본문 바로가기
KERD
논문

Complete genome sequence of Clostridium perfringens B20, a bacteriocin-producing pathogen

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류축산학

초록

Clostridium perfringens B20 was isolated from chicken feces collected from a local farm associated with Chung-Ang University (Anseong, Korea). The whole genome of C. perfringens B20 was sequenced using the PacBio RS II platform and assembled de novo. The genome is 2,982,563 bp long and assembled in two contigs. Annotation analyses revealed 2,668 protein-coding sequences, 30 rRNA genes, and 94 tRNA genes, with 28.2% G + C (guanine + cytosine) content. In silico genomic analysis revealed the presence of genes encoding a class IId bacteriocin, lactococcin A, and associated ABC transporter and immunity proteins, as well as a putative bacteriocin gene.

Abstract

Clostridium perfringens B20 was isolated from chicken feces collected from a local farm associated with Chung-Ang University (Anseong, Korea). The whole genome of C. perfringens B20 was sequenced using the PacBio RS II platform and assembled de novo. The genome is 2,982,563 bp long and assembled in two contigs. Annotation analyses revealed 2,668 protein-coding sequences, 30 rRNA genes, and 94 tRNA genes, with 28.2% G + C (guanine + cytosine) content. In silico genomic analysis revealed the presence of genes encoding a class IId bacteriocin, lactococcin A, and associated ABC transporter and immunity proteins, as well as a putative bacteriocin gene.

참고문헌 (10)

  1. P.F. Cuevas-González, "Postbiotics and paraprobiotics: From concepts to applications", Food Research International, 136, pp.109502~ (2020), Elsevier BV[DOI]
  2. Mehran Moradi, "Postbiotics produced by lactic acid bacteria: The next frontier in food safety", Comprehensive Reviews in Food Science and Food Safety, 19(6), pp.3390~3415 (2020), Wiley[DOI]
  3. Zuamí Villagrán-de la Mora, "Clostridium perfringens as Foodborne Pathogen in Broiler Production: Pathophysiology and Potential Strategies for Controlling Necrotic Enteritis", Animals, 10(9), pp.1718~ (2020), MDPI AG[DOI]
  4. Raymond Kiu, "Probing Genomic Aspects of the Multi-Host Pathogen Clostridium perfringens Reveals Significant Pangenome Diversity, and a Diverse Array of Virulence Factors", Frontiers in Microbiology, 8, pp.2485~ (2017), Frontiers Media SA[DOI]
  5. 허선학, "Inhibition of Clostridium perfringens using Bacteriophages and Bacteriocin Producing Strains", 한국축산식품학회지, 38(1), pp.88~98 (2018), 한국축산식품학회
  6. Patricia P. Chan, "tRNAscan-SE: Searching for tRNA Genes in Genomic Sequences", Methods in Molecular Biology, 1962, pp.1~14 (2019), Springer New York[DOI]
  7. E. P. Nawrocki, "Infernal 1.1: 100-fold faster RNA homology searches", Bioinformatics, 29(22), pp.2933~2935 (2013), Oxford University Press (OUP)[DOI]
  8. Doug Hyatt, "Prodigal: prokaryotic gene recognition and translation initiation site identification", BMC Bioinformatics, 11(1), pp.119~ (2010), Springer Science and Business Media LLC[DOI]
  9. Jeella Z Acedo, "The expanding structural variety among bacteriocins from Gram-positive bacteria", FEMS Microbiology Reviews, 42(6), pp.805~828 (2018), Oxford University Press (OUP)[DOI]
  10. H Holo, "Lactococcin A, a new bacteriocin from Lactococcus lactis subsp. cremoris: isolation and characterization of the protein and its gene", Journal of Bacteriology, 173(12), pp.3879~3887 (1991), American Society for Microbiology[DOI]

관련 논문AI추천

이 간행물의 다른 논문 (22건)