본문 바로가기
KERD
논문KCI 등재

Fermentation characteristics and microbial community composition of wet brewer’s grains and corn stover mixed silage prepared with cellulase and lactic acid bacteria supplementation

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Objective: The objective of this study was to investigate how cellulase or/and lactic acid bacteria (LAB) affected the fermentation characteristic and microbial community in wet brewer’s grains (WBG) and corn stover (CS) mixed silage.Methods: The WBG was mixed thoroughly with the CS at 7:3 (w/w). Four treatment groups were studied: i) CON, no additives; ii) CEL, added cellulase (120 U/g fresh matter [FM]), iii) LAB, added LAB (2×10<sup>6</sup> cfu/g FM), and iv) CLA, added cellulase (120 U/g FM) and LAB (2×10<sup>6</sup> cfu/g FM).Results: All additive-treated groups showed higher fermentation quality over the 30 d ensiling period. As these groups exhibited higher (p<0.05) LAB counts and lactic acid (LA) content, along with lower pH value and ammonia-nitrogen (NH<sub>3</sub>-N) content than the control. Specifically, cellulase-treated groups (CEL and CLA) showed lower (p<0.05) neutral detergent fiber and acid detergent fiber contents than other groups. All additives increased the abundance of beneficial bacteria (Firmicutes, <i>Lactiplantibacillus</i>, and <i>Limosilactobacillus</i>) while they decreased abundance of Proteobacteria and microbial diversity as well.Conclusion: The combined application of cellulase and LAB could effectively improve the fermentation quality and microbial community of the WBG and CS mixed silage.

Abstract

Objective: The objective of this study was to investigate how cellulase or/and lactic acid bacteria (LAB) affected the fermentation characteristic and microbial community in wet brewer’s grains (WBG) and corn stover (CS) mixed silage. Methods: The WBG was mixed thoroughly with the CS at 7:3 (w/w). Four treatment groups were studied: i) CON, no additives; ii) CEL, added cellulase (120 U/g fresh matter [FM]), iii) LAB, added LAB (2×106 cfu/g FM), and iv) CLA, added cellulase (120 U/g FM) and LAB (2×106 cfu/g FM). Results: All additive-treated groups showed higher fermentation quality over the 30 d ensiling period. As these groups exhibited higher (p<0.05) LAB counts and lactic acid (LA) content, along with lower pH value and ammonia-nitrogen (NH3-N) content than the control. Specifically, cellulase-treated groups (CEL and CLA) showed lower (p<0.05) neutral detergent fiber and acid detergent fiber contents than other groups. All additives increased the abundance of beneficial bacteria (Firmicutes, Lactiplantibacillus, and Limosilactobacillus) while they decreased abundance of Proteobacteria and microbial diversity as well. Conclusion: The combined application of cellulase and LAB could effectively improve the fermentation quality and microbial community of the WBG and CS mixed silage.

참고문헌 (30)

