Chlortetracycline Caused Vitellogenin Induction at Male Japanese Medaka (Oryzias latipes)
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Abstract
This investigation was intended to find out the estrogenic effect of chlortetracycline (CTC) on vitellogenin induction in adult male Japanese medaka (Oryzias latipes). Vitellogenin (Vtg) produced in male fish has been used to as one of a biomarker of endocrine disrupters. The positive control was 17b-estradiol (E2) that induced Vtg in male fish. As a result, male and female fish were exposed to 0.1, 1, 10 and 100 ppm of CTC. Western blot results showed approximately 205 kDa, that is similar to myosin at high molecular weight range Sigma maker. Vtg band was showed fainted to 10 and 100ppm for chlortetracycline. Vtg concentration of CTC was qunatified by total protein quantification and ELISA. Exposure of the male fish to CTC of 0.1, 1, 10 and 100 ppm produced Vtg concentrations of 0.24, 0.12, 7.61 and 40.02, respectively, that value was elevated than control male fish (0.14). CTC exerted as a Vtg inducer in male fish from 10 ppm, but it was a reducer in female fish from 0.1ppm level. The results say that vitellogenin induction patterns alter in male medaka treated with CTC, and that CTC may caused endocrine disruption in fish.
참고문헌 (11)
- "Are veterinary medicines causing environmental risks", 37(part A), pp.286~294 (2003)
- "The environmental side effects of medication: How are human and veterinary medicines in soils and water bodies affecting human and environmental health", 5, pp.1110~1116 (2004)
- "Veterinary medicines in the environment", 180, pp.1~91 (2004)
- "Pharmaceuticals and personal care products in the environment: agents of subtle change", 107, pp.907~937 (1999)
- "Fecal residues of veterinary parasiticides: nontarget effects in the pasture environment", 50, pp.153~179 (2005)
- "Endocrine disruption induced by some sulfa drugs and tetracyclines on Oryzias latipes", 32(3), pp.227~234 (2006)
- "Several human pharmaceutical residues in aquatic environment may result in endocrine disruption in Japanese Medaka (Oryzias latipes)", 31(3), pp.227~233 (2005)
- "Development and application of a monoclonal antibody-based sandwich ELISA for quantification of Japanese medaka (Oryzias latipes) vitellogenin", 132, pp.161~169 (2002)
- "Color atlas of pharmacology", pp.266~283 (2000), New York
- "Human pharmaceuticals, assessing the impacts on aquatic ecosystems", pp.1~46 (2005), SETAC Press. Pensacola
- "Short-term effects of endocrine-disrupting chemicals on the expression of estrogen-responsive genes in male medaka (Oryzias latipes)", 72, pp.239~249 (2005)