Isolation of a Potential Probiotic Levilactobacillus brevis and Evaluation of Its Exopolysaccharide for Antioxidant and α-Glucosidase Inhibitory Activities
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The probiotic properties of ten lactic acid bacteria and antioxidant and α-glucosidase inhibitory activities of the exopolysaccharide (EPS) of the selected strain were investigated in this study. Levilactobacillus brevis L010 was one of the most active strains across all the in vitro tests. The cellfree supernatant (50 g/l) of L. brevis L010 showed high levels of both α-glucosidase inhibitory activity (98.73 ± 1.32%) and 2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity (32.29 ± 3.86%). The EPS isolated from cell-free supernatant of L. brevis L010 showed 2,2'-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid) radical-scavenging activity (80.27 ± 2.51%) at 80 g/l, DPPH radical-scavenging activity (38.19 ± 9.61%) at 40 g/l, and ferric reducing antioxidant power (17.35 ± 0.20 mg/l) at 80 g/l. Further, EPS exhibited inhibitory activities against α-glucosidase at different substrate concentrations. Kinetic analysis suggests that the mode of inhibition was competitive, with a kinetic constant of Km = 2.87 ± 0.88 mM and Vmax = 0.39 ± 0.06 μmole/min. It was concluded that the EPS might be one of the plausible candidates for possible antioxidant and α-glucosidase activities of the L. brevis L010 strain.
Abstract
The probiotic properties of ten lactic acid bacteria and antioxidant and α-glucosidase inhibitory activities of the exopolysaccharide (EPS) of the selected strain were investigated in this study. Levilactobacillus brevis L010 was one of the most active strains across all the in vitro tests. The cellfree supernatant (50 g/l) of L. brevis L010 showed high levels of both α-glucosidase inhibitory activity (98.73 ± 1.32%) and 2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity (32.29 ± 3.86%). The EPS isolated from cell-free supernatant of L. brevis L010 showed 2,2'-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid) radical-scavenging activity (80.27 ± 2.51%) at 80 g/l, DPPH radical-scavenging activity (38.19 ± 9.61%) at 40 g/l, and ferric reducing antioxidant power (17.35 ± 0.20 mg/l) at 80 g/l. Further, EPS exhibited inhibitory activities against α-glucosidase at different substrate concentrations. Kinetic analysis suggests that the mode of inhibition was competitive, with a kinetic constant of Km = 2.87 ± 0.88 mM and Vmax = 0.39 ± 0.06 μmole/min. It was concluded that the EPS might be one of the plausible candidates for possible antioxidant and α-glucosidase activities of the L. brevis L010 strain.
참고문헌 (47)
- Guidelines for the evaluation of probiotics in food, "Araya M", joint FAO, 2002
- Jang MH, "β-1, 4-Xylosidase activity of Leuconostoc lactic acid bacteria isolated from kimchi", Korean J. Food Sci. Technol, 43, pp.169~175 (2011)
- Park YU, "Probiotic properties of lactic acid bacteria isolated from Korean rice wine Makgeolli", Food Sci. Biotechnol, 24, pp.1761~1766 (2015)
- Min KA, "Yogurt production using exo-polysaccharide-producing Leuconostoc and Weissella isolates from kimchi", Korean J. Food Sci. Technol, 48, pp.231~240 (2016)
- Yu HS, "Evaluation of the probiotic characteristics and prophylactic potential of Weissella cibaria strains isolated from kimchi", LWT, 112, pp.108229~ (2019)
- Soares MB, "The resistance of Bacillus, Bifidobacterium, and Lactobacillus strains with claimed probiotic properties in different food matrices exposed to simulated gastrointestinal tract conditions", Food Res. Int, 112, pp.108229~ (2019)