  1. Wang B, "Effects of storage duration and temperature on the chemical composition, microorganism density, and in vitro rumen fermentation of wet brewers grains", Asian-Australas J Anim Sci, 27, pp.832~840 (2014)[DOI]
  2. Yan Y, "Microbial community and fermentation characteristic of Italian ryegrass silage prepared with corn stover and lactic acid bacteria", Bioresour Technol, 279, pp.166~173 (2019)[DOI]
  3. Zhao C, "Cellulase interacts with lactic acid bacteria to affect fermentation quality, microbial community, and ruminal degradability in mixed silage of soybean residue and corn stover", Animals(Basel), 11, pp.334~ (2021)[DOI]
  4. Zhao S, "Steam explosion enhances digestibility and fermentation of corn stover by facilitating ruminal microbial colonization", Bioresour Technol, 253, pp.244~251 (2018)[DOI]
  5. Ni K, "Assessing the fermentation quality and microbial community of the mixed silage of forage soybean with crop corn or sorghum", Bioresour Technol, 265, pp.563~567 (2018)[DOI]
  6. He L, "Effect of applying lactic acid bacteria and cellulase on the fermentation quality, nutritive value, tannins profile and in vitro digestibility of Neolamarckia cadamba leaves silage", J Anim Physiol Anim Nutr(Berl), 102, pp.1429~1436 (2018)[DOI]
  7. Zhao G, "Effects of applying cellulase and starch on the fermentation characteristics and microbial communities of Napier grass(Pennisetum purpureum Schum. )silage", J Anim Sci Technol, 63, pp.1301~1313 (2021)[DOI]
  8. AOAC International, "Official methods of analysis" (2012), AOAC Int
  9. Van Soest PJ, "Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition", J Dairy Sci, 74, pp.3583~3597 (1991)[DOI]
  10. Yemm EW, "The estimation of carbohydrates in plant extracts by anthrone", Biochem J, 57, pp.508~514 (1954)[DOI]
  11. Broderick GA, "Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media", J Dairy Sci, 63, pp.64~75 (1980)[DOI]
  12. Olsen RA, "Viability of soil bacteria : Optimization of plate-counting technique and comparison between total counts and plate counts within different size groups", Microb Ecol, 13, pp.59~74 (1987)[DOI]
  13. Guan H, "Microbial communities and natural fermentation of corn silages prepared with farm bunkersilo in Southwest China", Bioresour Technol, 265, pp.282~290 (2018)[DOI]
  14. Hu W, "The effect of Lactobacillus buchneri 40788 or Lactobacillus plantarum MTD-1 on the fermentation and aerobic stability of corn silages ensiled at two dry matter contents", J Dairy Sci, 92, pp.3907~3914 (2009)[DOI]
  15. Mu L, "Lactobacillus plantarum and molasses alter dynamic chemical composition, microbial community, and aerobic stability of mixed(amaranth and rice straw)silage", J Sci Food Agric, 101, pp.5225~5235 (2021)[DOI]
  16. Zhang JG, "Relationships between the addition rates of cellulase or glucose and silage fermentation at different temperatures", Anim Sci J, 81, pp.325~330 (2010)[DOI]
  17. He L, "Ensiling characteristics, proteolysis and bacterial community of high-moisture corn stalk and stylo silage prepared with Bauhinia variegate flower", Bioresour Technol, 296, pp.122336~ (2020)[DOI]
  18. Guo G, "Effects of fibrolytic enzymes, molasses and lactic acid bacteria on fermentation quality of mixed silage of corn and hulless–barely straw in the Tibetan Plateau", Grassl Sci, 60, pp.240~246 (2014)[DOI]
  19. Arriola KG, "Effect of microbial inoculants on the quality and aerobic stability of bermudagrass round-bale haylage", J Dairy Sci, 98, pp.478~485 (2015)[DOI]
  20. Mu L, "Cellulase interacts with Lactobacillus plantarum to affect chemical composition, bacterial communities, and aerobic stability in mixed silage of high-moisture amaranth and rice straw", Bioresour Technol, 315, pp.123772~ (2020)[DOI]
  21. Ebrahimi M, "The effects of adding lactic acid bacteria and cellulase in oil palm(Elais Guineensis Jacq. )frond silages on fermentation quality, chemical composition and in vitro digestibility", Ital J Anim Sci, 13, pp.3358~ (2014)[DOI]
  22. Hassanat F, "Effects of inoculation on ensiling characteristics, chemical composition and aerobic stability of regular and brown midrib millet silages", Anim Feed Sci Technol, 139, pp.125~140 (2007)[DOI]
  23. Li J, "Characterization of Enterococcus faecalis JF85 and Enterococcus faecium Y83 isolated from Tibetan yak(Bos grunniens)for ensiling Pennisetum sinese", Bioresour Technol, 257, pp.76~83 (2018)[DOI]
  24. Kung L Jr, "Silage review : Interpretation of chemical, microbial, and organoleptic components of silages", J Dairy Sci, 101, pp.4020~4033 (2018)[DOI]
  25. Ogunade IM, "Fate of Escherichia coli O157 : H7 and bacterial diversity in corn silage contaminated with the pathogen and treated with chemical or microbial additives", J Dairy Sci, 100, pp.1780~1794 (2017)[DOI]
  26. Romero JJ, "Laboratory silo type and inoculation effects on nutritional composition, fermentation, and bacterial and fungal communities of oat silage", J Dairy Sci, 100, pp.1812~1828 (2017)[DOI]
  27. Yuan XJ, "Microbial community dynamics and their contributions to organic acid production during the early stage of the ensiling of Napier grass(Pennisetum purpureum)", Grass Forage Sci, 75, pp.37~44 (2020)[DOI]
  28. Cheng Q, "Effects of LAB inoculants on the fermentation quality, chemical composition, and bacterial community of oat silage on the QinghaiTibetan plateau", Microorganisms, 10, pp.787~ (2022)[DOI]
  29. Xu D, "Modulation of Metabolome and Bacterial Community in Whole Crop Corn Silage by Inoculating Homofermentative Lactobacillus plantarum and Heterofermentative Lactobacillus buchneri", Front Microbiol, 9, pp.3299~ (2019)[DOI]
  30. Wang C, "Fermentation quality and microbial community of alfalfa and stylo silage mixed with Moringa oleifera leaves", Bioresour Technol, 284, pp.240~247 (2019)[DOI]

관련 논문AI추천

Effects of applying cellulase and starch on the fermentation characteristics and microbial communities of Napier grass (Pennisetum purpureum Schum.) silageGuoqiang Zhao, Hao Wu, Jiajun He, Li Li, Xiangxue Xie, Xinjian Yang, Zhichao Hu한국축산학회 · 한국축산학회지2021 · 제63권 제6호Chemical composition and in vitro digestibility of corn stover during field exposure and the fermentation characteristics of silage prepared with microbial additivesChang-Hai Zhou, Hong Yu Tang, Jiabao Zhang, Jun Lei Gao, Peng Wang, Ping Li, Yimin Cai아세아·태평양축산학회 · Animal Bioscience2019 · 제32권 제12호Lactic acid bacteria strains selected from fermented total mixed rations improve ensiling and in vitro rumen fermentation characteristics of corn stover silageChen Hongwei, Hong Qihua, Huang Kailang, Liu Yalu, Wang Jiakun, Yang Bin아세아·태평양축산학회 · Animal Bioscience2022 · 제35권 제9호Effects of microbial enzymes on starch and hemicellulose degradation in total mixed ration silagesC.C. Xu, Dongze Niu, Huili Wang, Mingli Zheng, Sasa Zuo, Tingting Ning아세아·태평양축산학회 · Animal Bioscience2017 · 제30권 제2호Effects of Microbial Additives on Chemical Composition and Fermentation Characteristics of Barley Silage김동현, 김삼철, H.J. Lee, S.B. Kim, S.M.Amanullah, Y.H. Joo아세아·태평양축산학회 · Animal Bioscience2014 · 제27권 제4호Effect of Microbial and Chemical Combo Additives on Nutritive Value and Fermentation Characteristic of Whole Crop Barley Silage김동현, 김삼철, 이혁준, 주영호, Sardar M. Amanullah아세아·태평양축산학회 · Animal Bioscience2015 · 제28권 제9호

이 간행물의 다른 논문 (14건)