Antioxidant activity and metabolic regulation of sodium salicylate on goat sperm at low temperature
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초록
Objective: The purpose of this study was to explore the effect of sodium salicylate (SS) on semen preservation and metabolic regulation in goats.Methods: Under the condition of low temperature, SS was added to goat semen diluent to detect goat sperm motility, plasma membrane, acrosome, antioxidant capacity, mitochondrial membrane potential (MMP) and metabonomics.Results: The results show that at the 8th day of low-temperature storage, the sperm motility of the 20 μM SS group was 66.64%, and the integrity rates of the plasma membrane and acrosome were both above 60%, significantly higher than those of the other groups. The activities of catalase and superoxide dismutase in the sperm of the 20 μM SS group were significantly higher than those of the control group, the contents of reactive oxygen species and malondialdehyde were significantly lower than those in the control group, the MMP was significantly higher than that in the control group, and the contents of Ca<sup>2+</sup> and total cholesterol were significantly higher than those in the control group. Through metabonomics analysis, there were significant metabolic differences between the control group and the 20 μM SS group. Twenty of the most significant metabolic markers were screened, mainly involving five metabolic pathways, of which nicotinic acid and nicotinamide metabolic pathways were the most significant.Conclusion: The results indicate that SS can effectively improve the low-temperature preservation quality of goat sperm.
Abstract
Objective: The purpose of this study was to explore the effect of sodium salicylate (SS) on semen preservation and metabolic regulation in goats. Methods: Under the condition of low temperature, SS was added to goat semen diluent to detect goat sperm motility, plasma membrane, acrosome, antioxidant capacity, mitochondrial membrane potential (MMP) and metabonomics. Results: The results show that at the 8th day of low-temperature storage, the sperm motility of the 20 μM SS group was 66.64%, and the integrity rates of the plasma membrane and acrosome were both above 60%, significantly higher than those of the other groups. The activities of catalase and superoxide dismutase in the sperm of the 20 μM SS group were significantly higher than those of the control group, the contents of reactive oxygen species and malondialdehyde were significantly lower than those in the control group, the MMP was significantly higher than that in the control group, and the contents of Ca2+ and total cholesterol were significantly higher than those in the control group. Through metabonomics analysis, there were significant metabolic differences between the control group and the 20 μM SS group. Twenty of the most significant metabolic markers were screened, mainly involving five metabolic pathways, of which nicotinic acid and nicotinamide metabolic pathways were the most significant. Conclusion: The results indicate that SS can effectively improve the low-temperature preservation quality of goat sperm.
참고문헌 (40)
- Alvarenga, "Advances in stallion semen cryopreservation", Vet Clin North Am Equine Pract, 32, pp.521~530 (2016)[DOI]
- Yanez-Ortiz I, "Advances in sperm cryopreservation in farm animals : Cattle, horse, pig and sheep", Anim Reprod Sci, 246, pp.106904~ (2022)[DOI]
- Li Q, "Effects of oligomeric proanthocyanidins on quality of boar semen during liquid preservation at 17 degrees C", Anim Reprod Sci, 198, pp.47~56 (2018)[DOI]
- Shaoyong W, "Effects of kojic acid on boar sperm quality and anti-bacterial activity during liquid preservation at 17C", Theriogenology, 140, pp.124~135 (2019)[DOI]
- Tian X, "Supplementation of salvianic acid A to boar semen extender to improve seminal quality and antioxidant capacity", Anim Sci J, 90, pp.1142~1148 (2019)[DOI]
- Madunic J, "Sodium salicylate inhibits urokinase activity in MDA MB-231 breast cancer cells", Clin Breast Cancer, 17, pp.629~637 (2017)[DOI]
- Odebiyi DO, "Effect of sodium salicylate iontophoresis in the management of hip pain in patients with sickle cell disease", Nig Q J Hosp Med, 17, pp.82~86 (2007)[DOI]
- Kazama I, "Amphipaths differentially modulate membrane surface deformation in rat peritoneal mast cells during exocytosis", Cell Physiol Biochem, 31, pp.592~600 (2013)[DOI]
- Eidan SM, "Effect on post-cryopreserved semen characteristics of Holstein bulls of adding combinations of vitamin C and either catalase or reduced glutathione to Tris extender", Anim Reprod Sci, 167, pp.1~7 (2016)[DOI]
- Hardie DG, "AMP-activated protein kinase : a target for drugs both ancient and modern", Chem Biol, 19, pp.1222~1236 (2012)[DOI]
- Feng TY, "Rosmarinic acid improves boar sperm quality, antioxidant capacity and energy metabolism at 17 degrees C via AMPK activation", Reprod Domest Anim, 55, pp.1714~1724 (2020)[DOI]