- Cavalcante RGS, "The probiotic Lactobacillus fermentum 296 attenuates cardiometabolic disorders in high fat diet-treated rats", Nutr. Metab. Cardiovasc. Dis, 29, pp.1408~1417 (2019)
- Lee SR, "Leuconostoc mesenteroides MKSR isolated from kimchi possesses α-glucosidase inhibitory activity, antioxidant activity, and cholesterol-lowering effects", LWT, 116, pp.108570~ (2019)
- Lim SM, "Lactobacillus sakei OK67 ameliorates high-fat diet–induced blood glucose intolerance and obesity in mice by inhibiting gut microbiota lipopolysaccharide production and inducing colon tight junction protein expression", Nutr. Res, 36, pp.337~348 (2016)
- Son SH, "In vitro characterization of Lactobacillus brevis KU15006, an isolate from kimchi, reveals anti-adhesion activity against foodborne pathogens and antidiabetic properties", Microb. Pathog, 112, pp.135~141 (2017)
- Kechagia M, "Health benefits of probiotics : a review", ISRN Nutr, 2, pp.481651~ (2013)
- Song MW, "Probiotic and antioxidant properties of novel Lactobacillus brevis KCCM 12203P isolated from kimchi and evaluation of immune-stimulating activities of its heat-killed cells in RAW 264.7 cells", J. Microbiol. Biotechnol, 29, pp.1894~1903 (2019)
- Argyri AA, "Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests", Food Microbiol, 33, pp.282~291 (2013)
- Chen P, "Screening for potential new probiotic based on probiotic properties and α-glucosidase inhibitory activity", Food Control, 35, pp.65~72 (2014)
- Oboh G, "Influence of gallic acid on α-amylase and α-glucosidase inhibitory properties of acarbose", J. Food Drug Anal, 24, pp.627~634 (2016)
- Chiasson JL, "Acarbose for prevention of type 2 diabetes mellitus : the STOP-NIDDM randomised trial", Lancet, 359, pp.2072~2077 (2002)
- Ron Y, "The effect of acarbose on the colonic transit time of elderly long-term care patients with type 2 diabetes mellitus", J. Gerontol. A. Biol. Sci. Med. Sci, 57, pp.111~114 (2002)
- Zeng Z, "Screening for potential novel probiotic Lactobacillus strains based on high dipeptidyl peptidase IV and α-glucosidase inhibitory activity", J. Funct. Foods, 20, pp.486~495 (2016)
- Ramchandran L, "Effect of exopolysaccharides and inulin on the proteolytic, angiotensin-I-converting enzyme-and α-glucosidase-inhibitory activities as well as on textural and rheological properties of low-fat yogurt during refrigerated storage", J. Dairy Sci, 89, pp.583~600 (2009)
- Kwun SY, "Isolation of acid tolerant lactic acid bacteria and evaluation of α-glucosidase inhibitory activity", Food Sci. Biotechnol, 29, pp.1125~1130 (2020)
- Kachouri F, "Involvement of antioxidant activity of Lactobacillus plantarum on functional properties of olive phenolic compounds", J. Food Sci. Technol, 52, pp.7924~7933 (2015)
- Tuomola EM, "Adhesion of some probiotic and dairy Lactobacillus strains to Caco-2 cell cultures", Int. J. Food Microbiol, 5, pp.45~51 (1998)
- Maturin L, "BAM: aerobic plate count. Bacteriological Analytical Manual", FDA, ~ (2001)
- Hassanzadazar H, "Investigation of antibacterial, acid and bile tolerance properties of lactobacilli isolated from Koozeh cheese", Vet. Res. Forum, 3, pp.181~185 (2012)
- Park EH, "Antipathogenic activity of Lactobacillus plantarum isolated from pickled mulberry leaf", Microbiol. Biotechnol. Lett, 44, pp.163~170 (2016)
- De S, "A simple method for the efficient isolation of genomic DNA from lactobacilli isolated from traditional Indian fermented milk(dahi)", Indian J. Microbiol, 50, pp.412~418 (2010)
