Identification of polymorphic loci in the deiodinase 2 gene and their associations with head dimensions in geese
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objective: This study was conducted to clone and compare the molecular characteristics of the deiodinase 2 (DIO2) gene between Sichuan White geese and Landes geese, and to analyze the association between polymorphisms of the DIO2 gene and head dimensions in Tianfu meat geese. Methods: The coding sequence of the DIO2 gene was cloned by polymerase chain reaction and vector ligation and aligned by DNAMAN software. A total of 350 Tianfu meat geese were used to genotype the polymorphisms of the DIO2 gene and measure the head dimensions. Association analysis between the polymorphisms of the DIO2 gene and head dimensions was carried out. Results: An 840-bp coding sequence of the DIO2 gene was obtained and comparison analysis identified four polymorphic loci between Sichuan White geese and Landes geese. Further analysis showed that the dominant alleles for the four polymorphic loci were G, G, A, and T and the frequency of the heterozygous genotype was higher than that of the homozygous genotype in Tianfu meat geese. Compared to that in the population of non-knob geese of Tianfu meat geese, the head dimensions in the population of knob geese were significantly higher except for nostril height. However, in the non-knob geese, beak width 1, beak width 2, nostril length, cranial width 1, and maxillary length had significant differences among different genotypes or haplotypes/diplotypes. Conclusion: These results suggested that polymorphisms of the DIO2 gene could be considered molecular markers to select larger heads of geese in the population of non-knob geese.
Abstract
Objective: This study was conducted to clone and compare the molecular characteristics of the deiodinase 2 (DIO2) gene between Sichuan White geese and Landes geese, and to analyze the association between polymorphisms of the DIO2 gene and head dimensions in Tianfu meat geese. Methods: The coding sequence of the DIO2 gene was cloned by polymerase chain reaction and vector ligation and aligned by DNAMAN software. A total of 350 Tianfu meat geese were used to genotype the polymorphisms of the DIO2 gene and measure the head dimensions. Association analysis between the polymorphisms of the DIO2 gene and head dimensions was carried out. Results: An 840-bp coding sequence of the DIO2 gene was obtained and comparison analysis identified four polymorphic loci between Sichuan White geese and Landes geese. Further analysis showed that the dominant alleles for the four polymorphic loci were G, G, A, and T and the frequency of the heterozygous genotype was higher than that of the homozygous genotype in Tianfu meat geese. Compared to that in the population of non-knob geese of Tianfu meat geese, the head dimensions in the population of knob geese were significantly higher except for nostril height. However, in the non-knob geese, beak width 1, beak width 2, nostril length, cranial width 1, and maxillary length had significant differences among different genotypes or haplotypes/diplotypes. Conclusion: These results suggested that polymorphisms of the DIO2 gene could be considered molecular markers to select larger heads of geese in the population of non-knob geese.
참고문헌 (30)
- Bianco AC, "The deiodinase trio and thyroid hormone signaling. In : Plateroti M, Samarut J, editors. Thyroid hormone nuclear receptor", Methods Mol Biol, 1801, pp.67~83 (2018)[DOI]
- Marsili A, "Mice with a targeted deletion of the type 2 deiodinase are insulin resistant and susceptible to diet induced obesity", PLoS One, 6, pp.e20832~ (2011)[DOI]
- Waung JA, "Adult mice lacking the type 2 iodothyronine deiodinase have increased subchondral bone but normal articular cartilage", Thyroid, 25, pp.269~277 (2015)[DOI]
- Bassett JHD, "Optimal bone strength and mineralization requires the type 2 iodothyronine deiodinase in osteoblasts", Proc Natl Acad Sci USA, 107, pp.7604~7609 (2010)[DOI]
- Meulenbelt I, "Identification of DIO2 as a new susceptibility locus for symptomatic osteoarthritis", Hum Mol Genet, 17, pp.1867~1875 (2008)[DOI]
- Guo TW, "Positive association of the DIO2 (deiodinase type 2) gene with mental retardation in the iodine-deficient areas of China", J Med Genet, 41, pp.585~590 (2004)[DOI]
