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트레드밀 운동이 streptozotocin에 의해 유발된 당뇨 쥐의 망막 신경세포 사멸에 미치는 영향

Effect of Treadmill Exercise on Apoptosis in the Retinas of Streptozotocin-Induced Diabetic Rats.

정선영|김태운|성윤희|김대영
한국운동생리학회|2012.08|운동과학 (Exercise Science)|제21권 제3호|pp. 289-298|DOI 10.15857/ksep.2012.21.3.289

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키워드

당뇨병Diabetes mellitusDiabetic retinopathyApoptosis세포사멸망막트레드밀 운동Treadmill exercise당뇨성 망막증Retinas

초록

본 연구는 당뇨를 유발한 흰쥐에서 트레드밀 운동이 망막 신경세포의 사멸에 억제 효과가 있는지를 실험 하였다. 본 연구에서 Sprague-Dawley계 흰쥐 28마리를 대조군, 운동군, 당뇨군, 당뇨운동군으로 분류하여 각 군당 7마리씩 배정하였다. 당뇨는 streptozotocin을 복강에 주사하여 유발하였다. 운동군은 분당 8 m의 속도로 하루 30분씩 주 5회, 총 12주 동안 트레드밀 운동을 실시하였다. 본 연구의 결과, 당뇨 유발 흰쥐의 망막에서 세포사멸 인자인 terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)-양성 세포수 그리고 caspase-3, cytochrome c, Bax의 발현이 증가되었으며, 항 세포사멸 인자인 Bcl-2의 발현은 감소되었다. 트레드밀 운동은 TUNEL-양성 세포수 그리고 caspase-3, cytochrome c, Bax의 발현을 감소시켰으며, Bcl-2의 발현은 증가시켰다. 본 실험의 결과, 당뇨에 의한 망막의 세포사멸 증가에 트레드밀 운동이 억제 작용을 나타내었으며, 따라서 트레드밀 운동은 당뇨 환자들에서 후유증을 경감시키는데 효과적인 치료법임을 알 수 있었다.

Abstract

In the present study, we investigated the effect of treadmill exercise on apoptotic neuronal cell death in the retinas of streptozotocin-induced diabetic rats. Twenty-eight male Sprague-Dawley rats were used for this study. The animals were divided into four groups(n = 7 in each group)：(1) control group, (2) exercise group, (3) diabetes-induced group, (4) diabetes-induced and exercise group. Diabetes mellitus(DM) was induced by intraperitoneal injection of streptozotocin. The rats in the exercise groups were forced to run on the treadmill for 30 minutes once a day, five times per a week, during 12 weeks. In this study, a terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) assay and western blot for the expressions of caspase-3, cytochrome c, Bax, and Bcl-2 in the retinas were conducted for the detection of apoptotic retinal cell death. The present results showed that the number of TUNEL-positive cells was increased in the retinas of the diabetic rats, whereas treadmill exercise suppressed this number. The expressions of pro-apoptotic factors caspase-3, cytochrome c, and Bax were enhanced and the expressions of anti-apoptotic factor Bcl-2 was decreased in the retinas of the diabetic rats. In contrast, treadmill exercise suppressed the expressions of caspase-3, cytochrome c, and Bax and increased the expression of Bcl-2. The present study demonstrated that treadmill exercise suppressed diabetes-induced apoptotic neuronal cell death in the retinas. Based on the present results, treadmill exercise may be effective therapeutic strategy for the alleviating complications of diabetes patients.

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수영운동이 당뇨쥐 골격근 조직세포의 GLUTs, MCTs, LDH 및 Caspase-3 발현에 미치는 영향임은미한국운동생리학회 · 운동과학2014 · 제23권 제4호트레드밀 운동이 스트렙토조토신으로 당뇨 유발 후 좌골신경 손상 쥐의 신경재생, 척수운동신경세포, GAP-43와 GLUT-4 단백질 발현에 미치는 영향김종오, 서태범, 윤진환국민체육진흥공단 한국스포츠과학원 · 체육과학연구2006 · 제17권 제3호트레드밀 운동이 streptozotocin로 유발된 당뇨쥐 췌장과 신장조직의 항산화 효소와 형태학적 변화에 미치는 영향감창주, 김성수, 김영표, 김종오, 신말순, 윤진환, 이희혁, 정경훈, 정일규한국운동생리학회 · 운동과학2003 · 제12권 제3호트레드밀 운동이 당뇨 유발 쥐 심근의 단백질 수송체 및 젖산탈수소 동위효소 발현에 미치는 영향윤진환, 이상학한국운동생리학회 · 운동과학2012 · 제21권 제1호STZ-당뇨 흰쥐에서 규칙적인 Treadmill 운동이 골격근 제4형 당수송체에 미치는 영향김용운, 김종연, 박소영, 배형일, 이석강영남대학교 의과대학 · Journal of Yeungnam Medical Science1998 · 제15권 제2호유산소 운동이 제2형 당뇨 쥐 해마에서 미토콘드리아 칼슘 조절과 세포사멸에 미치는 영향박세환, 윤진환한국운동생리학회 · 운동과학2021 · 제30권 제2호

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