar J, "Impact of season on antioxidants, nutritional metabolic status, cortisol and heat shock proteins in Hariana and Sahiwal cattle", Biol Rhythm Res, 49, pp.29~38 (2018)[DOI]
- Kumar A, "Expression profiling of major heat shock protein genes during different seasons in cattle(Bos indicus)and buffalo(Bubalus bubalis)under tropical climatic condition", J Therm Biol, 51, pp.55~64 (2015)[DOI]
- Maibam U, "Seasonal variation in HSP70 expression and oxidative stress in skin of zebu(Tharparkar)and crossbred(Karan Fries)cattle under tropical climate", Biol Rhythm Res, 48, pp.647~661 (2017)[DOI]
- Umesh K. Shandilya, "Heat stress modulates differential response in skin fibroblast cells of native cattle (Bos indicus) and riverine buffaloes (Bubalus bubalis)", Bioscience Reports, 40(2) (2020), Portland Press Ltd.[DOI]
- Hu H, "The effect of heat stress on gene expression and synthesis of heat-shock and milk proteins in bovine mammary epithelial cells", Anim Sci J, 87, pp.84~91 (2016)[DOI]
- Llamas-Luceño LN, "Exposing dairy bulls to high temperature-humidity index during spermatogenesis compromises subsequent embryo development in vitro", Theriogenology, 141, pp.16~25 (2020)[DOI]
- ACCESS 9. 4 Interface to ADABAS : Reference, "SAS", pp.2013, SAS Institute Inc
- Akaike H, "A new look at the statistical model identification", IEEE Trans Automat Contr, 19, pp.716~723 (1974)[DOI]
- Schwarz G, "Estimating the dimension of a model", Ann Statist, 6, pp.461~464 (1978)[DOI]
- Pires BV, "Differential expression of heat shock protein genes associated with heat stress in Nelore and Caracu beef cattle", Livest Sci, 230, pp.103839~ (2019)[DOI]
- Hooper HB, "Heat loss efficiency and HSPs gene expression of Nellore cows in tropical climate conditions", Int J Biometeorol, 63, pp.1475~1486 (2019)[DOI]
- Kim WS, "Heat-shock proteins gene expression in peripheral blood mononuclear cells as an indicator of heat stress in beef calves", Animals, 10, pp.895~ (2020)[DOI]
- Deb R, "Effect of heat stress on the expression profile of Hsp90 among Sahiwal(Bos indicus)and Frieswal(Bos indicus × Bos taurus)breed of cattle : a comparative study", Gene, 536, pp.435~440 (2014)[DOI]
- LCI(Livestock Conservation Inc, "Patterns of transit losses" (1970), Livestock Conservation Inc