Using Azolla (Azolla microphylla) leaf meal and phytonutrient powder on rumen fermentation efficiency and nutrient degradability using in vitro technique
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objective: This work was to investigate the effect of using <i>Azolla</i> (<i>Azolla microphylla</i>) leaf meal and phytonutrient powder on rumen fermentation efficiency and nutrient degradability using <i>in vitro</i> technique.Methods: All respective treatments were imposed in a 2×4×2 Factorial arrangements according to a completely randomized design. The first factor was two ratios of roughage to concentrate (R:C at 60:40, and 40:60), the second factor was <i>Azolla</i> (<i>Azolla microphylla</i>) powder (AMP) supplementation levels (0%, 3%, 6%, and 9% of the total substrate) and the third factor was turmeric (<i>Curcuma longa</i>) powder (TUP) supplementation levels (0% and 2% of the total substrate).Results: Cumulative gas production at 96 h, was affected by R:C and numerically increased by AMP and TUP supplementation (p<0.05). Gas production kinetics increased with the increasing ratio of concentrate and AMP supplementation whereas TUP supplement reduced gas production. <i>In vitro</i> dry matter degradability was remarkably increased (p<0.05) by the R:C ratio, AMP and TUP supplementation. However, increasing R:C ratio, AMP and TUP supplementation resulted in the concentration of propionate (C<sub>3</sub>) significantly increasing (p<0.05). Acetate (C<sub>2</sub>), C<sub>2</sub>:C<sub>3</sub> ratio, and protozoal population were improved (p<0.05), while the methane production decreased.Conclusion: Under this study, the results were obtained under the supplementation level of 9% AMP and 2% TUP of total substrate, hence, the combined use is potentially beneficial. These results revealed a potential use of AMP and TUP as a supplement to improve rumen fermentation for ruminant feeding. Nevertheless, in vivo feeding trials should be further investigated using AMP and TUP as a source of protein and phytonutrient.
Abstract
Objective: This work was to investigate the effect of using Azolla (Azolla microphylla) leaf meal and phytonutrient powder on rumen fermentation efficiency and nutrient degradability using in vitro technique. Methods: All respective treatments were imposed in a 2×4×2 Factorial arrangements according to a completely randomized design. The first factor was two ratios of roughage to concentrate (R:C at 60:40, and 40:60), the second factor was Azolla (Azolla microphylla) powder (AMP) supplementation levels (0%, 3%, 6%, and 9% of the total substrate) and the third factor was turmeric (Curcuma longa) powder (TUP) supplementation levels (0% and 2% of the total substrate). Results: Cumulative gas production at 96 h, was affected by R:C and numerically increased by AMP and TUP supplementation (p<0.05). Gas production kinetics increased with the increasing ratio of concentrate and AMP supplementation whereas TUP supplement reduced gas production. In vitro dry matter degradability was remarkably increased (p<0.05) by the R:C ratio, AMP and TUP supplementation. However, increasing R:C ratio, AMP and TUP supplementation resulted in the concentration of propionate (C3) significantly increasing (p<0.05). Acetate (C2), C2:C3 ratio, and protozoal population were improved (p<0.05), while the methane production decreased. Conclusion: Under this study, the results were obtained under the supplementation level of 9% AMP and 2% TUP of total substrate, hence, the combined use is potentially beneficial. These results revealed a potential use of AMP and TUP as a supplement to improve rumen fermentation for ruminant feeding. Nevertheless, in vivo feeding trials should be further investigated using AMP and TUP as a source of protein and phytonutrient.