Effects of applying cellulase and starch on the fermentation characteristics and microbial communities of Napier grass (Pennisetum purpureum Schum.) silage
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This study investigated the effects of applying cellulase and starch on the fermentation characteristics and microbial communities of Napier grass silage after ensiling for 30 d. Three groups were studied: No additives (control); added cellulase (Group 1); and added cellulase and starch (Group 2). The results showed that the addition of cellulase and starch decreased the crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and pH significantly (p < 0.05) and increased water-soluble carbohydrate (WSC) content (p < 0.05). The addition of additives in two treated groups exerted a positive effect on the lactic acid (LA) content, lactic acid bacteria (LAB) population, and lactic acid / acetic acid (LA/AA) ratio, even the changes were not significant (p > 0.05). Calculation of Flieg’s scores indicated that cellulase application increased silage quality to some extent, while the application of cellulase and starch together significantly improved fermentation (p < 0.05). Compared with the control, both additive groups showed increased microbial diversity after ensiling with an abundance of favorable bacteria including Firmicutes and Weissella, and the bacteria including Proteobacteria, Bacteroidetes, Acinetobacter increased as well. For alpha diversity analysis, the combined application of cellulase and starch in Group 2 gave significant increases in all indices (p < 0.05). The study demonstrated that the application of cellulase and starch can increase the quality of Napier grass preserved as silage
Abstract
This study investigated the effects of applying cellulase and starch on the fermentation characteristics and microbial communities of Napier grass silage after ensiling for 30 d. Three groups were studied: No additives (control); added cellulase (Group 1); and added cellulase and starch (Group 2). The results showed that the addition of cellulase and starch decreased the crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and pH significantly (p < 0.05) and increased water-soluble carbohydrate (WSC) content (p < 0.05). The addition of additives in two treated groups exerted a positive effect on the lactic acid (LA) content, lactic acid bacteria (LAB) population, and lactic acid / acetic acid (LA/AA) ratio, even the changes were not significant (p > 0.05). Calculation of Flieg’s scores indicated that cellulase application increased silage quality to some extent, while the application of cellulase and starch together significantly improved fermentation (p < 0.05). Compared with the control, both additive groups showed increased microbial diversity after ensiling with an abundance of favorable bacteria including Firmicutes and Weissella, and the bacteria including Proteobacteria, Bacteroidetes, Acinetobacter increased as well. For alpha diversity analysis, the combined application of cellulase and starch in Group 2 gave significant increases in all indices (p < 0.05). The study demonstrated that the application of cellulase and starch can increase the quality of Napier grass preserved as silage
참고문헌 (53)
- Niaz Ali, "Using a high-throughput sequencing technology to evaluate the various forage source epiphytic microbiota and their effect on fermentation quality and microbial diversity of Napier grass", Archives of Microbiology, 203(8), pp.4879~4890 (2021), Springer Science and Business Media LLC[DOI]
- Cai Yimin, "Influence of microbial additive on microbial populations, ensiling characteristics, and spoilage loss of delayed sealing silage of Napier grass", Animal Bioscience, 33(7), pp.1103~1112 (2020), 아세아·태평양축산학회[DOI]
- Junfeng Li, "Characterization of Enterococcus faecalis JF85 and Enterococcus faecium Y83 isolated from Tibetan yak (Bos grunniens) for ensiling Pennisetum sinese", Bioresource Technology, 257, pp.76~83 (2018), Elsevier BV[DOI]
- Mcdonald P, "The biochemistry of silage" (1991), Chalcombe
- M.R. Dehghani, "Effect of enzyme addition to forage at ensiling on silage chemical composition and NDF degradation characteristics", Livestock Science, 150(1-3), pp.51~58 (2012), Elsevier BV[DOI]
- Seare T. Desta, "Ensiling characteristics, structural and nonstructural carbohydrate composition and enzymatic digestibility of Napier grass ensiled with additives", Bioresource Technology, 221, pp.447~454 (2016), Elsevier BV[DOI]
- Ali Gulfam, "Characteristics of lactic acid bacteria isolates and their effect on the fermentation quality of Napier grass silage at three high temperatures", Journal of the Science of Food and Agriculture, 97(6), pp.1931~1938 (2017), Wiley[DOI]
