모체운동이 새끼 쥐의 텔로미어 길이와 텔로미어 조절 단백질 발현에 미치는 영향
Effect of Maternal Exercise on Telomere Length and Telomere-Regulating Protein of Offspring in Rat
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PURPOSE: This study was performed to investigate the effect of maternal exercise on cardiac and skeletal muscle telomere length and telomere repeat binding factor 2 (TRF2) in rat offspring. METHODS: Sprague-Dawley rats (10 wk, N=21) were randomly assigned into control (CON), exercise during pregnancy (ExD), and exercise before and during pregnancy (ExBD) groups. Maternal training was performed on a rodent treadmill for 1 hour at 60-75% VO2max for 3 weeks (5 d/w) before and during pregnancy respectively. The offspring were sacrificed at birth and telomere length and TRF2 expression were measured with cardiac and skeletal muscle samples. RESULTS: CON group had significantly longer telomere length (p=.011, p=.049) and higher TRF2 expression (p=.011, p=.002) than other two groups in skeletal muscle of offspring. In the cardiac muscle, CON group showed longer telomere length compared with ExD group in offspring (p=.001). CONCLUSIONS: Intense maternal exercise before or during pregnancy may negatively affect their new offspring’s cardiac and skeletal muscle telomere length and TRF2 expression.
Abstract
PURPOSE: This study was performed to investigate the effect of maternal exercise on cardiac and skeletal muscle telomere length and telomere repeat binding factor 2 (TRF2) in rat offspring. METHODS: Sprague-Dawley rats (10 wk, N=21) were randomly assigned into control (CON), exercise during pregnancy (ExD), and exercise before and during pregnancy (ExBD) groups. Maternal training was performed on a rodent treadmill for 1 hour at 60-75% VO2max for 3 weeks (5 d/w) before and during pregnancy respectively. The offspring were sacrificed at birth and telomere length and TRF2 expression were measured with cardiac and skeletal muscle samples. RESULTS: CON group had significantly longer telomere length (p=.011, p=.049) and higher TRF2 expression (p=.011, p=.002) than other two groups in skeletal muscle of offspring. In the cardiac muscle, CON group showed longer telomere length compared with ExD group in offspring (p=.001). CONCLUSIONS: Intense maternal exercise before or during pregnancy may negatively affect their new offspring’s cardiac and skeletal muscle telomere length and TRF2 expression.
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