- Kumar S, "MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets", Mol. Biol. Evol, 33, pp.1870~1874 (2016)
- Berney M, "Specific growth rate determines the sensitivity of Escherichia coli to thermal, UVA, and solar disinfection", ASM, 72, pp.2586~2593 (2006)
- Sharma K, "Purification and characterization of novel exopolysaccharides produced from Lactobacillus paraplantarum KM1 isolated from human milk and its cytotoxicity", J. Genet. Eng. Biotechnol, 18, pp.1~10 (2020)
- Oh NS, "Preventive effect of fermented maillard reaction products from milk proteins in cardiovascular health", J. Dairy Sci, 97, pp.3300~3313 (2014)
- Nurcholis W, "Phytochemical analysis, α-glucosidase inhibition activity in-vitro and enzyme kinetics of ethyl acetate and hexane extracts of Graptophylum pictum (L.) Griff", Curr. Biochem, 1, pp.58~65 (2014)
- Lineweaver H, "The determination of enzyme dissociation constants", J. Am. Chem. Soc, 56, pp.658~666 (1934)
- Duncan DB, "Multiple range and multiple F tests", Biometrics, 11, pp.1~42 (1955)
- Nurhayati R, "Lactic acid bacteria producing inhibitor of alpha glucosidase isolated from ganyong(Canna Edulis)and kimpul(Xanthosoma sagittifolium)", IOP Conf. Ser. Earth Environ. Sci, 101, pp.0122009~ (2017)
- Oh NS, "Probiotic and anti-inflammatory potential of Lactobacillus rhamnosus 4B15 and Lactobacillus gasseri 4M13 isolated from infant feces", PLoS One, 13, pp.e0192021~ (2018)
- Lai YJ, "Isolation of exopolysaccharide producing Lactobacillus strains from sorghum distillery residues pickled cabbage and their antioxidant properties", Food Sci. Biotechnol, 23, pp.1231~1234 (2014)
- Mulaw G, "In vitro evaluation of probiotic properties of lactic acid bacteria isolated from some traditionally fermented Ethiopian food products", Int. J. Microbiol, 2019, pp.7179514~ (2019)
- Jang HJ, "Probiotic characterization of Lactobacillus brevis KU15153 showing antimicrobial and antioxidant effect isolated from kimchi", Food Sci. Biotechnol, 28, pp.1521~1528 (2019)
- Śliżewska K, "Growth kinetics of probiotic Lactobacillus strains in the alternative, cost-efficient semi-solid fermentation medium", Biology, 9, pp.423~ (2020)
- Ruiz Rodríguez LG, "Diversity and functional properties of lactic acid bacteria isolated from wild fruits and flowers present in northern Argentina", Front. Microbiol, 10, pp.1091~ (2019)
- Guérin M, "Lactic acid bacterial production of exopolysaccharides from fruit and vegetables and associated benefits", Fermentation, 6, pp.115~ (2020)
- Nguyen PT, "Exopolysaccharide production by lactic acid bacteria : the manipulation of environmental stresses for industrial applications", AIMS Microbiol, 6, pp.451~469 (2020)
- Bomfim VB, "Partial characterization and antioxidant activity of exopolysaccharides produced by Lactobacillus plantarum CNPC003", LWT, 127, pp.103949~ (2020)
- Zhang BW, "Pentacyclic triterpenes as a-glucosidase and a-amylase inhibitors : structure-activity relationships and the synergism with acarbose", Bioorganic Med. Chem. Lett, 27, pp.5065~5070 (2017)
- Son HU, "Comparison of α-glucosidase inhibition by Cudrania tricuspidata according to harvesting time", Biomed. Rep, 1, pp.624~628 (2013)
- Bajpai V, "Partially purified exo-polysaccharide from Lactobacillus sakei Probio 65 with antioxidant, α-glucosidase and tyrosinase inhibitory potential", J. Food Biochem, 40, pp.264~274 (2016)
- Saitou N, "The neighbor-joining method : a new method for reconstructing phylogenetic trees", Mol. Biol. Evol, 4, pp.406~425 (1987)
