Alpha toxin production potential and antibiotic resistance patterns of clostridium perfringens isolates from meat samples
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objective: This research aimed to analyze the prevalence, molecular characteristics, toxinotyping, alpha toxin production potential, and antibiotic resistance pattern of <i>Clostridium perfringens</i> (<i>C. perfringens</i>) isolates in meat samples collected from various sources.Methods: Sixty meat samples were screened for alpha toxin using enzyme-linked immunosorbent assay, revealing a positivity rate of 13.3%, predominantly in raw poultry meat. Subsequent culturing on Perfringens agar identified nine samples harboring characteristic <i>C. perfringens</i> colonies, primarily isolated from raw poultry meat. Molecular confirmation through 16S rRNA gene amplification and sequencing authenticated twelve isolates as C. perfringens, with nine strains exhibiting genetic resemblance to locally isolated strains. Toxinotyping assays targeting alpha toxin-specific genes confirmed all nine isolates as type A <i>C. perfringens</i>, with no detection of beta or epsilon toxin genes. Hemolytic assays demonstrated varying alpha toxin production potentials among isolates, with accession number OQ721004.1 displaying the highest production capacity. Moreover, antibiotic resistance profiling revealed multi-drug resistance patterns among the isolates.Results: The study identified distinct clusters within <i>C. perfringens</i> strains, indicating variations. Phylogenetic analysis delineated genetic relatedness among strains, elucidating potential evolutionary paths and divergences.Conclusion: The findings underscore the need for robust surveillance and control measures to mitigate the risk of <i>C. perfringens</i> contamination in meat products, particularly in raw poultry meat. Enhanced monitoring and prudent antimicrobial stewardship practices are warranted in both veterinary and clinical settings to address the observed antibiotic resistance profiles and prevent foodborne outbreaks.
Abstract
Objective: This research aimed to analyze the prevalence, molecular characteristics, toxinotyping, alpha toxin production potential, and antibiotic resistance pattern of Clostridium perfringens (C. perfringens) isolates in meat samples collected from various sources. Methods: Sixty meat samples were screened for alpha toxin using enzyme-linked immunosorbent assay, revealing a positivity rate of 13.3%, predominantly in raw poultry meat. Subsequent culturing on Perfringens agar identified nine samples harboring characteristic C. perfringens colonies, primarily isolated from raw poultry meat. Molecular confirmation through 16S rRNA gene amplification and sequencing authenticated twelve isolates as C. perfringens, with nine strains exhibiting genetic resemblance to locally isolated strains. Toxinotyping assays targeting alpha toxin-specific genes confirmed all nine isolates as type A C. perfringens, with no detection of beta or epsilon toxin genes. Hemolytic assays demonstrated varying alpha toxin production potentials among isolates, with accession number OQ721004.1 displaying the highest production capacity. Moreover, antibiotic resistance profiling revealed multi-drug resistance patterns among the isolates. Results: The study identified distinct clusters within C. perfringens strains, indicating variations. Phylogenetic analysis delineated genetic relatedness among strains, elucidating potential evolutionary paths and divergences. Conclusion: The findings underscore the need for robust surveillance and control measures to mitigate the risk of C. perfringens contamination in meat products, particularly in raw poultry meat. Enhanced monitoring and prudent antimicrobial stewardship practices are warranted in both veterinary and clinical settings to address the observed antibiotic resistance profiles and prevent foodborne outbreaks.