- Martin-Hidalgo D, "AMPK function in mammalian spermatozoa", Int J Mol Sci, 19, pp.3239~ (2018)[DOI]
- Hawley SA, "The ancient drug salicylate directly activates AMP-activated protein kinase", Science, 336, pp.918~922 (2012)[DOI]
- Dasgupta B, "AMPK. Methods in Molecular Biology vol 1732", pp.195~202 (2018), Humana Press
- Vasquez J, "Hypoosmotic swelling test in epididymal ram(Ovis aries)spermatozoa", Livest Sci, 157, pp.618~622 (2013)[DOI]
- Selvaraju S, "Deciphering the complexity of sperm transcriptome reveals genes governing functional membrane and acrosome integrities potentially influence fertility", Cell Tissue Res, 385, pp.207~222 (2021)[DOI]
- Ren F, "Lycium barbarum and Laminaria japonica polysaccharides improve Cashmere goat sperm quality and fertility rate after cryopreservation", Theriogenology, 129, pp.29~36 (2019)[DOI]
- Xu D, "Rutin protects boar sperm from cryodamage via enhancing the antioxidative defense", Anim Sci J, 91, pp.e13328~ (2020)[DOI]
- Karaji RO, "Effects of in combination antioxidant supplementation on microscopic and oxidative parameters of freeze-thaw bull sperm", Cell Tissue Bank, 15, pp.461~470 (2014)[DOI]
- Uribe P, "Use of the fluorescent dye tetramethylrhodamine methyl ester perchlorate for mitochondrial membrane potential assessment in human spermatozoa", Andrologia, 49, pp.e12753~ (2017)[DOI]
- Zhu Z, "Negative effects of ROS generated during linear sperm motility on gene expression and ATP generation in boar sperm mitochondria", Free Radic Biol Med, 141, pp.159~171 (2019)[DOI]
- Lesich KA, "The calcium response of mouse sperm flagella : role of calcium ions in the regulation of dynein activity", Biol Reprod, 86, pp.105~ (2012)[DOI]
- El-Seadawy IE, "The impact of varying doses of moringa leaf methanolic extract supplementation in the cryopreservation media on sperm quality, oxidants, and antioxidant capacity of frozen-thawed ram sperm", Trop Anim Health Prod, 54, pp.344~ (2022)[DOI]
- Ai J, "Using untargeted metabolomics to profile the changes in roselle(Hibiscus sabdariffa L. )anthocyanins during wine fermentation", Food Chem, 364, pp.130425~ (2021)[DOI]
- Li C, "Changes on proteomic and metabolomic profiling of cryopreserved sperm effected by melatonin", J Proteomics, 273, pp.104791~ (2023)[DOI]
- Akbari E, "Comparative investigation of analgesic tolerance to taurine, sodium salicylate and morphine : involvement of peripheral muscarinic receptors", Neurosci Lett, 795, pp.137041~ (2023)[DOI]
- Koga T, "Effects of food preservatives and local anesthetics on synthesis of outer membrane proteins in Vibrio parahaemolyticus", Tokushima J Exp Med, 36, pp.41~45 (1989)
- Marson FG, "Sodium salicylate and probenecid in the treatment of chronic gout; assessment of their relative effects in lowering serum uric acid levels", Ann Rheum Dis, 13, pp.233~245 (1954)[DOI]
- Tsai TY, "Suppression of Ca2+ influx in endotoxin-treated mouse cerebral cortex endothelial bEND. 3 cells", Eur J Pharmacol, 755, pp.80~87 (2015)[DOI]
- Lim SC, "Sodium salicylate switches glucose depletion-induced necrosis to autophagy and inhibits high mobility group box protein 1 release in A549 lung adenocarcinoma cells", Oncol Rep, 19, pp.1165~1171 (2008)[DOI]
- Bao W, "Sodium salicylate modulates inflammatory responses through AMP-activated protein kinase activation in LPS-stimulated THP-1 cells", J Cell Biochem, 119, pp.850~860 (2018)[DOI]
- Li RN, "Metformin improves boar sperm quality via 5 '-AMP-activated protein kinase-mediated energy metabolism in vitro", Zool Res, 41, pp.527~538 (2020)[DOI]
- Hurtado de Llera A, "AMPK up-activation reduces motility and regulates other functions of boar spermatozoa", Mol Hum Reprod, 21, pp.31~45 (2015)[DOI]
- Zhu Z, "Resveratrol improves boar sperm quality via 5 ' AMP-activated protein kinase activation during cryopreservation", Oxid Med Cell Longev, 2019, pp.5921503~ (2019)[DOI]
- Wang W, "High doses of salicylate and aspirin are inhibitory on acid-sensing ion channels and protective against acidosis-induced neuronal injury in the rat cortical neuron", J Neurosci Res, 90, pp.267~277 (2012)[DOI]
- Khandelia H, "Interaction of salicylate and a terpenoid plant extract with model membranes : reconciling experiments and simulations", Biophys J, 99, pp.3887~3894 (2010)[DOI]
- Gleitsman KR, "Structural rearrangements of the motor protein prestin revealed by fluorescence resonance energy transfer", Am J Physiol Cell Physiol, 297, pp.C290~C298 (2009)[DOI]
- Hopp AK, "Regulation of glucose metabolism by NAD(+)and ADP-ribosylation", Cells, 8, pp.890~ (2019)[DOI]
- Ma D, "Nicotinamide mononucleotide improves spermatogenic function in streptozotocin-induced diabetic mice via modulating the glycolysis pathway", Acta Biochim Biophys Sin, 54, pp.1314~1324 (2022)[DOI]
- Torres MA, "Metabolomic signature of spermatozoa established during holding time is responsible for differences in boar sperm freezability", Biol Reprod, 106, pp.213~226 (2022)[DOI]