- Waroon Khota, "Comparative analysis of silage fermentation and in vitro digestibility of tropical grass prepared with Acremonium and Tricoderma species producing cellulases", Animal Bioscience, 31(12), pp.1913~1922 (2018), 아세아·태평양축산학회[DOI]
- Niaz Ali, "Microbial diversity and fermentation profile of red clover silage inoculated with reconstituted indigenous and exogenous epiphytic microbiota", Bioresource Technology, 314, pp.123606~ (2020), Elsevier BV[DOI]
- Miaoyin Dong, "Effects of microbial inoculants on the fermentation characteristics and microbial communities of sweet sorghum bagasse silage", Scientific Reports, 10(1), pp.837~ (2020), Springer Science and Business Media LLC[DOI]
- Association of Official Analytical Chemists, "Official methods of analysis" (1990), AOAC
- P.J. Van Soest, "Methods for Dietary Fiber, Neutral Detergent Fiber, and Nonstarch Polysaccharides in Relation to Animal Nutrition", Journal of Dairy Science, 74(10), pp.3583~3597 (1991), American Dairy Science Association[DOI]
- E. W. Yemm, "The estimation of carbohydrates in plant extracts by anthrone", Biochemical Journal, 57(3), pp.508~514 (1954), Portland Press Ltd.[DOI]
- G.A. Broderick, "Automated Simultaneous Determination of Ammonia and Total Amino Acids in Ruminal Fluid and In Vitro Media", Journal of Dairy Science, 63(1), pp.64~75 (1980), American Dairy Science Association[DOI]
- X. Q. Zhang, "Silage quality and preservation ofUrtica cannabinaensiled alone and with additive treatment", Grass and Forage Science, 69(3), pp.405~414 (2014), Wiley[DOI]
- Guo Qiang Zhao, "Effect of harvest dates on β-carotene content and forage quality of rye (Secale cereale L.) silage and hay", 한국축산학회지, 63(2), pp.354~366 (2021), 한국축산학회[DOI]
- Madigan MT, "Brock biology of microorganisms" (2012), Pearson
- T. Magoc, "FLASH: fast length adjustment of short reads to improve genome assemblies", Bioinformatics, 27(21), pp.2957~2963 (2011), Oxford University Press (OUP)[DOI]
- J Gregory Caporaso, "QIIME allows analysis of high-throughput community sequencing data", Nature Methods, 7(5), pp.335~336 (2010), Springer Science and Business Media LLC[DOI]
- A. R. Kemble, "Studies on the nitrogen metabolism of the ensilage process", Journal of the Science of Food and Agriculture, 7(2), pp.125~130 (1956), Wiley[DOI]
- Mitsuaki Ohshima, "A review of the changes in nitrogenous compounds of herbage during ensilage", Journal of the Science of Food and Agriculture, 29(6), pp.497~505 (1978), Wiley[DOI]
- B. A. Jones, "Characterization of Proteolysis in Alfalfa and Red Clover", Crop Science, 35(2), pp.537~541 (1995), Wiley[DOI]
- R. E. PITT, "A quantitative model of the ensilage process in lactate silages", Grass and Forage Science, 40(3), pp.279~303 (1985), Wiley[DOI]
- Maja Fijałkowska, "Changes of Nitrogen Compounds During Ensiling of High Protein Herbages – A Review", Annals of Animal Science, 15(2), pp.289~305 (2015), Walter de Gruyter GmbH[DOI]
- Ya Tao, "Comparative analysis of ensiling characteristics and protein degradation of alfalfa silage prepared with corn or sweet sorghum in semiarid region of Inner Mongolia", Animal Science Journal, 91(1), pp.e13321~ (2020), Wiley[DOI]
- Liwen He, "Effect of applying lactic acid bacteria and cellulase on the fermentation quality, nutritive value, tannins profile and in vitro digestibility ofNeolamarckia cadambaleaves silage", Journal of Animal Physiology and Animal Nutrition, 102(6), pp.1429~1436 (2018), Wiley[DOI]
- Rina Su, "Effects of ferulic acid esterase-producingLactobacillus fermentumand cellulase additives on the fermentation quality and microbial community of alfalfa silage", PeerJ, 7, pp.e7712~ (2019), PeerJ[DOI]
- Siran Wang, "Improvement of fermentation profile and structural carbohydrate compositions in mixed silages ensiled with fibrolytic enzymes, molasses and Lactobacillus plantarum MTD-1", Italian Journal of Animal Science, 18(1), pp.328~335 (2019), Informa UK Limited[DOI]
- Mao Li, "Silage fermentation and ruminal degradation of stylo prepared with lactic acid bacteria and cellulase", Animal Science Journal, 88(10), pp.1531~1537 (2017), Wiley[DOI]
- D. I. H. JONES, "The effect of cereal incorporation on the fermentation of spring-and autumn-cut silages in laboratory silos", Grass and Forage Science, 43(2), pp.167~172 (1988), Wiley[DOI]
- N. Karimi Rahjerdi, "Chemical composition, fermentation characteristics, digestibility, and degradability of silages from two amaranth varieties (Kharkovskiy and Sem), corn, and an amaranth–corn combination", Journal of Animal Science, 93(12), pp.5781~5790 (2015), Oxford University Press (OUP)[DOI]