참고문헌 (25)
- Freedman JC, "Clostridium perfringens enterotoxin : action, genetics, and translational applications", Toxins, 8, pp.73~ (2016)[DOI]
- Bendary MM, "Clostridium perfringens associated with foodborne infection of animal origins : insights into prevalence, antimicrobial resistance, toxin genes profiles, and toxinotypes", Biology, 11, pp.551~ (2022)[DOI]
- Zafar Khan MU, "Infection dynamics of clostridium perfringens fingerprinting in buffalo and cattle of punjab province, pakistan", Front Vet Sci, 9, pp.762449~ (2022)[DOI]
- Kiu R, "An update on the human and animal enteric pathogen Clostridium perfringens", Emerg Microbes Infect, 7, pp.1~15 (2018)[DOI]
- Bhattacharya A, "Outbreak of Clostridium perfringens food poisoning linked to leeks in cheese sauce : an unusual source", Epidemiol Infect, 148, pp.e43~ (2020)[DOI]
- Edwards AN, "Chemical and stress resistances of Clostridium difficile spores and vegetative cells", Front Microbiol, 7, pp.1698~ (2016)[DOI]
- Shrestha A, "Enterotoxic clostridia : Clostridium perfringens enteric diseases", Microbiol Spectr, 6, pp.17~ (2018)[DOI]
- Hassani S, "Prevalence, antibiotic resistance, toxin-typing and genotyping of Clostridium perfringens in raw beef meats obtained from qazvin city, Iran", Antibiotics, 11, pp.340~ (2022)[DOI]
- El Kadri H, "The effect of low-temperature long-time(LTLT)cooking on survival of potentially pathogenic Clostridium perfringens in beef", Int J Food Microbiol, 320, pp.108540~ (2020)[DOI]
- Rana EA, "Phenotypical identification and toxinotyping of Clostridium perfringens isolates from healthy and enteric disease-affected chickens", Vet Med Int, 2023, pp.2584171~ (2023)[DOI]
- Mohiuddin M, "Animal model studies, antibiotic resistance and toxin gene profile of NE reproducing Clostridium perfringens Type A and Type G strains isolated from commercial poultry farms in China", Microorganisms, 11, pp.622~ (2023)[DOI]
- Hamza D, "Toxinotyping and antimicrobial resistance of Clostridium perfringens isolated from processed chicken meat products", J Vet Res, 61, pp.53~58 (2017)[DOI]
- Kuo J, "Toxigenic Clostridium perfringens isolated from at-risk pediatric inflammatory bowel disease patients", J Crohns Colitis, 20, pp.jjae016~ (2024)[DOI]
- Talukdar PK, "Identification of ger minants and expression of germination genes in Clostridium perfringens strains isolated from diarrheic animals", Pathogens, 13, pp.194~ (2024)[DOI]
- Fayez M, "Genotyping and antimicrobial susceptibility of Clostridium perfringens and Clostridioides difficile in camel minced meat", Pathogens, 10, pp.1640~ (2021)[DOI]
- Yang T, "Effects of Clostridium butyricum as an antibiotic alternative on growth performance, intestinal morphology, serum biochemical response, and immunity of broilers", Antibiotics, 12, pp.433~ (2023)[DOI]
- Haider Z, "Isolation, toxinotyping and antimicrobial susceptibility testing of Clostridium perfringens isolated from Pakistan poultry", Anaerobe, 73, pp.102499~ (2022)[DOI]
- Mohamed HMA, "Molecular characterization of Clostridium perfringens in small ruminants", SVU Int J Vet Sci, 6, pp.104~123 (2023)[DOI]
- Hassan KA, "Genomic analyses of Clostridium perfringens isolates from five toxinotypes", Res Microbiol, 166, pp.255~263 (2015)[DOI]
- Schneider D, "Peyronie's disease response to intralesional collagenase clostridium histolyticum therapy is independent of baseline testosterone", Andrology, 12, pp.830~834 (2023)[DOI]
- Feng X, "Effects of challenge with Clostridium perfringens, Eimeria and both on ileal microbiota of yellow feather broilers", Front Microbiol, 13, pp.1063578~ (2022)[DOI]
- Praveen Kumar N, "Molecular detection and characterization of Clostridium perfringens toxin genes causing necrotic enteritis in broiler chickens", Trop Anim Health Prod, 51, pp.1559~1569 (2019)[DOI]
- Anju K, "Toxinotyping and molecular characterization of antimicrobial resistance in Clostridium perfringens isolated from different sources of livestock and poultry", Anaerobe, 67, pp.102298~ (2021)[DOI]
- Mohiuddin M, "Prevalence, genotypic and phenotypic characterization and antibiotic resistance profile of Clostridium perfringens Type A and D isolated from feces of sheep(Ovis aries)and goats(Capra hircus)in Punjab, Pakistan", Toxins, 12, pp.657~ (2020)[DOI]
- Yadav JP, "Prevalence, molecular characterization, and antimicrobial resistance profile of Clostridium perfringens from India : a scoping review", Anaerobe, 77, pp.102639~ (2022)[DOI]