- Zhang K, "A family-based association study of DIO2 and children mental retardation in the Qinba region of China", J Hum Genet, 57, pp.14~17 (2012)[DOI]
- Heemstra KA, "The type 2 deiodinase Thr92Ala polymorphism is associated with increased bone turnover and decreased femoral neck bone mineral density", J Bone Miner Res, 25, pp.1385~1391 (2010)[DOI]
- Uter JC, "Single nucleotide polymorphisms in thyroid hormone transporter genes MCT8, MCT10 and deiodinase DIO2 contribute to inter-individual variance of executive functions and personality traits", Ex Clin Endocrinol Diabetes, 128, pp.573~581 (2020)[DOI]
- Leitch VD, "Role of thyroid hormones in craniofacial development", Nat Rev Endocrinol, 16, pp.147~164 (2020)[DOI]
- Bakker B, "Two decades of screening for congenital hypothyroidism in The Netherlands : TPO gene mutations in total iodide organification defects(an update)", J Clin Endocrinol Metab, 85, pp.3708~3712 (2000)[DOI]
- Macchia PE, "PAX8 mutations associated with congenital hypothyroidism caused by thyroid dysgenesis", Nat Genet, 19, pp.83~86 (1998)[DOI]
- Ji W, "Identifying molecular pathways and candidate genes associated with knob traits by transcriptome analysis in the goose (Anser cygnoides)", Sci Rep, 11, pp.11978~ (2021)[DOI]
- Deng Y, "Integrative analysis of histomorphology, transcriptome and whole genome resequencing identified DIO2 gene as a crucial gene for the protuberant knob located on forehead in geese", BMC Genomics, 22, pp.487~ (2021)[DOI]
- Croteau W, "Cloning of the mammalian type II iodothyronine deiodinase. A selenoprotein differentially expressed and regulated in human and rat brain and other tissues", J Clin Invest, 98, pp.405~417 (1996)[DOI]
- Gereben B, "Cloning and expression of the chicken type 2 iodothyronine 5'-deiodinase", J Biol Chem, 274, pp.13768~13776 (1999)[DOI]
- Song S, "Biochemical and molecular biological evidence for the presence of type II iodothyronine deiodinase in mouse mammary gland", Mol Cell Endocrinol, 160, pp.173~181 (2000)[DOI]
- Connor EE, "Molecular cloning, expression and radiation hybrid mapping of the bovine deiodinase type II (DIO2) and deiodinase type III (DIO3) genes", Anim Genet, 36, pp.240~243 (2005)[DOI]
- Robinson MR, "Live fast, die young : trade-offs between fitness components and sexually antagonistic selection on weaponry in Soay sheep", Evolution, 60, pp.2168~2181 (2006)[DOI]
- Constantinou A, "The effect of the gene for polledness on conception rate and litter size in the Damascus goat", Ann Genet Sel Anim, 13, pp.111~ (1981)[DOI]
- Al-Ramamneh DS, "The ability of White Leghorn hens with trimmed comb and wattles to thermoregulate", Poult Sci, 95, pp.1726~1735 (2016)[DOI]
- Wright D, "Copy number variation in intron 1 of SOX5 causes the Pea-comb phenotype in chickens", PloS Genet, 5, pp.e1000512~ (2009)[DOI]
- Imsland F, "The Rose-comb mutation in chickens constitutes a structural rearrangement causing both altered comb morphology and defective sperm motility", PloS Genet, 8, pp.e1002775~ (2012)[DOI]
- Zhu H, "Relationship between SNPs of POU1F1 gene and litter size and growth traits in Shaanbei White cashmere goats", Animals, 9, pp.114~ (2019)[DOI]
- Wang K, "A novel SNP in EIF2AK4 gene is associated with thermal tolerance traits in Chinese cattle", Animals, 9, pp.375~ (2019)[DOI]
- Mentuccia D, "Association between a novel variant of the human type 2 deiodinase gene Thr92Ala and insulin resistance : evidence of interaction with the Trp64Arg variant of the beta-3-adrenergic receptor", Diabetes, 51, pp.880~883 (2002)[DOI]
- McAninch EA, "A common DIO2polymorphism and alzheimer disease dementia in African and European Americans", J Clin Endocrinol Metab, 103, pp.1818~1826 (2018)[DOI]
- Jin T, "Association between DIO2 polymorphism and the risk of Kashin-Beck disease in the Tibetan population", J Gene Med, 21, pp.e3123~ (2019)[DOI]
- Leiria LB, "The rs225017 polymorphism in the 3'UTR of the human DIO2 gene is associated with increased insulin resistance", PLoS One, 9, pp.e103960~ (2014)[DOI]
- Fallin D, "Genetic analysis of case", control data using estimated haplotype frequencies : application to APOE locus variation and Alzheimer's disease, 2001, pp.11, 143~151