- Jipeng Tian, "Effects of lactic acid bacteria inoculants and cellulase on fermentation quality and in vitro digestibility of Leymus chinensis silage", Grassland Science, 60(4), pp.199~205 (2014), Wiley[DOI]
- J. G. Kim, "Effects of Maturity at Harvest and Wilting Days on Quality of Round Baled Rye Silage", Asian-Australasian Journal of Animal Sciences, 14(9), pp.1233~1237 (2001), Asian Australasian Association of Animal Production Societies[DOI]
- Bolsen KK, "Forages, volume II: the science of grassland agriculture", pp.163~176 (1995), Iowa State University Press
- Wieringa GW, "The influence of nitrate on silage fermentation", Proceedings of the 4th International Grassland Congress, pp.537~540 (1966)
- B. A. JONES, "Influence of bacterial inoculant and substrate addition to lucerne ensiled at different dry matter contents", Grass and Forage Science, 47(1), pp.19~27 (1992), Wiley[DOI]
- N. Gollop, "Antibacterial activity of lactic acid bacteria included in inoculants for silage and in silages treated with these inoculants", Journal of Applied Microbiology, 98(3), pp.662~666 (2005), Wiley[DOI]
- Yi Wang, "Bacterial diversity and fermentation quality of Moringa oleifera leaves silage prepared with lactic acid bacteria inoculants and stored at different temperatures", Bioresource Technology, 284, pp.349~358 (2019), Elsevier BV[DOI]
- Liangyin Chen, "Effect of a low temperature tolerant lactic acid bacteria inoculant on the fermentation quality and bacterial community of oat round bale silage", Animal Feed Science and Technology, 269, pp.114669~ (2020), Elsevier BV[DOI]
- Siran Wang, "Sequencing and microbiota transplantation to determine the role of microbiota on the fermentation type of oat silage", Bioresource Technology, 309, pp.123371~ (2020), Elsevier BV[DOI]
- J.J. Romero, "Laboratory silo type and inoculation effects on nutritional composition, fermentation, and bacterial and fungal communities of oat silage", Journal of Dairy Science, 100(3), pp.1812~1828 (2017), American Dairy Science Association[DOI]
- Kuikui Ni, "Assessing the fermentation quality and microbial community of the mixed silage of forage soybean with crop corn or sorghum", Bioresource Technology, 265, pp.563~567 (2018), Elsevier BV[DOI]
- Jinsong Yang, "Characteristics of lactic acid bacteria isolates and their effect on silage fermentation of fruit residues", Journal of Dairy Science, 99(7), pp.5325~5334 (2016), American Dairy Science Association[DOI]
- Kuikui Ni, "Natural Lactic Acid Bacteria Population and Silage Fermentation of Whole-crop Wheat", Animal Bioscience, 28(8), pp.1123~1132 (2015), 아세아·태평양축산학회[DOI]
- Shanwei Xu, "Impact of Saccharomyces cerevisiae and Lactobacillus buchneri on microbial communities during ensiling and aerobic spoilage of corn silage1", Journal of Animal Science, 97(3), pp.1273~1285 (2019), Oxford University Press (OUP)[DOI]
- Yanhong Yan, "Microbial community and fermentation characteristic of Italian ryegrass silage prepared with corn stover and lactic acid bacteria", Bioresource Technology, 279, pp.166~173 (2019), Elsevier BV[DOI]
- Lili Yang, "Dynamics of microbial community and fermentation quality during ensiling of sterile and nonsterile alfalfa with or without Lactobacillus plantarum inoculant", Bioresource Technology, 275, pp.280~287 (2019), Elsevier BV[DOI]
- Yi Wang, "Effects of Wilting and Lactobacillus plantarum Addition on the Fermentation Quality and Microbial Community of Moringa oleifera Leaf Silage", Frontiers in Microbiology, 9, pp.1817~ (2018), Frontiers Media SA[DOI]
- Liwen He, "Dynamics of proteolysis, protease activity and bacterial community of Neolamarckia cadamba leaves silage and the effects of formic acid and Lactobacillus farciminis", Bioresource Technology, 294, pp.122127~ (2019), Elsevier BV[DOI]
- I.M. Ogunade, "Bacterial diversity and composition of alfalfa silage as analyzed by Illumina MiSeq sequencing: Effects of Escherichia coli O157:H7 and silage additives", Journal of Dairy Science, 101(3), pp.2048~2059 (2018), American Dairy Science Association[DOI]
- Celia Méndez-GarcÃa, "Microbial diversity and metabolic networks in acid mine drainage habitats", Frontiers in Microbiology, 6, pp.475~ (2015), Frontiers Media SA[DOI]
- M.L. Zheng, "Dynamics of microbial community during ensiling direct-cut alfalfa with and without LAB inoculant and sugar", Journal of Applied Microbiology, 122(6), pp.1456~1470 (2017), Wiley[DOI]
- S. Parvin, "Effects of inoculation with lactic acid bacteria on the bacterial communities of Italian ryegrass, whole crop maize, guinea grass and rhodes grass silages", Animal Feed Science and Technology, 160(3-4), pp.160~166 (2010), Elsevier BV[DOI]
