Essential oil mixture on rumen fermentation and microbial community – an in vitro study
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Objective: The objective of this study was to investigate the effects of essential oil mixture (EOM) supplementation on rumen fermentation characteristics and microbial changes in an in vitro. Methods: Three experimental treatments were used: control (CON, no additive), EOM 0.1 (supplementation of 1 g EOM/kg of substrate), and EOM 0.2 (supplementation of 2 g EOM/kg of substrate). An in vitro fermentation experiment was carried out using strained rumen fluid for 12 and 24 h incubation periods. At each time point, in vitro dry matter digestibility (IVDMD), neutral detergent fiber digestibility (IVNDFD), pH, ammonia nitrogen (NH3-N), and volatile fatty acid (VFA) concentrations, and relative microbial diversity were estimated. Results: After 24 h incubation, treatments involving EOM supplementation led to significantly higher IVDMD (treatments and quadratic effect; p = 0.019 and 0.008) and IVNDFD (linear effect; p = 0.068) than did the CON treatment. The EOM 0.2 supplementation group had the highest NH3-N concentration (treatments; p = 0.032). Both EOM supplementations did not affect total VFA concentration and the proportion of individual VFAs; however, total VFA tended to increase in EOM supplementation groups, after 12 h incubation (linear; p = 0.071). Relative protozoa abundance significantly increased following EOM supplementation (treatments, p<0.001). Selenomonas ruminantium and Ruminococcus albus (treatments; p<0.001 and p = 0.005), abundance was higher in the EOM 0.1 treatment group than in CON. The abundance of Butyrivibrio fibrisolvens, fungi and Ruminococcus flavefaciens (treatments; p< 0.001, p<0.001, and p = 0.005) was higher following EOM 0.2 treatment. Conclusion: The addition of newly developed EOM increased IVDMD, IVNDFD, and tended to increase total VFA indicating that it may be used as a feed additive to improve rumen fermentation by modulating rumen microbial communities. Further studies would be required to investigate the detailed metabolic mechanism underlying the effects of EOM supplementation.
Abstract
Objective: The objective of this study was to investigate the effects of essential oil mixture (EOM) supplementation on rumen fermentation characteristics and microbial changes in an in vitro. Methods: Three experimental treatments were used: control (CON, no additive), EOM 0.1 (supplementation of 1 g EOM/kg of substrate), and EOM 0.2 (supplementation of 2 g EOM/kg of substrate). An in vitro fermentation experiment was carried out using strained rumen fluid for 12 and 24 h incubation periods. At each time point, in vitro dry matter digestibility (IVDMD), neutral detergent fiber digestibility (IVNDFD), pH, ammonia nitrogen (NH3-N), and volatile fatty acid (VFA) concentrations, and relative microbial diversity were estimated. Results: After 24 h incubation, treatments involving EOM supplementation led to significantly higher IVDMD (treatments and quadratic effect; p = 0.019 and 0.008) and IVNDFD (linear effect; p = 0.068) than did the CON treatment. The EOM 0.2 supplementation group had the highest NH3-N concentration (treatments; p = 0.032). Both EOM supplementations did not affect total VFA concentration and the proportion of individual VFAs; however, total VFA tended to increase in EOM supplementation groups, after 12 h incubation (linear; p = 0.071). Relative protozoa abundance significantly increased following EOM supplementation (treatments, p<0.001). Selenomonas ruminantium and Ruminococcus albus (treatments; p<0.001 and p = 0.005), abundance was higher in the EOM 0.1 treatment group than in CON. The abundance of Butyrivibrio fibrisolvens, fungi and Ruminococcus flavefaciens (treatments; p< 0.001, p<0.001, and p = 0.005) was higher following EOM 0.2 treatment. Conclusion: The addition of newly developed EOM increased IVDMD, IVNDFD, and tended to increase total VFA indicating that it may be used as a feed additive to improve rumen fermentation by modulating rumen microbial communities. Further studies would be required to investigate the detailed metabolic mechanism underlying the effects of EOM supplementation.
