Prevalence and Identification of Cryptosporidium spp. from Swine Slurry
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Cryptosporidium spp. were detected in 17 of 135 swine lagoon samples from five farms by 18S ribosomal DNA locus and PCR. Seventeen positive samples identified were included two distinctive genotypes C. suis and Cryptosporidium sp. based on a small-subunit rRNA gene-based PCR-restriction fragment length polymorphism analysis. Cryptosporidium spp. were detected out of farrowing, farrowing and nursery (mix), and finishing. Prevalence rate was 12.6% with infection rates between 3.7 and 18.5%. We concluded that Cryptosporidium oocysts can persist in treated lagoon and potentially contaminate surface water through improper discharge. This study was undertaken for the evaluation of the infection status of the genotypes of Cryptosporidium spp. in swine lagoon.
Abstract
Cryptosporidium spp. were detected in 17 of 135 swine lagoon samples from five farms by 18S ribosomal DNA locus and PCR. Seventeen positive samples identified were included two distinctive genotypes C. suis and Cryptosporidium sp. based on a small-subunit rRNA gene-based PCR-restriction fragment length polymorphism analysis. Cryptosporidium spp. were detected out of farrowing, farrowing and nursery (mix), and finishing. Prevalence rate was 12.6% with infection rates between 3.7 and 18.5%. We concluded that Cryptosporidium oocysts can persist in treated lagoon and potentially contaminate surface water through improper discharge. This study was undertaken for the evaluation of the infection status of the genotypes of Cryptosporidium spp. in swine lagoon.
참고문헌 (22)
- Tyzzer, E, "A sporozoon found in the peptic glands of the common mouse", Proceedings of the Society for Experimental Biology Medicine, 통권 : 5, pp.12~13 (1907)
- Xiao, L., "Cryptosporidium taxonomy: recent advances and implications for public health", Clinical Microbi- ology Reviews, 17(1), pp.72~97 (2004)
- Kennedy, G. A, "Cryptosporidiosis in three pigs", Journal of the American Veterinary Medical Association, 170, pp.348~350 (1977)
- Izumiyama, S., "Prevalence of C. parvum infections in weaned piglets and fattening porkers in Kanagawa Prefecture", Japan, Revista da Sociedade Brasileira de Medicina Tropical, 54, pp.23~26 (2001)
- Hamnes, I. S., "Occurrence of Cryptosporidium and Giardia in suckling piglets in Norway", Veterinary Parasitology, 141, pp.30~41 (2006)
- Langkjaer, R. B., "Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark", Parasitology, 134, pp.339~350 (2007)
- Ayeni, A. O, "The occurrence and effect of Cryptosporidium species on livestock in Ile-Ife, Nigeria", Tropical Veterinarian, 3, pp.96~100 (1985)
- Enemark, H. L, "Pathogenicity of Cryptosporidium parvum - evalution of an animal infection model", Veterinary Parasitology, 113, pp.35~57 (2003)
- Ryan, U. M., "Identification of a novel Cryptosporidium genotype in pigs", Applied and Environmental Microbiology, 69, pp.3970~3974 (2003)
- Plutzer, J, "Genotype and subtype analyses of Cryptosporidium isolates from cattle in Hungary", Veterinary Parasitology, 146, pp.357~362 (2007)
- Johnson, D. W, "Develpoment of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples", Applied and Environmental Microbiology, 61, pp.3849~3855 (1995)
- 정경석, "국내 임상분리주 Streptococcus pneumoniae의 혈청형에 따른 유전적 상관성", 한국환경보건학회지, 30(1), pp.15~21 (2004), 한국환경보건학회
- Rucker, N. J., "Tracking host sources of Cryptosporidium spp. in raw water for improved health risk assessment", Applied and Environmental Microbiology, 72, pp.3945~3957 (2007)
- Xiao, L., "Prevalence and identity of Cryptosporidium spp. in pig slurry", Applied and Environmental Microbiology, 72, pp.4461~4463 (2006)
- Straw, B. E., "Diseases of swine, 9th edition" (2006), Blackwell publishing
- Upton, S. J., "The species of Cryptosporidium (Apicomplexa : Cryptosporididae) infecting mammals", The Journal of Parasitology, 71, pp.625~629 (1985)
- Morgan, U. M., "Molecular and biological characterization of Cryptosporidium in pigs", Australian Veterinary Journal, 77, pp.44~47 (1999)
- Xiao, L, "Prevalence of Cryptosporidium and Giardia infections on two Ohio pig farms with different management systems", Veterinary Parasitology, 52, pp.331~336 (1994)
- Sanford, S. E., "Enteric cryptosporidial infection in pigs, 184 cases (1981-1985)", Journal of the American Veterinary Medical Association, 190, pp.695~698 (1987)
- Maddox-Hyttel, R. B, "Cryptosporidium and Giardia in different age groups of Danish cattle and pigs-occurrence and management associated risk factors", Veterinary Parasitology, 141, pp.48~59 (2006)
- Olsen, M. E, "Giardia and Cryptosporidium in Canadian farm animals", Veterinary Parasitology, 68, pp.75~381 (1997)
- Fayer, R, "Zoonotic protozoa : from land to sea", Trends in Parasitology, 20, pp.531~536 (2004)