참고문헌 (30)
- Russell JB, "Ionophore resistance of ruminal bacteria and its potential impact on human health", FEMS Microbiol Rev, 27, pp.65~74 (2003)
- Benchaar C, "Effects of essential oils and their components on in vitro rumen microbial fermentation", Can J Anim Sci, 87, pp.413~419 (2007)
- Dorman HJ, "Antimicrobial agents from plants : antibacterial activity of plant volatile oils", J Appl Microbiol, 88, pp.308~316 (2000)
- Kim J, "Antibacterial activity of some essential oil components against five foodborne pathogens", J Agric Food Chem, 43, pp.2839~2845 (1995)
- Macheboeuf D, "Dose–response effects of essential oils on in vitro fermentation activity of the rumen microbial population", Anim Feed Sci Technol, 145, pp.335~350 (2008)
- Busquet M, "Plant extracts affect in vitro rumen microbial fermentation", J Dairy Sci, 89, pp.761~771 (2006)
- Castillejos L, "Effect of essential oil active compounds on rumen microbial fermentation and nutrient flow in in vitro systems", J Dairy Sci, 89, pp.2649~2658 (2006)
- Busquet M, "Effects of cinnamaldehyde and garlic oil on rumen microbial fermentation in a dual flow continuous culture", J Dairy Sci, 88, pp.2508~2516 (2005)
- Castillejos L, "Effects of a specific blend of essential oil compounds and the type of diet on rumen microbial fermentation and nutrient flow from a continuous culture system", Anim Feed Sci Technol, 119, pp.29~41 (2005)
- Fraser GR, "Assessment of the effects of cinnamon leaf oil on rumen microbial fermentation using two continuous culture systems", J Dairy Sci, 90, pp.2315~2328 (2007)
- Newbold CJ, "Effects of a specific blend of essential oil compounds on rumen fermentation", Anim Feed Sci Technol, 114, pp.105~112 (2004)
- Tokarev V, "Novatan-50 use in lactating cow feed", Russ Agric Sci, 39, pp.167~169 (2013)
- Forage fiber analysis (apparatus reagents, procedures, and some applications), "Goering H", U.S. Government Printing Office, ~ (1970)
- Theodorou MK, "A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds", Anim Feed Sci Technol, 48, pp.185~197 (1994)
- Pell AN, "Computerized monitoring of gas production to measure forage digestion in vitro", J Dairy Sci, 76, pp.1063~1073 (1993)
- Denman SE, "Development of a real‐time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen", FEMS Microbiol Ecol, 58, pp.572~582 (2006)
- Khafipour E, "Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis", Appl Environ Microbiol, 75, pp.7115~7124 (2009)
- Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method", Methods, 25, pp.402~408 (2001)
- Jinshun Zhan, "Effects of alfalfa flavonoids on the production performance, immune system, and ruminal fermentation of dairy cows", Animal Bioscience, 30(10), pp.1416~1424 (2017), 아세아·태평양축산학회[DOI]
- Wendakoon CN, "Inhibition of amino acid decarboxylase activity of Enterobacter aerogenes by active components in spices", J Food Prot, 58, pp.280~283 (1995)
- Castillejos L, "Effects of dose and adaptation time of a specific blend of essential oil compounds on rumen fermentation", Anim Feed Sci Technol, 132, pp.186~201 (2007)
- Newbold CJ, "The role of ciliate protozoa in the rumen", Front Microbiol, 6, pp.1313~ (2015)
- Satoshi Koike, "Fibrolytic Rumen Bacteria: Their Ecology and Functions", Animal Bioscience, 22(1), pp.131~138 (2009), 아세아·태평양축산학회
- Varel VH, "Ruminal cellulolytic bacteria and protozoa from bison, cattle-bison hybrids, and cattle fed three alfalfa-corn diets", Appl Environ Microbiol, 55, pp.148~153 (1989)
- Wubah DA, "Biology, fiber-degradation, and enzymology of anaerobic zoosporic fungi", Crit Rev Microbiol, 19, pp.99~115 (1993)
- Yang WZ, "Effects of garlic and juniper berry essential oils on ruminal fermentation and on the site and extent of digestion in lactating cows", J Dairy Sci, 90, pp.5671~5681 (2007)
- Matloup O, "Performance of lactating Friesian cows fed a diet supplemented with coriander oil : feed intake, nutrient digestibility, ruminal fermentation, blood chemistry, and milk production", Anim Feed Sci Technol, 226, pp.88~97 (2017)
- Wang R-F, "PCR detection of Ruminococcus spp. in human and animal faecal samples", Mol Cell Probes, 11, pp.259~265 (1997)
- Sylvester JT, "Development of an assay to quantify rumen ciliate protozoal biomass in cows using real-time PCR", J Nutr, 134, pp.3378~3384 (2004)
- Stevenson DM, "Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR", Appl Microbiol Biotechnol, 75, pp.165~174 (2007)